Food Chemistry, Год журнала: 2025, Номер unknown, С. 143101 - 143101
Опубликована: Янв. 1, 2025
Язык: Английский
Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2023, Номер 22(2), С. 1285 - 1311
Опубликована: Янв. 30, 2023
Abstract Mycotoxin contamination in foods and other goods has become a broad issue owing to serious toxicity, tremendous threat public safety, terrible loss of resources. Herein, it is necessary develop simple, sensitive, inexpensive, rapid platforms for the detection mycotoxins. Currently, limitation instrumental chemical methods cannot be massively applied practice. Immunoassays are considered one best candidates toxin due their simplicity, rapidness, cost‐effectiveness. Especially, field dual‐mode immunosensors corresponding assays rapidly developing as an advanced intersected technology. So, this review summarized types principles single‐mode including optical electrical recent years, then focused on integrated combined detect mycotoxins, well combination with portable device point‐of‐care test. The remaining challenges were discussed aim stimulating future development accelerate transformation scientific laboratory technologies into easy‐to‐operate platforms.
Язык: Английский
Процитировано
59
Environmental Pollution, Год журнала: 2022, Номер 316, С. 120695 - 120695
Опубликована: Ноя. 21, 2022
Язык: Английский
Процитировано
51
Food Chemistry, Год журнала: 2023, Номер 429, С. 136834 - 136834
Опубликована: Июль 11, 2023
Язык: Английский
Процитировано
43
Trends in Food Science & Technology, Год журнала: 2023, Номер 141, С. 104175 - 104175
Опубликована: Сен. 22, 2023
Язык: Английский
Процитировано
38
Analytical Chemistry, Год журнала: 2023, Номер 95(3), С. 1916 - 1923
Опубликована: Янв. 9, 2023
Ochratoxin A (OTA) frequently contaminates grains and consequently threatens human health. Herein, we develop a regenerable signal probe apply it to Au-based screen-printed electrode module (SPE) for OTA determination. The probe, containing structural covalent organic framework, gold nanoparticles (AuNPs), indicative methylene blue (MB), highly selective aptamer, is synthesized with hydrothermal self-assembly methods. SPE permanently functionalized Prussian (PB), AuNPs, semicomplementary ssDNA. absorbed onto this via hybridization, competitively expelled by OTA, providing ratiometric readout of ΔIMB/IPB. Probe regeneration, erase expired COF-Au-MB-Apt after each analysis, established the synergy OTA-conducted Apt-ssDNA dissociation on-chip thermal regulation. This advantage powerfully guarantees reduplicative analyses avoiding irreversible Apt-OTA combination accumulation on sensing interface. Regenerations are performed in repetitive cycles (N = 7) 98.5% reproduction efficiency, IMB IPB fluctuations calculated as 1.45 1.12%. method shows log-linear response wide range from 0.2 pg/mL 0.6 μg/mL, limit detection 0.12 pg/mL. During natural determinations, recommended readouts match well HPLC less than 4.82% relative error.
Язык: Английский
Процитировано
34
Food Bioscience, Год журнала: 2023, Номер 56, С. 103110 - 103110
Опубликована: Сен. 9, 2023
Язык: Английский
Процитировано
30
Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2023, Номер 23(1)
Опубликована: Дек. 13, 2023
Abstract Mycotoxin contamination of food crops is a global challenge due to their unpredictable occurrence and severe adverse health effects on humans. Therefore, it great importance develop effective tools prevent the accumulation mycotoxins through chain. The use magnetic nanoparticle (MNP)‐assisted biosensors for detecting mycotoxin in complex foodstuffs has garnered interest significantly enhanced sensitivity accuracy. Within such context, this review includes fundamentals recent advances (2020–2023) area monitoring matrices using MNP‐based aptasensors immunosensors. In review, we start by providing comprehensive introduction design immunosensors (natural antibody or nanobody, random site‐oriented immobilization) (techniques aptamer selection, characterization, truncation). Meanwhile, special attention paid multifunctionalities MNPs (recoverable adsorbent, versatile carrier, signal indicator) preparing mycotoxin‐specific biosensors. Further, contribution multiplexing determination various summarized. Finally, challenges future perspectives practical applications MNP‐assisted are also discussed. progress updates shown expected offer readers valuable insights about detection applications.
Язык: Английский
Процитировано
25
Analytical Chemistry, Год журнала: 2024, Номер 96(6), С. 2425 - 2434
Опубликована: Янв. 31, 2024
A high-throughput, rapid, and highly sensitive surface-enhanced Raman spectroscopy (SERS) microarray for screening multiple mycotoxins has been developed on a three-dimensional silver nanoparticle porous silicon (3D AgNP-Psi) SERS substrate, which was easy to be engineered by electrochemical etching magnetron sputtering technology. The current density, waveform, target material have investigated obtain an optimal 3D substrate. optimized AgNP-Psi substrate showed enhancement factor of 2.3 × 107 at 400 mA/cm2 constant density 20 s Ag 200 nm thickness the surface Psi. simulation electric field distribution near-field can reach 3× higher than that AuNPs. protein designed screen AuNP tags competitive immunoassay protocol displayed wide linear detection ranges 0.001–100 ng/mL ochratoxin A, 0.01–100 aflatoxin B1, 0.001–10 deoxynivalenol, along with pg/mL low limit detection, good recovery rates, repeatability, reproducibility. is easily great potential application in medicine, environment, food industry fields.
Язык: Английский
Процитировано
17
ACS Omega, Год журнала: 2024, Номер 9(8), С. 8754 - 8762
Опубликована: Фев. 13, 2024
The ubiquitous aflatoxin B1 (AFB1) contamination in foods and other complex matrices has brought great challenges for onsite monitoring. In this study, an ultrasensitive Nafion-immobilized functionalized multiwalled carbon nanotube (MWCNT)-based electrochemical (EC) immunosensor was developed trace AFB1 detection. introduced Nafion film could steadily stabilize MWCNTs with uniform distribution tiling on the surface of a Au electrode. Functionalized large specific area, numerous active sites to couple abundant anti-AFB1 monoclonal antibodies (mAbs), high conductivity served as signal amplifier remarkably enhancing sensing performance immunosensor. presence AFB1, it specifically captured by mAbs reduce amplified current signals, which were recorded differential pulse voltammetry accurate quantitation AFB1. Because synergistic effects stabilization enhancers, EC exhibited extremely selectivity, excellent sensitivity limit detection low 0.021 ng/mL, wide dynamic range 0.05–100 besides fascinating merits easy construction, cost, good stability 7 days, reusability. anti-interference ability verified against three mycotoxins, practicability accuracy confirmed measuring fortified malt, lotus seed, hirudo samples satisfactory recovery 92.08–104.62%. This novel immunosensing platform be extended detect more mycotoxins ensure food safety.
Язык: Английский
Процитировано
13
Biosensors and Bioelectronics, Год журнала: 2024, Номер 259, С. 116401 - 116401
Опубликована: Май 15, 2024
Язык: Английский
Процитировано
11