Polydopamine-based nanozyme with dual-recognition strategy-driven fluorescence-colorimetric dual-mode platform for Listeria monocytogenes detection

Journal of Hazardous Materials, Год журнала: 2022, Номер 439, С. 129582 - 129582

Опубликована: Июль 12, 2022

Язык: Английский

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Confined construction of COF@Cu-nanozyme with high activity and stability as laccase biomimetic catalyst for the efficient degradation of phenolic pollutants

Chemical Engineering Journal, Год журнала: 2022, Номер 448, С. 137701 - 137701

Опубликована: Июнь 21, 2022

Язык: Английский

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Amino-Ligand-Coordinated Dicopper Active Sites Enable Catechol Oxidase-Like Activity for Chiral Recognition and Catalysis

Nano Letters, Год журнала: 2023, Номер 23(2), С. 701 - 709

Опубликована: Янв. 4, 2023

Developing highly active and selective advanced nanozymes for enzyme-mimicking catalysis remains a long-standing challenge basic research practical applications. Herein, we grafted chiral histidine- (His-) coordinated copper core onto Zr-based metal–organic framework (MOF) backbones to structurally mirror the bimetal site of natural catechol oxidase. Such biomimetic fabricated process affords MOF-His-Cu with oxidase-like activity, which can catalyze dehydrogenation oxidation o-diphenols then transfer electrons O2 generate H2O2 by cyclic conversion Cu(II) Cu(I). Specifically, elaborate incorporation His arms results in higher catalytic selectivity over substrates than enzyme. Density functional theory calculations reveal that binding energy potential steric effect site-substrate interactions account high stereoselectivity. This work demonstrates efficient processes deepens understanding mechanism nanozymes.

Язык: Английский

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Nanozymes: powerful catalytic materials for environmental pollutant detection and degradation

Environmental Science Nano, Год журнала: 2024, Номер 11(3), С. 766 - 796

Опубликована: Янв. 1, 2024

Here we made a critical review on nanozyme-involved detection and degradation of environmental pollutants, the research progress achieved in last five years was emphatically concluded.

Язык: Английский

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Bioinspired Coassembly of Copper Ions and Nicotinamide Adenine Dinucleotides for Single-Site Nanozyme with Dual Catalytic Functions

Analytical Chemistry, Год журнала: 2023, Номер 95(5), С. 2865 - 2873

Опубликована: Янв. 24, 2023

Nanozymes can imitate the catalytic properties of natural enzymes while overcoming limitations such as high cost, poor robustness, and difficulty in recycling. However, rational design facile preparation nanozymes are still demand. Inspired by chemical structure laccase, we report an amorphous metal-organic coordination nanocomposite named CuNAD, which is composed copper ions nicotinamide adenine dinucleotide (NAD+) via a simple coordinating coassembly process. As single-site nanozyme, CuNAD exhibits excellent robustness under extreme conditions, significantly stronger activity for phenolic compounds, 4.02-fold higher sensitivity epinephrine detection than laccase. Furthermore, breaking through functional constraints also able to activate H2O2 at neutral pH, benefiting one-step chromogenic platform cholesterol. This approach demonstrates potential develop biomimicking may boost more insights into structure-function relationship nanozymes.

Язык: Английский

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Multifunctional Manganese–Nucleotide Laccase-Mimicking Nanozyme for Degradation of Organic Pollutants and Visual Assay of Epinephrine via Smartphone

Analytical Chemistry, Год журнала: 2024, Номер 96(11), С. 4736 - 4744

Опубликована: Март 11, 2024

As a natural green catalyst, laccase has extensive application in the fields of environmental monitoring and pollutant degradation. However, susceptibility to influences poor reusability seriously hinder its application. To address these concerns, for first time, manganese ion replaced copper as active center coordinate with guanosine monophosphate (GMP) synthesizing mimic high catalytic activity. Compared laccase, laccase-like nanozyme (Mn–GMPNS) demonstrated superior thermal stability, acid–base resistance, salt tolerance, reusability, substrate universality. Benefiting from activity Mn–GMPNS, epinephrine, significant neurotransmitter hormone associated numerous diseases, was visually detected within 10 min portable assay by smartphone. More encouragingly, Mn–GMPNS can efficiently degrade dye pollutants, achieving decolorization rate over 70% 30 min. Thus, coordination between nucleotide potential rational design nanozymes activity, low cost, good biocompatibility.

Язык: Английский

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Self-Assembled Integrated Nanozyme Cascade Biosensor with Dual Catalytic Activity for Portable Urease Analysis

Analytical Chemistry, Год журнала: 2024, Номер 96(3), С. 1284 - 1292

Опубликована: Янв. 9, 2024

In this work, a novel nanozyme (Cu@Zr) with all-in-one dual enzyme and fluorescence properties is designed by simple self-assembly. A cascade sensor disodium phenyl phosphate (PPDS) as substrate was first established exploiting the enzymatic activities of phosphatase laccase. Specifically, cleaves P–O bond PPDS to produce colorless phenol, which then oxidized laccase complexed chromogenic agent 4-aminoantipyrine (4-AP) red quinoneimine (QI). Strikingly, NH3 produced urease hydrolysis urea can interact Cu@Zr, accelerating electron transfer rate ultimately leading significantly improved performance reaction. Moreover, at 440 nm Cu@Zr further quenched inner filter effect (IFE) QI. Thus, colorimetric dual-mode strategy for sensitive analysis LODs 3.56 1.83 U/L proposed sensor. Notably, portable swab loaded also prepared in situ detection aid smartphone RGB readout. It provides potentially viable analytical avenue environmental biological analysis.

Язык: Английский

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Biomimetic Electrochemical Sensor Based on Single-Atom Nickel Laccase Nanoenzyme for Quercetin Detection

Analytical Chemistry, Год журнала: 2024, Номер 96(6), С. 2610 - 2619

Опубликована: Фев. 2, 2024

Laccase, a member of the copper oxidase family, has been used as green catalyst in environmental and biochemical industries. However, laccase nanoenzymes are limited to materials with active site, noncopper have scarcely reported. In this study, inspired by multiple sites natural redox Cu2+/Cu+ electron transfer pathway, novel nitrogen/nickel single-atom nanoenzyme (N/Ni SAE) high laccase-like activity was prepared inducing Ni dopamine precipitation through controllable water/ethanol interface reaction. Compared that laccase, simulated N/Ni SAE exhibited excellent stability reusability. The higher efficiency toward degradation 2,4-dichlorophenol, hydroquinone, bisphenol A, p-aminobenzene. addition, sensitive electrochemical biosensor constructed leveraging SAE; sensor offered unique advantages terms catalytic activity, selectivity, stability, repeatability. Its detection ranges for quercetin were 0.01–0.1 1.0–100 μM, limit 3.4 nM. It also successfully quantitative fruit juices. Therefore, biomimetic study promote development new strategy various bioactive molecules show great potential practical applications.

Язык: Английский

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MnO2 nanosheets based catechol oxidase mimics for robust electrochemical sensor: Synthesis, mechanism and its application for ultrasensitive and selective detection of dopamine

Chemical Engineering Journal, Год журнала: 2024, Номер 493, С. 152656 - 152656

Опубликована: Май 28, 2024

Язык: Английский

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Porous, Oxygen Vacancy Enhanced CeO_2–x Microspheres with Efficient Enzyme-Mimetic and Photothermal Properties

ACS Sustainable Chemistry & Engineering, Год журнала: 2022, Номер 10(29), С. 9492 - 9505

Опубликована: Июнь 15, 2022

Porous, defective, gray cerium oxide (g-CeO2–x) microspheres 4.8 μm in size were synthesized as a multifunctional nanozyme with catalase-, peroxidase-, and oxidase-like activities by the reduction of monodisperse-porous (CeO2) microspheres. Higher Ce(III) atomic fraction, more oxygen vacancy, lower content on surface g-CeO2–x shown Raman X-ray photoelectron spectroscopy. Band gap energies plain CeO2 determined 3.0 2.4 eV, respectively. Reactive species (ROS) related to enzyme-mimetic activity singlet (1O2•) superoxide anion (•O2–) ESR Michaelis–Menten plots sketched for provided superior maximum substrate consumption rates Oxidase- peroxidase-like used developing colorimetric fluorometric protocols detection nitrite common pollutant, also exhibited photothermal response explained enhanced light adsorption originated from vacancies. A temperature elevation up 19 °C was obtained under near infrared laser irradiation at 808 nm. Photothermal accompanying makes porous promising synergistic therapy agent capable overcoming hypoxia generating additional ROS tumor microenvironment.

Язык: Английский

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