The flavonoid quercetin decreases ACE2 and TMPRSS2 expression but not SARS‐CoV‐2 infection in cultured human lung cells DOI Creative Commons
Michael J. Houghton, Églantine Balland, Matthew J. Gartner

и другие.

BioFactors, Год журнала: 2024, Номер 50(6), С. 1268 - 1286

Опубликована: Июнь 17, 2024

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) binds to angiotensin-converting enzyme (ACE2) on host cells, via its spike protein, and transmembrane protease, serine (TMPRSS2) cleaves the spike-ACE2 complex facilitate virus entry. As rate-limiting steps for entry, modulation of ACE2 and/or TMPRSS2 may decrease SARS-CoV-2 infectivity COVID-19 severity. In silico modeling suggested natural bioactive flavonoid quercetin can bind a recent randomized clinical trial demonstrated that oral supplementation with increased recovery. A range cultured human cells were assessed co-expression TMPRSS2. Immortalized Calu-3 lung matured at an air-liquid interface (Calu-3-ALIs), established as most appropriate. Primary bronchial epithelial (PBECs) obtained from healthy adult males (N = 6) under submerged conditions corroborate outcomes. Upon maturation or reaching 80% confluence, respectively, Calu-3-ALIs PBECs treated quercetin, mRNA protein expression by droplet digital PCR ELISA, respectively. infectivity, effects pre- co-treatment was median tissue culture infectious dose assay. Quercetin dose-dependently decreased in both after 4 h, while remained suppressed response prolonged treatment lower doses (twice daily 3 days). also acutely ADAM17 mRNA, but not ACE, Calu-3-ALIs, this warrants further investigation. PBECs, successfully infected SARS-CoV-2; however, had no antiviral effect, neither directly nor indirectly through downregulation reaffirmed be optimal cell model research into regulation TMPRSS2, without need prior genetic modification, will prove valuable future disease work. However, our data demonstrate significant promising prophylactic candidate translate infection prevention.

Язык: Английский

A Systematic Review: Quercetin—Secondary Metabolite of the Flavonol Class, with Multiple Health Benefits and Low Bioavailability DOI Open Access
Olimpia Daniela Frenț,

Liana Ștefan,

Claudia Mona Morgovan

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12091 - 12091

Опубликована: Ноя. 11, 2024

The main goal of this systematic review on the flavonol class secondary metabolite quercetin is to evaluate and summarize existing research quercetin's potential health benefits, therapeutic properties, effectiveness in disease prevention treatment. In addition evaluating for drug development with fewer side effects lower toxicity, type attempts collect scientific evidence addressing roles as an antioxidant, anti-inflammatory, antibacterial, anticancer agent. first part, we analyze various flavonoid compounds, focusing their chemical structure, classification, natural sources. We highlight most recent biological activities reported literature. Among these pay special attention quercetin, detailing its physicochemical process biosynthesis plants. also present sources emphasize such antioxidant anti-inflammatory effects. Additionally, discuss methods enhance bioavailability, analyzing latest effective delivery systems based quercetin.

Язык: Английский

Процитировано

7

In-silico exploring pathway and mechanism-based therapeutics for allergic rhinitis: Network pharmacology, molecular docking, ADMET, quantum chemistry and machine learning based QSAR approaches DOI
K.M. Tanjida Islam, Shahin Mahmud

Computers in Biology and Medicine, Год журнала: 2025, Номер 187, С. 109754 - 109754

Опубликована: Фев. 4, 2025

Язык: Английский

Процитировано

0

Identification and Inhibition Mechanism of Novel Collagen-Derived Hyaluronidase Inhibitory Peptides DOI
Wenjun Xue,

Xiaoyu Kuang,

Xiangyu Meng

и другие.

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106263 - 106263

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Micro- and nano-bubble mediated ultrasound-assisted extraction of quercetin from red onion peels using glycerol–water solvent DOI
E Yang, In Sook Lee, Hyo Sung Jung

и другие.

Journal of Molecular Liquids, Год журнала: 2025, Номер unknown, С. 127492 - 127492

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

The flavonoid quercetin decreases ACE2 and TMPRSS2 expression but not SARS‐CoV‐2 infection in cultured human lung cells DOI Creative Commons
Michael J. Houghton, Églantine Balland, Matthew J. Gartner

и другие.

BioFactors, Год журнала: 2024, Номер 50(6), С. 1268 - 1286

Опубликована: Июнь 17, 2024

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) binds to angiotensin-converting enzyme (ACE2) on host cells, via its spike protein, and transmembrane protease, serine (TMPRSS2) cleaves the spike-ACE2 complex facilitate virus entry. As rate-limiting steps for entry, modulation of ACE2 and/or TMPRSS2 may decrease SARS-CoV-2 infectivity COVID-19 severity. In silico modeling suggested natural bioactive flavonoid quercetin can bind a recent randomized clinical trial demonstrated that oral supplementation with increased recovery. A range cultured human cells were assessed co-expression TMPRSS2. Immortalized Calu-3 lung matured at an air-liquid interface (Calu-3-ALIs), established as most appropriate. Primary bronchial epithelial (PBECs) obtained from healthy adult males (N = 6) under submerged conditions corroborate outcomes. Upon maturation or reaching 80% confluence, respectively, Calu-3-ALIs PBECs treated quercetin, mRNA protein expression by droplet digital PCR ELISA, respectively. infectivity, effects pre- co-treatment was median tissue culture infectious dose assay. Quercetin dose-dependently decreased in both after 4 h, while remained suppressed response prolonged treatment lower doses (twice daily 3 days). also acutely ADAM17 mRNA, but not ACE, Calu-3-ALIs, this warrants further investigation. PBECs, successfully infected SARS-CoV-2; however, had no antiviral effect, neither directly nor indirectly through downregulation reaffirmed be optimal cell model research into regulation TMPRSS2, without need prior genetic modification, will prove valuable future disease work. However, our data demonstrate significant promising prophylactic candidate translate infection prevention.

Язык: Английский

Процитировано

2