iScience, Год журнала: 2025, Номер 28(3), С. 111977 - 111977
Опубликована: Фев. 8, 2025
In teleost fish, primordial germ cells (PGCs), the precursors of eggs and sperm, develop from that inherit maternal plasm (GP). Although numerous GP component genes have been identified, minimum set essential for germline formation remains unknown. Herein, we provide evidence dnd1 nanos3 synergistically induce PGC-like blastomeres medaka embryos. One-cell stage embryos injected with mRNA (DN-OE) showed developmental arrest before gastrulation upregulation PGC markers. Transplantation experiments revealed most transplanted DN-OE migrated into gonadal ridge host embryos, resulting in production functional sperm. Furthermore, combination genome editing induction techniques successfully generated transgenic knock-in medaka, demonstrating enhanced, precise, stable gene integration. Identifying advances our understanding cell development mechanisms their biotechnological applications.
Язык: Английский