Antimicrobial Resistance in the Terrestrial Environment of Agricultural Landscapes in Norway DOI Creative Commons

Live L. Nesse,

Kristin Forfang, Jannice Schau Slettemeås

и другие.

Microorganisms, Год журнала: 2024, Номер 12(9), С. 1854 - 1854

Опубликована: Сен. 6, 2024

The abundance and diversity of antimicrobial-resistant bacteria (ARB) antimicrobial resistance genes (ARGs) in agricultural landscapes may be important for the spread (AMR) environment. aim this study was to apply screening methods ARB ARGs investigate impact farming on prevalence AMR a country with low antibiotic usage. We have analyzed samples (n = 644) from soil wild terrestrial animals plants (slugs, snails, mice, shrews, earthworms, red clover) collected over two years fields accompanied by nearby control areas human activity. All were investigated occurrence 35 different using high-throughput quantitative PCR (HT-qPCR) newly developed DNA array. In addition, first year 415) culture-based approach combined whole-genome sequencing (WGS) identify E. coli (AREC). detected 59.5% all (2019 + 2020). AREC, which only 2019 samples, identified 1.9% these. Samples autumn showed more AREC than spring pronounced organic conventional fields. Control activity lower levels lack AREC. use livestock manure correlated higher level ARG load other practices. None contained antibiotics, no association found between metals or pesticides. High qualitative similarity HT-qPCR WGS, together positive controls validation our assays, show that microfluid array an efficient tool environmental samples. conclusion, even very consumption antimicrobials production animals, results support hypothesis these being source environments, primarily through manure.

Язык: Английский

Whole genome sequencing revealed high occurrence of antimicrobial resistance genes in bacteria isolated from poultry manure DOI
Animesh Tripathi,

Anjali Jaiswal,

Dinesh Kumar

и другие.

International Journal of Antimicrobial Agents, Год журнала: 2025, Номер unknown, С. 107452 - 107452

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Tracking virulence factor genes and their combination with resistance genes for microbial risk assessment in drinking water treatment plants DOI

Qingqing Zhao,

Xifen Pan,

Tingyue Gu

и другие.

Journal of Water Process Engineering, Год журнала: 2025, Номер 73, С. 107741 - 107741

Опубликована: Апрель 16, 2025

Язык: Английский

Процитировано

0

Characterization of antibiotic resistance genes and virulence factors in organic managed tea plantation soils in southwestern China by metagenomics DOI Creative Commons

Taobing Yu,

Lang Cheng,

Qing Zhang

и другие.

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Май 1, 2025

Sustainable organic management practices have gained significant attentions for its potential health and environmental benefits. However, the spread of antibiotic resistance genes (ARGs) virulence factors (VFs) in soils, plants, agricultural products has severely limited development managements on agriculture. At present, distribution assembly ARGs VFs managed tea plantation systems remains largely unknown. Here, we used metagenomic analysis to explore soil microbial taxa, 20 years conventional (CM) (OM) soils. Results showed that total abundance OM was 16.9% (p < 0.001) higher than CM, increased were rpoB2, evgS, MuxB, TaeA, efrA. As VFs, significantly adherence, stress protein actin-based motility compared CM. Moreover, relative taxa harboring which Streptomyces, Pseudomonas, Terrabacter, Network suggested positive interactions taxa-ARGs, taxa-VFs ARGs-VFs Impact stochastic process stronger Overall, these findings provide a basis integrating ARGs, pathogen hosts assess ecological risks long-term efforts need be done reducing bacterial pathogens fertilizers

Язык: Английский

Процитировано

0

Soil Microbial Adaptation and Biogeochemical Feedback in Degraded Alpine Meadows of the Qinghai–Tibetan Plateau DOI Creative Commons

Bingzhang Li,

Quzhen Gesang,

Yan Sun

и другие.

Microorganisms, Год журнала: 2025, Номер 13(5), С. 1142 - 1142

Опубликована: Май 16, 2025

Alpine meadows on the Qinghai-Tibetan Plateau are experiencing rapid degradation due to climate change and anthropogenic disturbances, leading severe ecological consequences. In this study, we investigated response of soil microbial communities their metabolic functions across a gradient using metagenomic sequencing comprehensive physicochemical analysis in city Lhasa, China. Results showed that pH increased with degradation, while most nutrients, including different forms nitrogen, phosphorus, potassium, declined. pH, ammonium organic matter were identified as key factors driving dynamics. Microbial community composition shifted markedly, distinct biomarker taxa emerging at levels. Network revealed progressive loss connectivity, Actinobacteria dominance increasing heavily degraded soils, cross-phylum interactions weakened. Functional biogeochemical cycling genes carbon, phosphorus all disrupted by but each exhibited unique patterns. These findings will extend our understanding microbial-mediated processes under provide scientific foundation for ecosystem management, conservation, targeted restoration strategies alpine meadows.

Язык: Английский

Процитировано

0

Unveiling potential roles of earthworms in mitigating the presence of virulence factor genes in terrestrial ecosystems DOI
Shu-Ting Xie, Dong Zhu,

Ya-Qiong Song

и другие.

Journal of Hazardous Materials, Год журнала: 2024, Номер 476, С. 135133 - 135133

Опубликована: Июль 8, 2024

Язык: Английский

Процитировано

2

Metagenomics reveals the characteristics and potential spread of microbiomes and virulence factor genes in the dairy cattle production system DOI

Yimin Zhuang,

Shuai Liu, Jianxin Xiao

и другие.

Journal of Hazardous Materials, Год журнала: 2024, Номер 480, С. 136005 - 136005

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

1

Deciphering the mechanism of rhizosphere microecosystem in modulating rice cadmium accumulation via integrating metabolomics and metagenomics DOI
Mengmeng Wang,

Jiayin Zhao,

Yongjing Gu

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 959, С. 178181 - 178181

Опубликована: Дек. 26, 2024

Язык: Английский

Процитировано

1

Microbial nitrogen transformation regulates pathogenic virulence in soil environment DOI

Xiujing Guan,

Yan Xu, Dandan Zhang

и другие.

Journal of Environmental Management, Год журнала: 2024, Номер 369, С. 122280 - 122280

Опубликована: Сен. 2, 2024

Язык: Английский

Процитировано

1

Antimicrobial Resistance in the Terrestrial Environment of Agricultural Landscapes in Norway DOI Creative Commons

Live L. Nesse,

Kristin Forfang, Jannice Schau Slettemeås

и другие.

Microorganisms, Год журнала: 2024, Номер 12(9), С. 1854 - 1854

Опубликована: Сен. 6, 2024

The abundance and diversity of antimicrobial-resistant bacteria (ARB) antimicrobial resistance genes (ARGs) in agricultural landscapes may be important for the spread (AMR) environment. aim this study was to apply screening methods ARB ARGs investigate impact farming on prevalence AMR a country with low antibiotic usage. We have analyzed samples (n = 644) from soil wild terrestrial animals plants (slugs, snails, mice, shrews, earthworms, red clover) collected over two years fields accompanied by nearby control areas human activity. All were investigated occurrence 35 different using high-throughput quantitative PCR (HT-qPCR) newly developed DNA array. In addition, first year 415) culture-based approach combined whole-genome sequencing (WGS) identify E. coli (AREC). detected 59.5% all (2019 + 2020). AREC, which only 2019 samples, identified 1.9% these. Samples autumn showed more AREC than spring pronounced organic conventional fields. Control activity lower levels lack AREC. use livestock manure correlated higher level ARG load other practices. None contained antibiotics, no association found between metals or pesticides. High qualitative similarity HT-qPCR WGS, together positive controls validation our assays, show that microfluid array an efficient tool environmental samples. conclusion, even very consumption antimicrobials production animals, results support hypothesis these being source environments, primarily through manure.

Язык: Английский

Процитировано

0