Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 10, 2023
Abstract
The
cell-derived
vesicles
(CDVs)
obtained
using
a
proprietary
extrusion
process
are
the
foundation
of
BioDrone™
platform
technology.
With
superior
productivity
and
versatility,
this
technology
has
garnered
increasing
attention
in
broad
applications,
particularly
as
drug
delivery
vehicle.
Previously,
we
showed
that
CDVs
exhibited
varying
levels
expression
for
tetraspanin
organelle
membrane
markers
while
revealing
no
discernible
differences
physical
characteristics
compared
to
naturally
produced
extracellular
(EVs).
To
further
understand
utilize
therapeutic
potentials
CDVs,
more
comprehensive
study
protein
profiles
is
necessary.
In
addition,
it
crucial
validate
from
indeed
intact
lipid
rather
than
other
impurities.
Here,
multiple
batches
EVs
HEK293
cells.
were
subjected
same
purification
processes
subsequent
proteome
particle
analyses.
analyses
revealed
unique
signatures
between
EVs,
parental
Extensive
identified
nine
most
prominent
abundant
cells
EVs.
Subsequent
western
blotting
nanoparticle
flow
cytometry
confirmed
CD63,
lysosome-associated
glycoprotein
1
(LAMP1),
nicastrin
(NCSTN)
highly
enriched
whereas
CD81,
CD9,
prostaglandin
F2
receptor
negative
regulator
(PTGFRN)
This
highlights
composition
marker
signature
distinct
Lastly,
demonstrated
90%
genuine
by
combining
two
different
classes
vesicle
labeling
dyes
detergents
disrupt
membranes.
indicates
our
compatible
with
well-characterized
EV
production
methods.
robust
CDV
will
also
facilitate
engineering
achieve
enhanced
effects
or
tissue-selective
cargo
delivery.
ACS Omega,
Год журнала:
2024,
Номер
9(47), С. 46664 - 46678
Опубликована: Ноя. 13, 2024
In
recent
decades,
the
development
and
application
of
nonviral
vectors,
such
as
liposomes
lipidic
nanoparticles,
for
gene
therapy
drug
delivery
have
seen
substantial
progress.
The
interest
in
physicochemical
properties
structures
complexes
liposome/DNA
liposome/RNA
is
due
to
their
potential
substitute
viruses
carriers
drugs
or
genetic
material
into
cells
with
minimal
cytotoxicity,
which
could
lead
use
therapy.
Initially,
cationic
were
utilized
DNA
vectors;
subsequently,
different
molecules,
polymers,
incorporated
enhance
transfection
efficiency.
Additionally,
liposome/protein
been
developed
vectors
treatment
diseases.
most
relevant
internalization
pathways
these
few
results
obtained
using
targeted
nontargeted
are
discussed
below.
high
cytotoxicity
represents
a
significant
challenge
delivery.
Anionic
offer
promising
alternative
address
limitations
conventional
liposomes,
including
immune
response,
short
circulation
time,
low
toxicity.
This
review
will
discuss
advantages
novel
anionic
liposome-based
systems
that
emerged
result.
advent
designs
manufacturing
techniques
has
facilitated
innovative
systems,
designated
lipid
nanoparticles
(LNPs),
serve
highly
efficacious
regulators
system.
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 31, 2024
Abstract
Photodynamic
therapy
(PDT)
is
a
promising
cancer
treatment,
but
its
clinical
use
limited
by
nontargeted
photosensitizers
(PS)
that
accumulate
in
normal
tissues,
causing
adverse
effects,
and
poor
penetration
tumor
tissues
due
to
the
dense
extracellular
matrix
(ECM).
Here
an
innovative
approach
presented
using
cell‐derived
nanovesicles
(CNVs)
engineered
with
chimeric
antigen
receptor
(CAR)
hyaluronidase
PH20
enhance
targeted
PDT.
The
CAR–PH20
CNVs,
loaded
photosensitizer
pheophorbide
(PheoA),
specifically
target
HER2‐expressing
cells
degrade
hyaluronic
acid
microenvironment
(TME),
improving
drug
distribution.
In
vitro
vivo
experiments
demonstrate
increased
reactive
oxygen
species
(ROS)
generation,
improved
retention,
enhanced
therapeutic
efficacy
compared
conventional
methods.
When
combined
laser
irradiation,
these
CNVs
induce
significant
cell
apoptosis
inhibit
growth
mouse
models,
while
minimizing
toxicity
tissues.
This
platform
offers
strategy
for
targeted,
TME‐modulating
PDT
efficacy,
reduced
side
marking
advance
nanodrug‐based
therapies.
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 10, 2023
Abstract
The
cell-derived
vesicles
(CDVs)
obtained
using
a
proprietary
extrusion
process
are
the
foundation
of
BioDrone™
platform
technology.
With
superior
productivity
and
versatility,
this
technology
has
garnered
increasing
attention
in
broad
applications,
particularly
as
drug
delivery
vehicle.
Previously,
we
showed
that
CDVs
exhibited
varying
levels
expression
for
tetraspanin
organelle
membrane
markers
while
revealing
no
discernible
differences
physical
characteristics
compared
to
naturally
produced
extracellular
(EVs).
To
further
understand
utilize
therapeutic
potentials
CDVs,
more
comprehensive
study
protein
profiles
is
necessary.
In
addition,
it
crucial
validate
from
indeed
intact
lipid
rather
than
other
impurities.
Here,
multiple
batches
EVs
HEK293
cells.
were
subjected
same
purification
processes
subsequent
proteome
particle
analyses.
analyses
revealed
unique
signatures
between
EVs,
parental
Extensive
identified
nine
most
prominent
abundant
cells
EVs.
Subsequent
western
blotting
nanoparticle
flow
cytometry
confirmed
CD63,
lysosome-associated
glycoprotein
1
(LAMP1),
nicastrin
(NCSTN)
highly
enriched
whereas
CD81,
CD9,
prostaglandin
F2
receptor
negative
regulator
(PTGFRN)
This
highlights
composition
marker
signature
distinct
Lastly,
demonstrated
90%
genuine
by
combining
two
different
classes
vesicle
labeling
dyes
detergents
disrupt
membranes.
indicates
our
compatible
with
well-characterized
EV
production
methods.
robust
CDV
will
also
facilitate
engineering
achieve
enhanced
effects
or
tissue-selective
cargo
delivery.
