Adoptive transfer of natural killer cells in therapeutic treatment of COVID-19 patients
Egyptian Journal of Medical Human Genetics,
Год журнала:
2025,
Номер
26(1)
Опубликована: Фев. 22, 2025
Abstract
Background
Natural
killer
(NK)
cells
are
crucial
constituents
of
innate
immunity,
playing
a
vital
role
in
the
early
defense
against
viral
infections
and
cancer.
Their
antiviral
capabilities
stem
from
direct
cytotoxic
activity
infected
immunoregulatory
functions
that
modulate
adaptive
immune
responses.
In
context
Coronavirus
disease
(COVID-19),
NK
contribute
to
controlling
replication
limiting
excessive
inflammatory
Aims
This
review
highlights
mechanisms
underlying
cell-mediated
activity,
focusing
on
their
interactions
with
severe
acute
respiratory
syndrome
coronavirus
2
(SARS‑CoV‑2).
We
discuss
potential
sources
for
adoptive
transfer
therapy,
critical
factors
influencing
therapeutic
efficacy,
considerations
clinical
translation
COVID-19
treatment.
Methods
Various
cell
therapy
discussed,
including
peripheral
blood,
cord
genetically
engineered
cells.
Key
efficacy
safety
cell-based
therapies
analyzed,
cytokine
modulation,
subset
selection,
delivery
methods.
Conclusion
Understanding
influence
success
can
inform
design
more
effective
immunotherapies
patients.
Strategies
improving
function,
such
as
genetic
modification
optimizing
protocols,
highlighted
enhance
outcomes.
Graphical
neutralize
SARS-CoV-2
by
releasing
granules,
inhibiting
replication,
modulating
inflammation.
They
originate
engineering.
Therapeutic
strategies
include
transfer,
optimized
delivery.
Inflammation
is
controlled,
reducing
Язык: Английский
A Stable mRNA-Based Novel Multi-Epitope Vaccine Designs Against Infectious Heartland Virus by Integrated Immunoinformatics and Reverse Vaccinology Approaches
Viral Immunology,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 24, 2025
The
Heartland
virus
(HRTV)
is
a
tick-borne
human
pathogenic
phlebovirus
that
primarily
causes
leukopenia
and
thrombocytopenia.
It
transmitted
by
Amblyomma
americanum
type
of
tick,
is,
notable
for
their
aggressive
biting
behavior,
affinity
hosts,
high
prevalence.
Developing
vaccines
or
immunizations
against
HRTV
gaining
importance
as
public-health
preventive
strategy.
current
study
was
planned
to
prioritize
multi-epitope
stable
mRNA
vaccine
model
from
lead
B-cell
T-cell
epitopes
(with
IC50
<
100
nM)
proteome
following
advanced
immunoinformatics
approaches.
Model
constructs
were
designed
linking
the
most
potent,
nonallergenic
along
with
incorporation
ribosomal
protein
adjuvant
immune
response
enhancement.
immunogenic
potential
coding
molecule
examined
via
molecular
docking
toll-like
receptors
followed
normal
mode
analysis
dynamics
simulations-based
energy
minimization,
stability,
flexibility
assessments.
A
robust,
circular
precursor
multi-epitopes
incorporating
Kozak
consensus
sequence,
start
codon,
essential
elements
such
MHC
class
I
trafficking
domain
(MITD),
tPA,
Goblin
5′
3′
Untranslated
Region
(UTRs),
poly
(A)
tail.
This
strategic
amalgamation
ensures
elevated
immunogenicity
predicts
promising
HRTV.
simulation
predicted
capable
elicit
cell-mediated
humoral
responses.
examine
experimentally
its
safety
features.
Язык: Английский