Mechanism of salidroside promoting testosterone secretion induced by H2O2 in TM3 Leydig cells based on metabolomics and network pharmacology
Frontiers in Chemistry,
Год журнала:
2025,
Номер
13
Опубликована: Фев. 27, 2025
Oxidative
stress-induced
damage
is
a
significant
contributor
to
the
impairment
of
Leydig
cells
in
testes,
potentially
diminishing
secretion
testosterone
and
other
androgens,
thereby
resulting
deficiency.
Salidroside,
principal
bioactive
constituent
derived
from
Rhodiola,
exhibits
potent
antioxidant
properties.
This
study
aims
investigate
underlying
mechanisms
by
which
salidroside
enhances
secretion.
The
investigated
oxidative
TM3
induced
H2O2
demonstrated
that
significantly
decreased
levels
ROS
MDA,
while
increasing
testosterone,
SOD,
GSH.
These
changes
effectively
ameliorated
stress,
mitigated
damage,
protected
cells,
enhanced
Additionally,
UPLC-QE-Orbitrap-MS
was
employed
analyze
metabolomics
identifying
28
distinct
metabolites
associated
metabolic
pathways.
Key
pathways
identified
include
Arginine
biosynthesis,
Alanine,
aspartate
glutamate
metabolism,
Citrate
cycle
(TCA
cycle),
Phenylalanine
Pyruvate
metabolism.
Utilizing
network
pharmacology,
core
targets
enhancing
were
further
investigated,
revealing
involvement
AMACR,
CYP3A4,
ECHS1,
HSD17B10,
MPO,
TYR.
discovery
confirmed
dry-wet
analysis.
To
sum
up,
can
reduce
level
stress
promote
through
multiple
targets.
In
word,
may
provide
new
strategy
for
preventing
treating
Язык: Английский
Advances in omics technologies for traditional Chinese medicine in the prevention and treatment of metabolic bone diseases
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 11, 2025
Metabolic
bone
disease
(MBD),
as
one
of
the
most
severe
metabolic
disorders,
remains
a
focal
point
and
challenge
in
medical
research.
Numerous
studies
have
demonstrated
efficacy
Traditional
Chinese
Medicine
(TCM)
preventing
treating
MBD.
However,
inherent
complexity
TCM
metabolites
poses
significant
limitations
elucidating
their
mechanisms
action.
The
advancement
omics
technologies,
including
metabolomics,
proteomics,
transcriptomics,
has
greatly
facilitated
research
on
These
approaches
enable
identification
potential
biomarkers
exploration
pathways
underlying
interventions
for
Evidence
indicates
that
monomers,
single
botanical
drugs,
herbal
formulations
are
effective,
safe,
well-tolerated
MBD
prevention
treatment.
This
review
summarizes
recent
applications
key
findings
metabolomics
studying
It
highlights
role
technologies
uncovering
relevant
under
treatment,
providing
valuable
insights
clinical
references
TCM-based
strategies
managing
Язык: Английский
Update on the correlation between mitochondrial function and osteonecrosis of the femoral head osteocytes
Redox Report,
Год журнала:
2025,
Номер
30(1)
Опубликована: Апрель 18, 2025
Mitochondrial
health
is
maintained
in
a
steady
state
through
mitochondrial
dynamics
and
autophagy
processes.
Recent
studies
have
identified
healthy
mitochondria
as
crucial
regulators
of
cellular
function
survival.
This
process
involves
adenosine
triphosphate
(ATP)
synthesis
by
oxidative
phosphorylation
(OXPHOS),
regulation
calcium
metabolism
inflammatory
responses,
intracellular
stress
management.
In
the
skeletal
system,
they
participate
behaviors
responses
osteoblasts,
osteoclasts,
chondrocytes,
osteocytes
to
external
stimuli.
Indeed,
damage
or
dysfunction
occurs
development
few
bone
diseases.
For
example,
may
lead
an
imbalance
osteoblasts
resulting
osteoporosis,
osteomalacia,
poor
production,
chondrocyte
death
infiltration
osteoarthritis
are
main
causes
cartilage
degeneration
due
damage.
However,
opposite
exists
for
osteosarcoma,
where
overactive
able
accelerate
proliferation
migration
osteosarcoma
cells,
which
major
disease
feature.
Bone
dynamic
organ
play
fundamental
role
all
regions
tissue
involved
regulating
integrity.
review
examines
impact
physiological
on
osteocyte
summarizes
microscopic
molecular
mechanisms
underlying
its
effects.
It
highlights
that
targeted
therapies
focusing
be
beneficial
survival,
providing
new
insight
diagnosis,
prevention,
treatment
diseases
associated
with
death.
Язык: Английский
Revealing the Landscape Crosstalk Between Reproductive System and Organs Aging
The FASEB Journal,
Год журнала:
2025,
Номер
39(8)
Опубликована: Апрель 27, 2025
ABSTRACT
The
reproductive
system
is
a
vital
component
of
the
human
body.
In
modern
society,
due
to
various
socio‐economic
reasons,
an
increasing
number
couples
are
choosing
postpone
childbearing.
Research
into
impact
aging
on
becoming
increasingly
important.
As
people
age,
there
decline
in
across
levels,
from
testes
males
spermatogonia
cells,
and
ovaries
females
oocytes.
not
only
affects
itself
but
also
has
implications
for
other
organs
systems
Conversely,
can
damage
system.
This
review
organizes
changes
that
occur
within
as
result
focuses
interactions
between
systems.
Additionally,
this
summarizes
current
therapies
aimed
at
delaying
aging,
which
may
provide
insights
future
interventions
targeting
Язык: Английский