Food Chemistry Advances,
Год журнала:
2022,
Номер
1, С. 100069 - 100069
Опубликована: Июль 17, 2022
Mycotoxins
and
food
allergens
are
growing
health
concerns
in
the
area
of
safety.
Accidental
exposure
to
mycotoxins
can
arise
many
ways
including
allergenic
proteins
due
cross-contamination,
unspecified
product
labels
toxic
residues
through
direct
or
indirect
intake
contaminated
food.
In
recent
years,
nanomaterials
various
immunoassays
have
shown
great
potential
selectively
detect
with
lower
detection
limits
a
shorter
time.
This
review
compares
different
immunoassay
approaches,
discusses
applications
carbon
gold
developing
allergens.
Various
types
shapes
carbon,
spherical
non-spherical
nanoparticles
their
synthesis
methods
were
elaborated.
The
approaches
on
how
these
be
incorporated
used
as
combination
improve
performance
addressed.
Lastly,
challenges
prospects
contaminants
discussed.
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2023,
Номер
22(2), С. 1285 - 1311
Опубликована: Янв. 30, 2023
Abstract
Mycotoxin
contamination
in
foods
and
other
goods
has
become
a
broad
issue
owing
to
serious
toxicity,
tremendous
threat
public
safety,
terrible
loss
of
resources.
Herein,
it
is
necessary
develop
simple,
sensitive,
inexpensive,
rapid
platforms
for
the
detection
mycotoxins.
Currently,
limitation
instrumental
chemical
methods
cannot
be
massively
applied
practice.
Immunoassays
are
considered
one
best
candidates
toxin
due
their
simplicity,
rapidness,
cost‐effectiveness.
Especially,
field
dual‐mode
immunosensors
corresponding
assays
rapidly
developing
as
an
advanced
intersected
technology.
So,
this
review
summarized
types
principles
single‐mode
including
optical
electrical
recent
years,
then
focused
on
integrated
combined
detect
mycotoxins,
well
combination
with
portable
device
point‐of‐care
test.
The
remaining
challenges
were
discussed
aim
stimulating
future
development
accelerate
transformation
scientific
laboratory
technologies
into
easy‐to‐operate
platforms.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(3), С. 1916 - 1923
Опубликована: Янв. 9, 2023
Ochratoxin
A
(OTA)
frequently
contaminates
grains
and
consequently
threatens
human
health.
Herein,
we
develop
a
regenerable
signal
probe
apply
it
to
Au-based
screen-printed
electrode
module
(SPE)
for
OTA
determination.
The
probe,
containing
structural
covalent
organic
framework,
gold
nanoparticles
(AuNPs),
indicative
methylene
blue
(MB),
highly
selective
aptamer,
is
synthesized
with
hydrothermal
self-assembly
methods.
SPE
permanently
functionalized
Prussian
(PB),
AuNPs,
semicomplementary
ssDNA.
absorbed
onto
this
via
hybridization,
competitively
expelled
by
OTA,
providing
ratiometric
readout
of
ΔIMB/IPB.
Probe
regeneration,
erase
expired
COF-Au-MB-Apt
after
each
analysis,
established
the
synergy
OTA-conducted
Apt-ssDNA
dissociation
on-chip
thermal
regulation.
This
advantage
powerfully
guarantees
reduplicative
analyses
avoiding
irreversible
Apt-OTA
combination
accumulation
on
sensing
interface.
Regenerations
are
performed
in
repetitive
cycles
(N
=
7)
98.5%
reproduction
efficiency,
IMB
IPB
fluctuations
calculated
as
1.45
1.12%.
method
shows
log-linear
response
wide
range
from
0.2
pg/mL
0.6
μg/mL,
limit
detection
0.12
pg/mL.
During
natural
determinations,
recommended
readouts
match
well
HPLC
less
than
4.82%
relative
error.
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2023,
Номер
23(1)
Опубликована: Дек. 13, 2023
Abstract
Mycotoxin
contamination
of
food
crops
is
a
global
challenge
due
to
their
unpredictable
occurrence
and
severe
adverse
health
effects
on
humans.
Therefore,
it
great
importance
develop
effective
tools
prevent
the
accumulation
mycotoxins
through
chain.
The
use
magnetic
nanoparticle
(MNP)‐assisted
biosensors
for
detecting
mycotoxin
in
complex
foodstuffs
has
garnered
interest
significantly
enhanced
sensitivity
accuracy.
Within
such
context,
this
review
includes
fundamentals
recent
advances
(2020–2023)
area
monitoring
matrices
using
MNP‐based
aptasensors
immunosensors.
In
review,
we
start
by
providing
comprehensive
introduction
design
immunosensors
(natural
antibody
or
nanobody,
random
site‐oriented
immobilization)
(techniques
aptamer
selection,
characterization,
truncation).
Meanwhile,
special
attention
paid
multifunctionalities
MNPs
(recoverable
adsorbent,
versatile
carrier,
signal
indicator)
preparing
mycotoxin‐specific
biosensors.
Further,
contribution
multiplexing
determination
various
summarized.
Finally,
challenges
future
perspectives
practical
applications
MNP‐assisted
are
also
discussed.
progress
updates
shown
expected
offer
readers
valuable
insights
about
detection
applications.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(6), С. 2425 - 2434
Опубликована: Янв. 31, 2024
A
high-throughput,
rapid,
and
highly
sensitive
surface-enhanced
Raman
spectroscopy
(SERS)
microarray
for
screening
multiple
mycotoxins
has
been
developed
on
a
three-dimensional
silver
nanoparticle
porous
silicon
(3D
AgNP-Psi)
SERS
substrate,
which
was
easy
to
be
engineered
by
electrochemical
etching
magnetron
sputtering
technology.
The
current
density,
waveform,
target
material
have
investigated
obtain
an
optimal
3D
substrate.
optimized
AgNP-Psi
substrate
showed
enhancement
factor
of
2.3
×
107
at
400
mA/cm2
constant
density
20
s
Ag
200
nm
thickness
the
surface
Psi.
simulation
electric
field
distribution
near-field
can
reach
3×
higher
than
that
AuNPs.
protein
designed
screen
AuNP
tags
competitive
immunoassay
protocol
displayed
wide
linear
detection
ranges
0.001–100
ng/mL
ochratoxin
A,
0.01–100
aflatoxin
B1,
0.001–10
deoxynivalenol,
along
with
pg/mL
low
limit
detection,
good
recovery
rates,
repeatability,
reproducibility.
is
easily
great
potential
application
in
medicine,
environment,
food
industry
fields.
ACS Omega,
Год журнала:
2024,
Номер
9(8), С. 8754 - 8762
Опубликована: Фев. 13, 2024
The
ubiquitous
aflatoxin
B1
(AFB1)
contamination
in
foods
and
other
complex
matrices
has
brought
great
challenges
for
onsite
monitoring.
In
this
study,
an
ultrasensitive
Nafion-immobilized
functionalized
multiwalled
carbon
nanotube
(MWCNT)-based
electrochemical
(EC)
immunosensor
was
developed
trace
AFB1
detection.
introduced
Nafion
film
could
steadily
stabilize
MWCNTs
with
uniform
distribution
tiling
on
the
surface
of
a
Au
electrode.
Functionalized
large
specific
area,
numerous
active
sites
to
couple
abundant
anti-AFB1
monoclonal
antibodies
(mAbs),
high
conductivity
served
as
signal
amplifier
remarkably
enhancing
sensing
performance
immunosensor.
presence
AFB1,
it
specifically
captured
by
mAbs
reduce
amplified
current
signals,
which
were
recorded
differential
pulse
voltammetry
accurate
quantitation
AFB1.
Because
synergistic
effects
stabilization
enhancers,
EC
exhibited
extremely
selectivity,
excellent
sensitivity
limit
detection
low
0.021
ng/mL,
wide
dynamic
range
0.05–100
besides
fascinating
merits
easy
construction,
cost,
good
stability
7
days,
reusability.
anti-interference
ability
verified
against
three
mycotoxins,
practicability
accuracy
confirmed
measuring
fortified
malt,
lotus
seed,
hirudo
samples
satisfactory
recovery
92.08–104.62%.
This
novel
immunosensing
platform
be
extended
detect
more
mycotoxins
ensure
food
safety.
