Immunoglobulin Y-Based Lateral Flow Immunoassay Strip Test for Detecting Ciprofloxacin Antibiotic in Raw Pork Samples DOI Creative Commons
Sumed Yadoung,

Huan-Yuan Xu,

Sirikwan Dokuta

и другие.

Foods, Год журнала: 2025, Номер 14(5), С. 818 - 818

Опубликована: Фев. 27, 2025

Ciprofloxacin is metabolized from enrofloxacin for use in poultry to manage respiratory and gastrointestinal diseases, raising concerns due its widespread tissue distribution prolonged systemic persistence. This lateral flow immunoassay was designed detect ciprofloxacin using an alternative IgY antibody binded with gold nanoparticles residue raw pork meat samples. The developed strip test achieved adequate sensitivity specificity under the optimized conditions pH, which 7.8, 20% of MeOH 0.01 M phosphate buffer containing 1% Tween-20 used composition. An concentration 1.25 µg/mL bind as a probe detection. line (coating antigen) control (anti-IgY secondary antibody) 0.5 mg/mL 0.2 mg/mL, respectively. efficiency showed 50% inhibitory (IC50) at 7.36 µg/mL, limit detection µg/mL. proposed strategy exhibited potential monitoring

Язык: Английский

Immunoglobulin Y-Based Lateral Flow Immunoassay Strip Test for Detecting Ciprofloxacin Antibiotic in Raw Pork Samples DOI Creative Commons
Sumed Yadoung,

Huan-Yuan Xu,

Sirikwan Dokuta

и другие.

Foods, Год журнала: 2025, Номер 14(5), С. 818 - 818

Опубликована: Фев. 27, 2025

Ciprofloxacin is metabolized from enrofloxacin for use in poultry to manage respiratory and gastrointestinal diseases, raising concerns due its widespread tissue distribution prolonged systemic persistence. This lateral flow immunoassay was designed detect ciprofloxacin using an alternative IgY antibody binded with gold nanoparticles residue raw pork meat samples. The developed strip test achieved adequate sensitivity specificity under the optimized conditions pH, which 7.8, 20% of MeOH 0.01 M phosphate buffer containing 1% Tween-20 used composition. An concentration 1.25 µg/mL bind as a probe detection. line (coating antigen) control (anti-IgY secondary antibody) 0.5 mg/mL 0.2 mg/mL, respectively. efficiency showed 50% inhibitory (IC50) at 7.36 µg/mL, limit detection µg/mL. proposed strategy exhibited potential monitoring

Язык: Английский

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