Engineering the phycosphere: fundamental concepts and tools for the bottom-up design of microalgal-bacterial consortia DOI Creative Commons

Austin Semple,

Jagroop Pandhal

Applied Phycology, Год журнала: 2024, Номер 6(1), С. 21 - 51

Опубликована: Дек. 23, 2024

Microalgae are a promising platform for the sustainable production of high-value compounds, while also serving as robust degraders toxic pollutants that accumulate in industrial waste streams. As synthetic biologists continue to optimize microalgae so they can compete with conventional microbial hosts, major limitation is compromised central metabolism, following transformation heterologous pathways interfere distribution cellular resources. The co-cultivation microorganisms, including bacteria, has emerged branch biology, rationale distributing metabolic burden inflicted by degradation and synthesis processes aid alleviating stress, polycultures reduce susceptibility contamination. Furthermore, bacteria have co-evolved share resources many aquatic ecosystems, playing pivotal role nutrient cycling. Recent studies these symbioses revealed exchange diverse growth-promoting probiotics, ranging from vitamins metals act co-substrates, amino acids small metabolite infochemicals which dictate population-level shifts phenotype. interface interactions termed phycosphere, dynamic hotspot symbiotic relationships, transient nature, highlighting difficulty establishing stable consortia fit use. Therefore, development interaction screens assays identifying strains optimizing culture conditions important suited desired functions. Equally application meta-omics tools decipher functional interactions, revealing targets improving functionality stability. Here, we review current understanding microalgal microbiome, well bottom-up alga-bacteria co-culture design. We finish perspectives on potential engineering phycosphere applications, where there an increasing need methods manufacturing using adopting circular economy.

Язык: Английский

Engineering the phycosphere: fundamental concepts and tools for the bottom-up design of microalgal-bacterial consortia DOI Creative Commons

Austin Semple,

Jagroop Pandhal

Applied Phycology, Год журнала: 2024, Номер 6(1), С. 21 - 51

Опубликована: Дек. 23, 2024

Microalgae are a promising platform for the sustainable production of high-value compounds, while also serving as robust degraders toxic pollutants that accumulate in industrial waste streams. As synthetic biologists continue to optimize microalgae so they can compete with conventional microbial hosts, major limitation is compromised central metabolism, following transformation heterologous pathways interfere distribution cellular resources. The co-cultivation microorganisms, including bacteria, has emerged branch biology, rationale distributing metabolic burden inflicted by degradation and synthesis processes aid alleviating stress, polycultures reduce susceptibility contamination. Furthermore, bacteria have co-evolved share resources many aquatic ecosystems, playing pivotal role nutrient cycling. Recent studies these symbioses revealed exchange diverse growth-promoting probiotics, ranging from vitamins metals act co-substrates, amino acids small metabolite infochemicals which dictate population-level shifts phenotype. interface interactions termed phycosphere, dynamic hotspot symbiotic relationships, transient nature, highlighting difficulty establishing stable consortia fit use. Therefore, development interaction screens assays identifying strains optimizing culture conditions important suited desired functions. Equally application meta-omics tools decipher functional interactions, revealing targets improving functionality stability. Here, we review current understanding microalgal microbiome, well bottom-up alga-bacteria co-culture design. We finish perspectives on potential engineering phycosphere applications, where there an increasing need methods manufacturing using adopting circular economy.

Язык: Английский

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