El
objetivo
fue
evaluar
el
efecto
crioprotector
del
tipo
de
azúcar
en
diluyente
sobre
la
criopreservación
semen
cerdo
pelón
mexicano.
Se
obtuvieron
30
eyaculados
cinco
verracos
sexualmente
maduros,
los
cuales
fueron
mezclados,
diluidos
y
congelados
cuatro
diferentes
crioprotectores:
glucosa
(T1),
lactosa
(T2),
fructosa
(T3)
trehalosa
(T4).
La
descongelación
se
realizó
a
37
°C
evaluaron
parámetros
motilidad
(sistema
CASA),
viabilidad
espermática
(IP),
actividad
mitocondrial
(JC-1),
integridad
acrosoma
(FITC-PSA)
membrana
plasmática
cola
(Host).
Motilidad
total
(MT)
velocidad
trayectoria
media
(VAP)
superiores
(P
<
0.02)
T1
T2,
mientras
que
curvilínea
(VCL)
mayor
0.05)
T1.
Las
otras
variables
asociadas
similares
(P>0.05)
al
igual
cola.
0.005)
T2
T4.
(P<0.001)
En
conclusión,
con
es
más
adecuado
para
http://dx.doi.org/10.21929/abavet2023.23
e2022-39
https://www.youtube.com/watch?v=n-SwMBul4Hg
Frontiers in Veterinary Science,
Год журнала:
2025,
Номер
11
Опубликована: Янв. 6, 2025
The
motility
of
sperm
decreases
following
cryopreservation,
which
is
closely
associated
with
mitochondrial
function.
However,
the
alterations
in
metabolism
after
freezing
goats
remain
unclear.
This
experiment
aimed
to
investigate
impact
ultra-low
temperature
on
goat
sperm's
energy
and
its
potential
correlation
motility.
results
revealed
that
exhibited
vacuolization,
reduced
matrix
density,
significantly
decreased
levels
high-membrane
mitochondria
adenosine
triphosphate
content,
accompanied
by
a
substantial
increase
reactive
oxygen
species
levels,
ultimately
leading
significant
decline
viability.
Further
investigations
unveiled
energy-related
differential
metabolites
(capric
acid,
creatine,
D-glucosamine-6-phosphate)
antioxidant
effects
(saikosaponin
A,
probucol,
cholesterol
sulfate)
were
downregulated.
In
addition,
activity
key
rate-limiting
enzymes
involved
very
long-chain
fatty
acid
biosynthesis
β-oxidation-specifically
acetyl-CoA
carboxylase,
synthase,
carnitine
palmitoyltransferase
I
related
capric
metabolism-was
considerably
reduced.
Furthermore,
supplementation
metabolite
(500
μM)
enhanced
frozen-thawed
sperm.
These
findings
indicated
ultrastructure
damaged
becomes
abnormal
potentially
affecting
addition
different
such
as
extender
can
alleviate
decrease
induced
cryopreservation.
Indian Journal of Animal Research,
Год журнала:
2025,
Номер
Of
Опубликована: Янв. 13, 2025
Apoptosis
is
an
integral
part
of
sperm
development,
quality
control
and
differentiation.
Caspases
are
proteolytic
enzymes
that
play
a
key
role
in
apoptosis.
The
aim
this
review
article
to
analyze
the
caspases
3,
8
9
male
infertility
cryopreservation.
also
discusses
effect
cryopreservation
on
activation
sperm,
leading
apoptosis
decreased
quality.
Finally,
explores
use
caspase
inhibitors
as
strategy
improve
protocols
preserve
Caspase
3
dominant
effector
caspase,
while
involved
extrinsic
intrinsic
apoptotic
pathways,
respectively.
Increased
activity
these
has
been
associated
with
various
issues,
such
teratozoospermia,
asthenozoospermia,
oligozoospermia
reduced
cryostability.
emphasizes
importance
understanding
their
potential
diagnostic
markers
therapeutic
targets
for
Foods,
Год журнала:
2025,
Номер
14(9), С. 1507 - 1507
Опубликована: Апрель 25, 2025
Eggs,
recognized
as
a
"complete
nutritional
food",
constitute
crucial
source
of
high-quality
protein
and
maintain
an
essential
position
in
China's
animal
supply
system.
However,
extended
storage
periods
induce
biochemical
degradation,
including
denaturation,
air
cell
expansion,
microbial
growth,
substantially
affecting
both
food
safety
value.
As
consumer
demand
for
quality
assurance
increases,
research
egg
freshness
evaluation
has
made
substantial
progress.
While
existing
studies
have
focused
on
isolated
detection
methods
freshness,
there
remains
critical
gap
systematically
integrating
multidisciplinary
approaches
evaluating
their
synergistic
potential
comprehensive
assessment.
This
review
categorizes
conventional
emerging
methodologies,
sensory
assessment,
physical
characterization,
chemical
analysis,
intelligent
technologies.
The
paper
presents
analysis
current
developments
while
offering
perspectives
practical
applications
future
directions
systems.
Biopreservation and Biobanking,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 26, 2024
Introduction:
Sperm
cryopreservation
is
a
useful
storage
technique
in
artificial
insemination.
Nanoparticles
and
nanovesicles
such
as
exosomes
are
widely
used
sperm
procedures
to
alleviate
cold-induced
injury
inflicted
during
freezing.
Journal of the Indonesian Tropical Animal Agriculture,
Год журнала:
2024,
Номер
49(2), С. 108 - 116
Опубликована: Март 3, 2024
Preservation
of
the
livestock
genetic
makeup
could
be
performed
by
application
in
vitro
fertilization
(IVF)
using
spermatozoa
collected
from
cauda
epididymis.
Therefore,
this
study
as
sessed
development
goat
embryos
following
IVF
epididy
mis
compared
to
those
kept
frozen.
Oocytes
were
cultured
a
maturation
medium
for
24
hours
at
temperature
38.5°C,
5%
CO2,
and
95%
humidity.
Following
maturation,
oocytes
(n
=
370)
fertilized
fresh
epididymal
sperm
(G1)
G2
frozen-thawed
sperm,
Fert-TALP
was
used
medium.
In
addition,
developed
GT-L
The
rate,
percentage
morula,
blastocytes
(p<0.01)
significantly
higher
inseminated
with
than
frozen
sperm.
conclusion,
embryo
production
may
successfully
IVF.
Reproduction in Domestic Animals,
Год журнала:
2024,
Номер
59(S3)
Опубликована: Окт. 1, 2024
Abstract
A
protocol
for
conventional
in
vitro
fertilization
(IVF)
horses
using
fresh
semen
has
been
described,
a
prolonged
incubation
FERT‐TALP
medium
(22
h)
at
38.2°C
the
presence
of
penicillamine,
hypotaurine
and
epinephrine
(PHE).
Our
work
aimed
to
develop
that
maintains
quality
parameters
frozen–thawed
equine
spermatozoa
incubated
22
h
PHE
different
media
(FERT‐TALP
INRA96)
temperatures
(30
38.2°C).
Twelve
frozen
ejaculates
from
four
stallions
were
thawed
then
either
or
INRA96
with
30
h.
Following
incubation,
total
motility
(TM),
progressive
(PM),
viability
acrosome
integrity
evaluated.
The
results
showed
TM
was
significantly
higher
(
p
<
.001)
30°C
both
media,
while
PM
compared
38°C
.05).
Moreover,
exhibited
sperm
other
experimental
groups.
These
preliminary
suggest
incubating
successfully
motility,
spermatozoa,
indicating
its
potential
use
IVF.
