Electrokinetic-enhanced
bioremediation
(EK-Bio)
has
been
developed
as
a
promising
technology
for
the
remediation
of
subsurface
trichloroethylene
(TCE)
contamination.
The
effects
produced
pure
and
abundant
H2
O2
by
anode
cathode
EK-Bio
on
microbial
community
changes
TCE
biodegradation
remain
unknown.
This
study
investigated
influence
high
dissolved
(DH,
0.2-0.7
mg/L)
(DO,
2.6-6.6
concentrations
transformation
indigenous
communities
in
TCE-contaminated
sediment,
including
TCE-removing
populations,
using
16S
rRNA
sequencing,
functional
gene
analyses.
results
showed
that
introduction
enhanced
degradation,
causing
90.4%
removal
four
weeks,
cis-dichloroethylene
(cis-DCE)
was
accordingly.
following
three
ways
should
be
responsible
this
promoted
degradation.
Firstly,
DH
rapidly
reduced
oxidation-reduction
potential
(ORP)
value
to
around
-500
mV,
beneficial
dechlorination.
Secondly,
significantly
changed
enrichment
anaerobic
dechlorinators,
such
Sulfuricurvum,
Sulfurospirillum,
Geobacter,
Desulfitobacterium,
increased
abundance
dechlorination
pceA.
Thirdly,
environment
alleviated
electronic
competition
between
dechlorinators
sulfate-reducing
bacteria.
However,
bio-remediation
did
not
occur
DO
even
after
ten
weeks
due
decreases
aerobic
co-metabolic
microbes
Pseudomonas
Comamonas
shortage
growth
substrate
mmoX
carrier
Methyloversatilis.
competitive
organic
carbon
(DOC)
consumption
unfriendly
microbe-microbe
interactions
also
interpreted
non-degradation
environment.
These
provided
evidence
mechanism
EK-Bio.
Providing
obligate
metabolic
bacteria
electrochemical
cathodes
anodes,
respectively,
or
substrates
can
feasible
methods
promote
shallow
aquifer
under
technology.
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Июль 30, 2024
Halogenated
organic
compounds
are
persistent
pollutants
that
pose
a
serious
threat
to
human
health
and
the
safety
of
ecosystems.
Cobamides
essential
cofactors
for
reductive
dehalogenases
(RDase)
in
organohalide-respiring
bacteria
(OHRB),
which
catalyze
dehalogenation
process.
This
review
systematically
summarizes
impact
cobamides
on
organohalide
respiration.
The
catalytic
processes
cobamide
also
discussed.
Additionally,
we
examine
OHRB,
cannot
synthesize
must
obtain
it
from
environment
through
salvage
pathway;
co-culture
with
producer
is
more
beneficial
possible.
aims
help
readers
better
understand
importance
function
dehalogenation.
presented
information
can
aid
development
bioremediation
strategies.
Water,
Год журнала:
2024,
Номер
16(21), С. 3154 - 3154
Опубликована: Ноя. 4, 2024
The
present
study
focuses
on
a
site
contaminated
with
halogenated
hydrocarbons,
utilizing
detailed
inventory
of
contamination
data
to
achieve
the
precise
characterization
groundwater
pollution.
Employing
MOFLOW-2000
software,
flow
model
was
established
for
area.
In
conjunction
MT3DMS,
predictive
constructed
simulate
and
forecast
spatiotemporal
distribution
contaminant
migration
attenuation
following
remediation.
simulation
area
delineated
based
geographical
features,
vertical
range
strata
also
determined.
To
establish
hydrogeological
conceptual
target
remediation
site,
comprehensive
were
collected,
encompassing
geological
structures,
hydrological
parameters,
rainfall
information.
Model
calibration
six
layers
low-permeability
aquifer
intervals
revealed
by
exploration
wells
MW1–5,
as
well
groundwater-level
contours
data.
Based
from
September
2010,
an
initial
three-dimensional
tetrachloroethylene
(PCE)
generated.
Subsequently,
solute
transport
PCE
established,
incorporating
various
enhanced
reductive
dechlorination
(ERD)
strategies
applied
at
different
times
locations.
Calibration
against
actual
monitoring
presence
unmonitored
dense
non-aqueous
phase
liquids
(DNAPLs)
contributing
continuous
release
elevation
concentrations.
By
accounting
DNAPL
release,
calibrated
closely
matched
observed
concentration
decay
patterns,
effectively
capturing
dynamics
within
system.
modeling
approach
proposed
in
this
provides
important
support
current
it
is
applicable
simulating
predicting
pollution
scenarios
similar
sites.
Electrokinetic-enhanced
bioremediation
(EK-Bio)
has
been
developed
as
a
promising
technology
for
the
remediation
of
subsurface
trichloroethylene
(TCE)
contamination.
The
effects
produced
pure
and
abundant
H2
O2
by
anode
cathode
EK-Bio
on
microbial
community
changes
TCE
biodegradation
remain
unknown.
This
study
investigated
influence
high
dissolved
(DH,
0.2-0.7
mg/L)
(DO,
2.6-6.6
concentrations
transformation
indigenous
communities
in
TCE-contaminated
sediment,
including
TCE-removing
populations,
using
16S
rRNA
sequencing,
functional
gene
analyses.
results
showed
that
introduction
enhanced
degradation,
causing
90.4%
removal
four
weeks,
cis-dichloroethylene
(cis-DCE)
was
accordingly.
following
three
ways
should
be
responsible
this
promoted
degradation.
Firstly,
DH
rapidly
reduced
oxidation-reduction
potential
(ORP)
value
to
around
-500
mV,
beneficial
dechlorination.
Secondly,
significantly
changed
enrichment
anaerobic
dechlorinators,
such
Sulfuricurvum,
Sulfurospirillum,
Geobacter,
Desulfitobacterium,
increased
abundance
dechlorination
pceA.
Thirdly,
environment
alleviated
electronic
competition
between
dechlorinators
sulfate-reducing
bacteria.
However,
bio-remediation
did
not
occur
DO
even
after
ten
weeks
due
decreases
aerobic
co-metabolic
microbes
Pseudomonas
Comamonas
shortage
growth
substrate
mmoX
carrier
Methyloversatilis.
competitive
organic
carbon
(DOC)
consumption
unfriendly
microbe-microbe
interactions
also
interpreted
non-degradation
environment.
These
provided
evidence
mechanism
EK-Bio.
Providing
obligate
metabolic
bacteria
electrochemical
cathodes
anodes,
respectively,
or
substrates
can
feasible
methods
promote
shallow
aquifer
under
technology.
