Dual Role of Baimao-Longdan-Congrong-Fang in Inhibiting Staphylococcus aureus Virulence Factors and Regulating TNF-α/TNFR1/NF-κB/MMP9 Axis

Phytomedicine, Год журнала: 2025, Номер 139, С. 156477 - 156477

Опубликована: Фев. 7, 2025

Язык: Английский

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Innovative Application of Medicinal Insects: Employing UHPLC-MS, Bioinformatics, In Silico Studies and In Vitro Experiments to Elucidate the Multi-Target Hemostatic Mechanism of Glenea cantor (Coleoptera: Cerambycidae) Charcoal-Based Medicine

Pharmaceuticals, Год журнала: 2025, Номер 18(4), С. 479 - 479

Опубликована: Март 27, 2025

Background: Longhorn beetles, a widely recognized group of Chinese traditional medicinal insects, are characterized by their notable hemostatic properties. However, the comprehensive understanding potential has been hindered limitations current research methodologies. Methods: This study focuses on species Glenea cantor (Fabricius), which can produce several generations per year, and introduces novel method using microwave carbonization techniques. By employing an in vitro coagulation test, UHPLC-MS, network pharmacology, molecular docking, dynamics simulation, efficacy mechanism action charcoal medicine (GC-CM) were thoroughly studied. Results: In tests showed that GC-CM significantly reduced activated partial thromboplastin time (APTT) prothrombin (PT), indicating its ability to enhance cascade preliminarily confirming (p < 0.01 vs. blank control group). The analysis revealed comprises 453 components, including 137 bioactive components with high human utilization. After predictions via databases such as SwissTargetPrediction deduplication, 215 targets linked specificity identified. These regulate signaling pathways platelet activation, complement cascades, cGMP-PKG. Molecular docking demonstrated strong affinities between key SRC PIK3R1 compounds 2′,6′-dihydroxy 4′-methoxydihydrochalcone, 1-monolinoleoyl-rac-glycerol (binding energy −5 kcal/mol). simulations show good binding capacity core Conclusions: aim this was elucidate material basis GC-CM, offering model for exploring other insect-based resources.

Язык: Английский

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Advances in omics technologies for traditional Chinese medicine in the prevention and treatment of metabolic bone diseases

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Апрель 11, 2025

Metabolic bone disease (MBD), as one of the most severe metabolic disorders, remains a focal point and challenge in medical research. Numerous studies have demonstrated efficacy Traditional Chinese Medicine (TCM) preventing treating MBD. However, inherent complexity TCM metabolites poses significant limitations elucidating their mechanisms action. The advancement omics technologies, including metabolomics, proteomics, transcriptomics, has greatly facilitated research on These approaches enable identification potential biomarkers exploration pathways underlying interventions for Evidence indicates that monomers, single botanical drugs, herbal formulations are effective, safe, well-tolerated MBD prevention treatment. This review summarizes recent applications key findings metabolomics studying It highlights role technologies uncovering relevant under treatment, providing valuable insights clinical references TCM-based strategies managing

Язык: Английский

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Therapeutic potential of Sweroside in postmenopausal osteoporosis: Inhibition of osteoclast differentiation and promotion of osteoclast apoptosis via NF-κB and MAPK pathways

International Immunopharmacology, Год журнала: 2025, Номер 155, С. 114630 - 114630

Опубликована: Апрель 14, 2025

Язык: Английский

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Irisin mitigates osteoporotic-associated bone loss and gut dysbiosis in ovariectomized mice by modulating microbiota, metabolites, and intestinal barrier integrity

BMC Musculoskeletal Disorders, Год журнала: 2025, Номер 26(1)

Опубликована: Апрель 16, 2025

Язык: Английский

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