Accurate Quantification of Metals in Individual Synechocystis sp. PCC 6803 Cells by Single-Cell ICP-MS: Dual-Calibration and Sample Stabilization Strategies DOI
Junhui Zhang, Lihong Liu, Qinfei Zhou

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 13, 2025

Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) is an emerging technique to investigate metal heterogeneity in individual cells. However, due the absence of consistent calibration and suitable stabilization strategy for cells, accurate quantification cellular content metals remains a challenge. Herein, method heterogeneous distribution among microalgae cells was developed based on SC-ICP-MS using dual-calibration strategies robust pretreatment methods. Gold nanoparticles (AuNPs) were used as measuring contents single but it would lead 13.6-63.1% underestimation cell numbers inaccurate detection cells' transport efficiency. To avoid this inaccuracy, we proposed additional measure efficiency endogenous Mg, enabling more assessment heterogeneity. Then, effective optimized through fixation with glutaraldehyde 1 h maintain stability obtain results, satisfactory recoveries number (98.4%) Mg (91.7%), even after long-time storage. After optimization, showed high sensitivity repeatability both (Mg, Hg, Cd, Co) number, limits (LODs) be 0.14-0.53 fg/cell 5.5 × 103 cells/mL, respectively. Finally, successfully detecting various their Synechocystis sp. PCC 6803 provided tool investigating uptake microalgae.

Язык: Английский

Lead biosorption and chemical composition of extracellular polymeric substances isolated from mixotrophic microalgal cultures DOI Creative Commons
Wioleta Ciempiel, Magdalena Czemierska, Dariusz Wiącek

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 17, 2025

Abstract Extracellular polymers (EPS) produced by microalgae are considered an important factor in the process of biosorption environmental contaminants. The study investigated impact mixotrophic cultivation unicellular algae Chlorella vulgaris , Parachlorella kessleri and Vischeria magna on specific productivity yield total soluble EPS as well biochemical composition sorption properties extracellular order to explore their potential be used for biosorption. results showed that conditions enhanced contributed changes monomer EPS. Higher levels sugars, reducing protein, phenolic compounds reduced content uronic acids were observed isolated conditions. Rhamnose, xylose, mannose, glucose, galactose detected samples. FTIR ICP-OES applied characterise structure role Pb(II) removal. carboxyl groups hydroxyl played process. from C. cultures highest removal capacity.

Язык: Английский

Процитировано

1
Research hotspots and application of algal extracellular polymeric substances (EPS) in wastewater treatment and resources recovery DOI
Jiawei Xiang, Liangang Hou,

Zhengwei Pan

и другие.

Desalination, Год журнала: 2024, Номер unknown, С. 118510 - 118510

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

5
Identification of Camellia Oil Adulteration With Excitation-Emission Matrix Fluorescence Spectra and Deep Learning DOI
Chaojie Wei, Zhao‐Jun Wei,

Yanna Jiao

и другие.

Journal of Fluorescence, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Язык: Английский

Процитировано

0
Adaptation responses of Dunaliella sp. and its natural symbiotic bacteria to sulfamethoxazole DOI Creative Commons
Xiao Tan, Gang Pan, Wenxue Che

и другие.

Environmental Technology & Innovation, Год журнала: 2025, Номер unknown, С. 104171 - 104171

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
The combined effect of phosphorus forms and zinc on arsenic toxicity and bioaccumulation in Phaeodactylum tricornutum DOI
Jian‐Di Li, Liping Jia, Yongqiang Tian

и другие.

Algal Research, Год журнала: 2025, Номер unknown, С. 104097 - 104097

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Accurate Quantification of Metals in Individual Synechocystis sp. PCC 6803 Cells by Single-Cell ICP-MS: Dual-Calibration and Sample Stabilization Strategies DOI
Junhui Zhang, Lihong Liu, Qinfei Zhou

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 13, 2025

Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) is an emerging technique to investigate metal heterogeneity in individual cells. However, due the absence of consistent calibration and suitable stabilization strategy for cells, accurate quantification cellular content metals remains a challenge. Herein, method heterogeneous distribution among microalgae cells was developed based on SC-ICP-MS using dual-calibration strategies robust pretreatment methods. Gold nanoparticles (AuNPs) were used as measuring contents single but it would lead 13.6-63.1% underestimation cell numbers inaccurate detection cells' transport efficiency. To avoid this inaccuracy, we proposed additional measure efficiency endogenous Mg, enabling more assessment heterogeneity. Then, effective optimized through fixation with glutaraldehyde 1 h maintain stability obtain results, satisfactory recoveries number (98.4%) Mg (91.7%), even after long-time storage. After optimization, showed high sensitivity repeatability both (Mg, Hg, Cd, Co) number, limits (LODs) be 0.14-0.53 fg/cell 5.5 × 103 cells/mL, respectively. Finally, successfully detecting various their Synechocystis sp. PCC 6803 provided tool investigating uptake microalgae.

Язык: Английский

Процитировано

0