Increasing pesticide diversity impairs soil microbial functions

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(2)

Опубликована: Янв. 9, 2025

Pesticide application is essential for stabilizing agricultural production. However, the effects of increasing pesticide diversity on soil microbial functions remain unclear, particularly under varying nitrogen (N) fertilizer management practices. In this study, we investigated stochasticity microbes and multitrophic networks through amplicon sequencing, assessed community related to carbon (C), N, phosphorus (P), sulfur (S) cycling, characterized dominant bacterial life history strategies via metagenomics along a gradient two N addition levels. Our findings show that higher enriches abundance specialists opportunists capable degrading or resisting pesticides, reducing proportion generalists in absence addition. These shifts can complicate networks. Under increased diversity, selective pressure may drive bacteria streamline their average genome size conserve energy while enhancing C, P, S metabolic capacities, thus accelerating nutrient loss. comparison, was found reduce niche differentiation at mitigating impacts network complexity functional traits associated with ultimately alleviating results reveal contrasting different input scenarios emphasize strategic mitigate ecological use systems.

Язык: Английский

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A risk entropy approach for linking pesticides and soil bacterial communities

Journal of Hazardous Materials, Год журнала: 2024, Номер 469, С. 133970 - 133970

Опубликована: Март 5, 2024

Язык: Английский

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Metagenomic analysis reveals the effects of potassium ferrate and steel slag on fate of ARGs in anaerobic sludge digestion system

Journal of Environmental Management, Год журнала: 2025, Номер 373, С. 123981 - 123981

Опубликована: Янв. 1, 2025

Язык: Английский

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Soil warming increases the active antibiotic resistome in the gut of invasive giant African snails

Microbiome, Год журнала: 2025, Номер 13(1)

Опубликована: Фев. 6, 2025

Global warming is redrawing the map for invasive species, spotlighting globally harmful giant African snail as a major ecological disruptor and public health threat. Known harboring extensive antibiotic resistance genes (ARGs) human pathogens, it remains uncertain whether global exacerbates these associated risks. We use phenotype-based single-cell Raman with D2O labeling (Raman-D2O) genotype-based metagenomic sequencing to investigate soil increases active antibiotic-resistant bacteria (ARBs) in gut microbiome of snails. show significant increase beta-lactam phenotypic ARBs rising temperatures, mirrored by surge beta-lactamase such SHV, TEM, OCH, OKP, LEN subtypes. Through correlation analysis between abundance phenotypically genotypically ARG-carrying microbes, we identify species that contribute increased activity resistome under warming. Among 299 high-quality metagenome-assembled genomes (MAGs), further revealed enhances "supercarriers" including pathogens multiple ARGs virulence factors. Furthermore, identified elevated biosynthetic gene clusters (BGCs) within MAGs, third encoding at least one BGC. This suggests link secondary metabolism, enhancing environmental adaptability competitive advantage organisms warmer environments. The study underscores complex interactions snail, highlighting potential escalation risks due These findings emphasize urgent need integrated strategies manage threat context climate change.

Язык: Английский

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Mining strategies for isolating plastic-degrading microorganisms

Environmental Pollution, Год журнала: 2024, Номер 346, С. 123572 - 123572

Опубликована: Фев. 16, 2024

Язык: Английский

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Antibiotics and Pesticides Enhancing the Transfer of Resistomes among Soil-Bayberry-Fruit Fly Food Chain in the Orchard Ecosystem

Environmental Science & Technology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 4, 2024

While substantial amounts of antibiotics and pesticides are applied to maintain orchard yields, their influence on the dissemination risk antibiotic resisitome in food chain remains poorly understood. In this study, we characterized bacterial fungal communities differentiated both resistance genes (ARGs) virulence factor (VFGs) soil, Chinese bayberry (matured fallen), fruit fly gut, collected from five geographic locations. Our results showed that guts soils exhibit a higher abundance ARGs VFGs compared with fruits. We identified 112 shared 75 VFGs, aminoglycoside adherence being among most abundant. The co-occurrence network revealed some microbes, such as

Язык: Английский

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Removal of intracellular and extracellular antibiotic resistance genes and virulence factor genes using electricity-intensified constructed wetlands

Journal of Hazardous Materials, Год журнала: 2024, Номер 475, С. 134749 - 134749

Опубликована: Май 28, 2024

Язык: Английский

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Quaternary ammonium biocides promote conjugative transfer of antibiotic resistance gene in structure- and species-dependent manner

Environment International, Год журнала: 2024, Номер 189, С. 108812 - 108812

Опубликована: Июнь 12, 2024

The linkage between biocides and antibiotic resistance has been widely suggested in laboratories various environments. However, the action mechanism of on genes (ARGs) spread is still unclear. Thus, 6 quaternary ammonium (QACs) with different bonded substituents or alkyl chain lengths were selected to assess their effects conjugation transfer ARGs this study. Two models same donor (E. coli DH5α (RP4)) into two receptors, E. MG1655 pathogenic S. sonnei SE6-1, constructed. All QACs found significantly promote intra- inter-genus conjugative ARGs, frequency was highly impacted by structure receptors. At environmental exposure level (4 × 10

Язык: Английский

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Comparison of Heavy Metal and Disinfectant Resistance of <i>S. aureus</i> and <i>Enterococcus</i> Isolates with Antibiotic Resistance Profiles

OBM Genetics, Год журнала: 2025, Номер 09(01), С. 1 - 12

Опубликована: Янв. 5, 2025

Antimicrobial resistance is one of the most significant threats to our present and future. Recently, it has been suggested that antibiotic-resistant microorganisms also exhibit heavy metals disinfectants, these profiles may interact with each other. Microorganisms can be exposed disinfectants in different ways various environments. They are especially likely quaternary ammonium compounds used food industry or due tap water. Thus, adapt their environment gain thanks horizontal gene transfer. In research, metal disinfectant 42 isolates whose antibiotic were determined previous studies revealed connection between - resistances isolates. The was by determining minimum inhibition concentrations (MIC) using broth microdilution method. MIC values obtained from compared those control well, determined. research found highly resistant cobalt (83%), nickel (86%), triclosan (81%). It observed they utterly zinc lead. Based on data study, thought high rate related resistance. However, more comprehensive needed understand this relationship fully.

Язык: Английский

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The combination of microplastics and glyphosate affects the microbiome of soil inhabitant Enchytraeus crypticus

Journal of Hazardous Materials, Год журнала: 2025, Номер 489, С. 137676 - 137676

Опубликована: Фев. 19, 2025

Язык: Английский

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