Low-density polyethylene enhances the disturbance of microbiome and antibiotic resistance genes transfer in soil-earthworm system induced by pyraclostrobin

Journal of Hazardous Materials, Год журнала: 2024, Номер 465, С. 133459 - 133459

Опубликована: Янв. 8, 2024

Язык: Английский

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Effects of Zinc Thiazole and Oxytetracycline on the Microbial Metabolism, Antibiotic Resistance, and Virulence Factor Genes of Soil, Earthworm Gut, and Phyllosphere

Environmental Science & Technology, Год журнала: 2023, Номер 58(1), С. 160 - 170

Опубликована: Дек. 26, 2023

Pesticides and antibiotics are believed to increase the incidence of antibiotic resistance genes (ARGs) virulence factor (VFGs), constituting a serious threat global health. However, impact this combined pollution on microbiome that related ARGs VFGs soil–plant–animal systems remain unknown. In study, 60-day microcosm experiment was conducted reveal effects zinc thiazole (ZT) oxytetracycline (OTC) microbial communities, resistomes, factors in soil, earthworm gut, phyllosphere samples using metagenomics. ZT exposure perturbed communities nutrient metabolism increased abundance gut. Combined changed profiles by decreasing diversity phyllosphere. Host-tracking analysis identified some genera, such as Citrobacter Aeromonas, frequent hosts Notably, co-occurrence patterns MGEs were observed metagenome-assembled contigs. More importantly, markedly potentially drug-resistant pathogens Acinetobacter soli junii Overall, study expands our current understanding spread under pollutant-induced stress associated health risks.

Язык: Английский

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Lipid Metabolites as Potential Regulators of the Antibiotic Resistome in Tetramorium caespitum

Environmental Science & Technology, Год журнала: 2024, Номер 58(10), С. 4476 - 4486

Опубликована: Фев. 21, 2024

Antibiotic resistance genes (ARGs) are ancient but have become a modern critical threat to health. Gut microbiota, dynamic reservoir for ARGs, transfer between individuals. Surveillance of the antibiotic resistome in gut during different host growth phases is understanding dynamics this ecosystem. Herein, we disentangled ARG profiles and mechanism ARGs egg adult Tetramorium caespitum. Experimental results showed remarkable difference both microbiota with development T. Meta-based metagenomic indicated generalizability development. By using Raman spectroscopy metabolomics, metabolic phenotype metabolites that biotic phase significantly changed lipid metabolism as caespitum aged. Lipid were demonstrated main factor driving enrichment Cuminaldehyde, antibacterial metabolite displayed increase phase, was strongly induce abundance. Our findings show developmental stage-dependent likely modulated by metabolites, offering novel insights into possible steps reduce dissemination soil food chain.

Язык: Английский

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Mechanisms Underlying the Overlooked Chiral Fungicide-Driven Enantioselective Proliferation of Antibiotic Resistance in Earthworm Intestinal Microbiome

Environmental Science & Technology, Год журнала: 2024, Номер 58(6), С. 2931 - 2943

Опубликована: Фев. 2, 2024

From a "One Health" perspective, the global threat of antibiotic resistance genes (ARGs) is associated with modern agriculture practices including agrochemicals application. Chiral fungicides account for considerable proportion wildly used agrochemicals; however, whether and how their enantiomers lead to differential proliferation in agricultural environments remain overlooked. Focused on soil-earthworm ecosystem, we first time deciphered mechanisms underlying enantioselective driven by typical chiral fungicide mandipropamid (i.e., R-MDP S-MDP) utilizing multiomic approach. Time-series metagenomic analysis revealed that led significant enhancement ARGs potential mobility (particularly plasmid-borne ARGs) earthworm intestinal microbiome. We further demonstrated induced concentration-dependent facilitation plasmid-mediated ARG transfer among microbes. In addition, transcriptomic verification identified key aspects involved, where enhanced cell membrane permeability, ability, biofilm formation quorum sensing, rebalanced energy production, decreased versus S-MDP. Overall, findings provide novel insights into disruption microbiome resistome gut offer contributions comprehensive risk assessment agroecosystems.

Язык: Английский

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Photocatalysis Inhibits the Emergence of Multidrug-Resistant Bacteria in an Antibiotic-Resistant Bacterial Community in Aquatic Environments

Environmental Science & Technology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 9, 2024

Bacterial antibiotic resistance has recently attracted increasing amounts of attention. Here, an artificially antibiotic-resistant bacterial community (ARBC) combined with five different constructed bacteria (ARB) single resistance, namely, kanamycin (KAN), tetracycline (TET), cefotaxime (CTX), polymyxin B (PB), or gentamicin (GEM), was studied for the stress response to photocatalysis. With photocatalytic inactivation, transfer and diffusion genes (ARGs) in ARBC decreased, fewer multidrug-resistant (MDRB) emerged aquatic environments. After several days inactivation Luria broth cultivation, >90% ARB were transformed antibiotic-susceptible by discarding ARGs. Bacteria double dominant species (99%) residual ARB. The changes ARG abundance varied, decreasing GEM TET KAN genes. change level consistent abundance. Correspondingly, point mutations occurred KAN, CTX PB after which might be reason why these persisted longer ARBC. In summary, could reduce some ARGs inhibit emergence MDRB as well block This work highlights advantages long-term controlling facilitates a better understanding communities real

Язык: Английский

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Exploring ecological effects of arsenic and cadmium combined exposure on cropland soil: from multilevel organisms to soil functioning by multi-omics coupled with high-throughput quantitative PCR

Journal of Hazardous Materials, Год журнала: 2024, Номер 466, С. 133567 - 133567

Опубликована: Янв. 20, 2024

Язык: Английский

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Antibiotics and Pesticides Enhancing the Transfer of Resistomes among Soil-Bayberry-Fruit Fly Food Chain in the Orchard Ecosystem

Environmental Science & Technology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 4, 2024

While substantial amounts of antibiotics and pesticides are applied to maintain orchard yields, their influence on the dissemination risk antibiotic resisitome in food chain remains poorly understood. In this study, we characterized bacterial fungal communities differentiated both resistance genes (ARGs) virulence factor (VFGs) soil, Chinese bayberry (matured fallen), fruit fly gut, collected from five geographic locations. Our results showed that guts soils exhibit a higher abundance ARGs VFGs compared with fruits. We identified 112 shared 75 VFGs, aminoglycoside adherence being among most abundant. The co-occurrence network revealed some microbes, such as

Язык: Английский

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Understanding the ecological effects of the fungicide difenoconazole on soil and Enchytraeus crypticus gut microbiome

Environmental Pollution, Год журнала: 2023, Номер 326, С. 121518 - 121518

Опубликована: Март 27, 2023

Язык: Английский

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Parental exposure to Cypermethrin causes intergenerational toxicity in zebrafish offspring

The Science of The Total Environment, Год журнала: 2024, Номер 935, С. 173456 - 173456

Опубликована: Май 22, 2024

Язык: Английский

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Insights into the mobility and bacterial hosts of antibiotic resistance genes under dinotefuran selection pressure in aerobic granular sludge based on metagenomic binning and functional modules

Environmental Research, Год журнала: 2025, Номер 268, С. 120807 - 120807

Опубликована: Янв. 9, 2025

Язык: Английский

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