Discovery of two novel cutinases from a gut yeast of plastic-eating mealworm for polyester depolymerization DOI Creative Commons

Huang Tong,

Jingya Zhang,

Xuena Dong

и другие.

Applied and Environmental Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 2, 2025

Identification of novel plastic-degrading enzymes is crucial for developing enzymatic degradation and recycling strategies plastic waste. Here, we report the discovery two cutinases, SiCut1 SiCut2, from a yeast strain Sakaguchia sp. BIT-D3 was isolated gut plastic-eating mealworms. Their amino acid sequences share less than 25% identity with all previously described cutinases reveal conserved S-D-H catalytic triad unique GYSKG motif. recombinant proteins were successfully overexpressed in Pichia pastoris. The pH range both 4.0 to 11.0 temperature SiCut2 10°C 50°C 70°C, respectively. Both showed strong activity against apple cutin short-chain fatty esters p-nitrophenol glycerol, substantiating their classification as true cutinases. have been demonstrated exhibit efficient polycaprolactone (PCL) film, polybutylene succinate (PBS) polyester-polyurethane (PUR) foam. Molecular docking molecular dynamics simulations used elucidate underlying mechanisms observed thermal stability. This study shows that are yeast-derived potential depolymerization waste.IMPORTANCEThe identification critical addressing pervasive problem pollution. presents derived plastic-feeding Despite sharing sequence known remarkable capabilities various polyester plastics, including Our results provide insights into applications strategies. By harnessing microbiota organisms, this research lays foundation innovative enzyme-based solutions reduce waste promote sustainable practices management.

Язык: Английский

Purification and molecular docking of α-glucosidase inhibitory peptides from mung bean protein hydrolysates DOI Creative Commons
Lina Li,

Jingru Sun,

Zhao Wanting

и другие.

LWT, Год журнала: 2025, Номер unknown, С. 117545 - 117545

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Discovery of two novel cutinases from a gut yeast of plastic-eating mealworm for polyester depolymerization DOI Creative Commons

Huang Tong,

Jingya Zhang,

Xuena Dong

и другие.

Applied and Environmental Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 2, 2025

Identification of novel plastic-degrading enzymes is crucial for developing enzymatic degradation and recycling strategies plastic waste. Here, we report the discovery two cutinases, SiCut1 SiCut2, from a yeast strain Sakaguchia sp. BIT-D3 was isolated gut plastic-eating mealworms. Their amino acid sequences share less than 25% identity with all previously described cutinases reveal conserved S-D-H catalytic triad unique GYSKG motif. recombinant proteins were successfully overexpressed in Pichia pastoris. The pH range both 4.0 to 11.0 temperature SiCut2 10°C 50°C 70°C, respectively. Both showed strong activity against apple cutin short-chain fatty esters p-nitrophenol glycerol, substantiating their classification as true cutinases. have been demonstrated exhibit efficient polycaprolactone (PCL) film, polybutylene succinate (PBS) polyester-polyurethane (PUR) foam. Molecular docking molecular dynamics simulations used elucidate underlying mechanisms observed thermal stability. This study shows that are yeast-derived potential depolymerization waste.IMPORTANCEThe identification critical addressing pervasive problem pollution. presents derived plastic-feeding Despite sharing sequence known remarkable capabilities various polyester plastics, including Our results provide insights into applications strategies. By harnessing microbiota organisms, this research lays foundation innovative enzyme-based solutions reduce waste promote sustainable practices management.

Язык: Английский

Процитировано

0