MedComm – Biomaterials and Applications,
Год журнала:
2023,
Номер
2(3)
Опубликована: Сен. 1, 2023
Abstract
Nanobodies
(Nbs)
are
the
variable
domain
of
heavy‐chain
antibodies
derived
from
blood
camelids
or
sharks.
smallest
antibody
fragment
with
intact
antigen‐binding
ability.
Compared
to
conventional
antibodies,
nanobodies
have
unique
properties
such
as
small
size,
excellent
stability
and
solubility,
low
immunogenicity,
ability
recognize
hidden
epitopes,
high
tissue
penetration,
industrialized
production.
More
excitingly,
camelid‐specific
amino
acid
sequences
in
framework
mutated
their
human
equivalent,
which
is
humanized,
a
wide
range
applications.
These
superior
characteristics
make
nanobody
an
ideal
alternative
antibody,
showing
prospects
for
various
applications
structural
biology,
molecular
imaging,
disease
diagnosis
therapy,
agricultural
products,
environmental
chemicals
detection.
With
continuous
updating
theories
rapid
development
technology,
screening
expression
methods
increasingly
mature.
Consequently,
several
technologies
identify
express
been
established,
use
cases
described.
In
this
review,
we
summarize
recent
advances
discovery
production
novel
nanobodies,
detection
platforms.
Biosensors,
Год журнала:
2023,
Номер
13(6), С. 640 - 640
Опубликована: Июнь 9, 2023
Viruses
are
widespread
in
the
environment,
and
many
of
them
major
pathogens
serious
plant,
animal,
human
diseases.
The
risk
pathogenicity,
together
with
capacity
for
constant
mutation,
emphasizes
need
measures
to
rapidly
detect
viruses.
highly
sensitive
bioanalytical
methods
diagnose
monitor
socially
significant
viral
diseases
has
increased
past
few
years.
This
is
due,
on
one
hand,
incidence
general
(including
unprecedented
spread
a
new
coronavirus
infection,
SARS-CoV-2),
and,
other
overcome
limitations
modern
biomedical
diagnostic
methods.
Phage
display
technology
antibodies
as
nano-bio-engineered
macromolecules
can
be
used
sensor-based
virus
detection.
review
analyzes
commonly
detection
approaches
shows
prospects
use
prepared
by
phage
sensing
elements
Biosensors,
Год журнала:
2025,
Номер
15(3), С. 183 - 183
Опубликована: Март 13, 2025
Food
safety
remains
a
significant
global
challenge
that
affects
human
health.
Various
hazards,
including
microbiological
and
chemical
threats,
can
compromise
food
throughout
the
supply
chain.
To
address
issues
ensure
public
health,
it
is
necessary
to
adopt
rapid,
accurate,
highly
specific
detection
methods.
Immunoassays
are
considered
be
an
effective
method
for
of
sensitive
biochemical
indicators
provide
efficient
platform
identification
hazards.
In
immunoassays,
antibodies
function
as
primary
recognition
elements.
Nanobodies
have
potential
valuable
biomolecules
in
diagnostic
applications.
Their
distinctive
physicochemical
structural
characteristics
make
them
excellent
candidates
development
reliable
assays,
promising
alternatives
monoclonal
polyclonal
antibodies.
Herein,
we
summarize
comprehensive
overview
status
prospects
nanobody-based
immunoassays
ensuring
safety.
First,
begin
with
historical
perspective
on
nanobodies
their
unique
characteristics.
Subsequently,
explore
definitions
boundaries
immunosensors,
before
discussing
applications
testing
emerged
over
past
five
years,
follow
different
highlighting
advantages
traditional
Finally,
directions
challenges
discussed.
Due
remarkable
sensitivity,
specificity
versatility,
hold
great
promise
revolutionizing
health
well-being.
