Multi-locus genome-wide association mapping for major agronomic and yield-related traits in sorghum (Sorghum bicolor (L.) moench) landraces DOI Creative Commons

Addisu Getahun,

Admas Alemu,

Habte Nida

и другие.

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Март 28, 2025

Sorghum is a vital cereal crop for over 750 million people, ranking 5th globally. It has multiple purposes, including food, feed, and biofuels, essential in Ethiopia, which rich genetic diversity of various agroecological zones. Explore marker-trait associations (MTAs) to identify quantitative trait nucleotides (QTNs) new candidate genes associated with agronomic yield contributing traits Ethiopian sorghum landraces using multi-locus GWAS models assist the genomic-assisted breeding strategies. This study investigates basis through Genome-Wide Association Studies (ML-GWAS). 216 landraces, improved varieties, check cultivars were obtained from Biodiversity Institute National Improvement Program this study. The experiment was conducted two cropping seasons, employing an α-lattice design phenotyping key such as days flowering, maturity, plant height, seed number per plant, grain yield, thousand weight. A mixed linear model (MLM) used analyze phenotypic data estimate parameters variances broad sense heritability. GBS ApeKI restriction enzyme provided 50,165 high-quality SNP markers. six ML-GWAS identified significant QTNs LOD score threshold value ≥ 4.0. analysis revealed major across chromosomes, supported by stringent filtering criterion that ensured reliability. Co-localization known QTLs explored QTL Atlas database within QTN regions, providing architecture influencing performance via Phytozome platform biomaRt package. Pearson correlation among most traits, p-values less than 0.0001, except showed lower correlations other traits. Genetic variability indicated flowering exhibited high heritability (0.7) advance (19.6%) percent mean, suggesting strong control, while displayed extremely low h2 (0.003). total 351,692 markers 10 chromosomes we have been refining filtered SNPs. Manhattan plots particularly height. Significant time, yield. 176 varying scores effects. Multiple linked these highlight complexity interactions studied 36 unique 12 QTNs. Notable concentrated on 1, 2, 3, reinforcing importance regions efforts. Candidate gene regulating stress response, could serve targets enhancement. In our study, successfully identified, are resilience. Genes Sobic.001G196700 Sobic.002G183400 critical regulators floral development. stress-responsive Sobic.005G176100 (a mannose-6-phosphate isomerase), emphasizes resilience cultivation under adverse conditions. Additionally, Sobic.003G324400 Sobic.004G178300 height weight, making them valuable enhancement programs. enhances understanding crucial identifies important offering insights marker-assisted breeding. time emphasizing need targeted efforts maximize productivity.

Язык: Английский

Multi-locus genome-wide association mapping for major agronomic and yield-related traits in sorghum (Sorghum bicolor (L.) moench) landraces DOI Creative Commons

Addisu Getahun,

Admas Alemu,

Habte Nida

и другие.

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Март 28, 2025

Sorghum is a vital cereal crop for over 750 million people, ranking 5th globally. It has multiple purposes, including food, feed, and biofuels, essential in Ethiopia, which rich genetic diversity of various agroecological zones. Explore marker-trait associations (MTAs) to identify quantitative trait nucleotides (QTNs) new candidate genes associated with agronomic yield contributing traits Ethiopian sorghum landraces using multi-locus GWAS models assist the genomic-assisted breeding strategies. This study investigates basis through Genome-Wide Association Studies (ML-GWAS). 216 landraces, improved varieties, check cultivars were obtained from Biodiversity Institute National Improvement Program this study. The experiment was conducted two cropping seasons, employing an α-lattice design phenotyping key such as days flowering, maturity, plant height, seed number per plant, grain yield, thousand weight. A mixed linear model (MLM) used analyze phenotypic data estimate parameters variances broad sense heritability. GBS ApeKI restriction enzyme provided 50,165 high-quality SNP markers. six ML-GWAS identified significant QTNs LOD score threshold value ≥ 4.0. analysis revealed major across chromosomes, supported by stringent filtering criterion that ensured reliability. Co-localization known QTLs explored QTL Atlas database within QTN regions, providing architecture influencing performance via Phytozome platform biomaRt package. Pearson correlation among most traits, p-values less than 0.0001, except showed lower correlations other traits. Genetic variability indicated flowering exhibited high heritability (0.7) advance (19.6%) percent mean, suggesting strong control, while displayed extremely low h2 (0.003). total 351,692 markers 10 chromosomes we have been refining filtered SNPs. Manhattan plots particularly height. Significant time, yield. 176 varying scores effects. Multiple linked these highlight complexity interactions studied 36 unique 12 QTNs. Notable concentrated on 1, 2, 3, reinforcing importance regions efforts. Candidate gene regulating stress response, could serve targets enhancement. In our study, successfully identified, are resilience. Genes Sobic.001G196700 Sobic.002G183400 critical regulators floral development. stress-responsive Sobic.005G176100 (a mannose-6-phosphate isomerase), emphasizes resilience cultivation under adverse conditions. Additionally, Sobic.003G324400 Sobic.004G178300 height weight, making them valuable enhancement programs. enhances understanding crucial identifies important offering insights marker-assisted breeding. time emphasizing need targeted efforts maximize productivity.

Язык: Английский

Процитировано

0