Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile acid pathway DOI Creative Commons
Jian Chen, Kanwar Kumar Malhi, Xiaowei Li

и другие.

Animal nutrition, Год журнала: 2024, Номер 20, С. 95 - 109

Опубликована: Ноя. 2, 2024

Weaning stress causes substantial economic loss in the swine industry. Moreover, weaning-induced intestinal barrier damage and dysfunction of gut-liver axis are associated with reduced growth performance piglets. Metasilicate-based alkaline mineral water (AMW) has shown potential therapeutic effects on gastrointestinal disorders; however, mechanisms involved their overall have not been explored. Here, sodium metasilicate (SMS) was used to prepare metasilicate-based AMW (basal + 500 mg/L SMS). A total 240 newly weaned piglets were allocated Control SMS groups (6 replicate pens per group 20 pen) for a 15-day trial period. Histopathological evaluations conducted using hematoxylin eosin staining. To analyze composition gut microbiota, 16S rRNA PacBio SMRT Gene Full-Length Sequencing performed. Western blotting immunofluorescence employed assess protein expression levels. Our results indicated that effectively alleviated colonic or liver morphological injury inflammatory response, as well cholesterol metabolism disorders. Further analysis showed promoted deoxycholic acid (DCA) biosynthesis by increasing abundance Lactobacillus_delbrueckii colon (P < 0.001). This consequently improved through DCA-secondary bile (SBA) receptors (SBAR)-nuclear factor-kappaB (NF-κB)/NOD-like receptor family pyrin domain-containing 3 (NLRP3) pathways. Growth parameters, including final body weight = 0.034) average daily gain 0.001), significantly higher than those group. Therefore, maintains homeostasis regulating microbiota-mediated SBA-SBAR pathway under weaning stress. research provides new strategy mitigating stress-induced

Язык: Английский

A New Insight into the Mechanism of Atrazine-Induced Neurotoxicity: Triggering Neural Stem Cell Senescence by Activating Integrated Stress Response Pathway DOI Creative Commons
Jian Chen, Xueyan Dai, Kanwar Kumar Malhi

и другие.

Research, Год журнала: 2024, Номер 7

Опубликована: Янв. 1, 2024

Atrazine (AT), a widely utilized chemical herbicide, causes widespread contamination of agricultural water bodies. Recently, exposure to AT has been linked the development age-related neurodegenerative diseases (NDs), suggesting its neurotoxicity potential. As an endocrine disruptor, targets hypothalamus, crucial part neuroendocrine system. However, toxicological mechanism hypothalamus and correlation with ND remain unexplored. Our results indicated that caused significant morphological structural damage leading loss mature intact neurons microglial activation. Furthermore, hypothalamic neural stem cells (HtNSCs) were recruited areas neuronal by AT. Through in vivo vitro experiments, we clarified outcomes AT-induced HtNSC recruitment alongside mature/intact neurons. Mechanistically, induces senescence these HtNSCs activating integrated stress response signaling. This consequently hinders repair damaged inhibiting proliferation differentiation. Overall, our findings underscore pivotal role pathway damage. Additionally, present study offers novel perspectives understand mechanisms provides preliminary evidence linking NDs.

Язык: Английский

Процитировано

1
Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile acid pathway DOI Creative Commons
Jian Chen, Kanwar Kumar Malhi, Xiaowei Li

и другие.

Animal nutrition, Год журнала: 2024, Номер 20, С. 95 - 109

Опубликована: Ноя. 2, 2024

Weaning stress causes substantial economic loss in the swine industry. Moreover, weaning-induced intestinal barrier damage and dysfunction of gut-liver axis are associated with reduced growth performance piglets. Metasilicate-based alkaline mineral water (AMW) has shown potential therapeutic effects on gastrointestinal disorders; however, mechanisms involved their overall have not been explored. Here, sodium metasilicate (SMS) was used to prepare metasilicate-based AMW (basal + 500 mg/L SMS). A total 240 newly weaned piglets were allocated Control SMS groups (6 replicate pens per group 20 pen) for a 15-day trial period. Histopathological evaluations conducted using hematoxylin eosin staining. To analyze composition gut microbiota, 16S rRNA PacBio SMRT Gene Full-Length Sequencing performed. Western blotting immunofluorescence employed assess protein expression levels. Our results indicated that effectively alleviated colonic or liver morphological injury inflammatory response, as well cholesterol metabolism disorders. Further analysis showed promoted deoxycholic acid (DCA) biosynthesis by increasing abundance Lactobacillus_delbrueckii colon (P < 0.001). This consequently improved through DCA-secondary bile (SBA) receptors (SBAR)-nuclear factor-kappaB (NF-κB)/NOD-like receptor family pyrin domain-containing 3 (NLRP3) pathways. Growth parameters, including final body weight = 0.034) average daily gain 0.001), significantly higher than those group. Therefore, maintains homeostasis regulating microbiota-mediated SBA-SBAR pathway under weaning stress. research provides new strategy mitigating stress-induced

Язык: Английский

Процитировано

0