Опубликована: Янв. 1, 2023

Electrokinetic-enhanced bioremediation (EK-Bio) has been developed as a promising technology for the remediation of subsurface trichloroethylene (TCE) contamination. The effects produced pure and abundant H2 O2 by anode cathode EK-Bio on microbial community changes TCE biodegradation remain unknown. This study investigated influence high dissolved (DH, 0.2-0.7 mg/L) (DO, 2.6-6.6 concentrations transformation indigenous communities in TCE-contaminated sediment, including TCE-removing populations, using 16S rRNA sequencing, functional gene analyses. results showed that introduction enhanced degradation, causing 90.4% removal four weeks, cis-dichloroethylene (cis-DCE) was accordingly. following three ways should be responsible this promoted degradation. Firstly, DH rapidly reduced oxidation-reduction potential (ORP) value to around -500 mV, beneficial dechlorination. Secondly, significantly changed enrichment anaerobic dechlorinators, such Sulfuricurvum, Sulfurospirillum, Geobacter, Desulfitobacterium, increased abundance dechlorination pceA. Thirdly, environment alleviated electronic competition between dechlorinators sulfate-reducing bacteria. However, bio-remediation did not occur DO even after ten weeks due decreases aerobic co-metabolic microbes Pseudomonas Comamonas shortage growth substrate mmoX carrier Methyloversatilis. competitive organic carbon (DOC) consumption unfriendly microbe-microbe interactions also interpreted non-degradation environment. These provided evidence mechanism EK-Bio. Providing obligate metabolic bacteria electrochemical cathodes anodes, respectively, or substrates can feasible methods promote shallow aquifer under technology.

Язык: Английский