Preliminary Data on Silybum marianum Metabolites: Comprehensive Characterization, Antioxidant, Antidiabetic, Antimicrobial Activities, LC-MS/MS Profiling, and Predicted ADMET Analysis
Metabolites,
Год журнала:
2025,
Номер
15(1), С. 13 - 13
Опубликована: Янв. 3, 2025
Silybum
marianum
extract,
obtained
via
microwave-enhanced
extraction,
was
evaluated
for
its
antioxidant,
antidiabetic,
and
antimicrobial
activities
to
explore
therapeutic
potential.
The
extraction
performed
using
techniques,
LC-MS/MS
employed
profile
the
metabolites
in
extract.
Total
phenolic
flavonoid
contents
were
quantified
spectrophotometric
methods.
Antioxidant
activity
assessed
DPPH,
ABTS,
CUPRAC,
Phenanthroline,
FRAP
assays.
Enzyme
inhibition
assays
conducted
evaluate
antidiabetic
against
α-glucosidase
α-amylase.
Antimicrobial
determined
disc
diffusion
method,
silico
ADMET
drug-likeness
analyses
key
metabolites.
extract
contained
251.2
±
1.2
mg
GAE/g
of
total
phenolics
125.1
1.6
QE/g
flavonoids,
with
33
identified,
including
acids,
tannins,
flavolignans.
Strong
antioxidant
observed,
IC50
values
19.2
2.3
μg/mL
(DPPH),
7.2
1.7
(ABTS),
22.2
(CUPRAC),
35.2
1.8
(Phenanthroline),
24.1
(FRAP).
Antidiabetic
effects
significant,
18.1
(α-glucosidase)
26.5
1.3
(α-amylase).
demonstrated
zones
8.9
1.1
mm
(Bacillus
subtilis),
12.6
(Escherichia
coli),
8.2
(Fusarium
oxysporum),
9.2
(Aspergillus
niger).
In
showed
high
absorption,
favorable
metabolism
excretion,
minimal
toxicity,
no
hERG
channel
or
hepatotoxicity.
comprehensive
results
highlight
significant
S.
suggesting
potential
preventive
applications.
Язык: Английский
Simultaneous Quantification of Phenolic Compounds in the Leaves and Roots of Peucedanum japonicum Thunb. Using HPLC-PDA with Various Extraction Solvents
Horticulturae,
Год журнала:
2025,
Номер
11(3), С. 334 - 334
Опубликована: Март 19, 2025
This
study
investigated
the
extraction
and
quantification
of
specific
phenolic
compounds,
including
chlorogenic
acid
several
pyranocoumarin
derivatives,
from
leaves
roots
Peucedanum
japonicum.
Using
high-performance
liquid
chromatography,
this
aimed
to
optimize
methodologies
with
different
solvents
maximize
yield
bioactive
compounds.
The
process
involved
meticulous
steps,
reflux
solvent
evaporation,
total
content
was
assessed
using
a
spectrophotometric
assay.
results
demonstrated
that
ethanol
methanol
were
effective
in
extracting
acid,
yielding
up
47.71
mg/g
tannic
equivalent
MeOH
extracts
roots.
Conversely,
acetone
superior
for
extraction,
achieving
coumarin
exceeding
100
root
extracts.
Notably,
pyranocoumarins
found
be
more
concentrated
compared
leaves,
supporting
hypothesis
are
reservoir
these
emphasized
critical
roles
selections
profiling
compounds
P.
japonicum
provided
valuable
insights
future
research
into
its
pharmacological
potential.
findings
may
serve
as
foundation
further
studies,
enhancing
understanding
medicinal
properties
context
traditional
East
Asian
medicine.
Язык: Английский
Optimization of Ultrasonic-Assisted Extraction of Diene Urushiol from Lacquer Tree Leaves Using Response Surface Methodology
F. Xia,
Haojiang He,
Juan Ma
и другие.
Molecules,
Год журнала:
2025,
Номер
30(8), С. 1663 - 1663
Опубликована: Апрель 8, 2025
Lacquer
trees
are
an
important
economic
tree
species
in
China,
and
raw
lacquer
is
its
main
secondary
metabolite.
Polyphenolic
compounds
the
primary
components
of
lacquer,
among
which
diene
urushiol
exhibits
high
inhibitory
activity
against
reverse
transcriptase
acquired
immunodeficiency
syndrome
(AIDS).
Therefore,
this
study
established
optimized
ultrasound-assisted
extraction
process
from
leaves.
Based
on
single-factor
experiments
number
extractions,
time,
temperature,
solvent
to
solid
ratio,
Box-Behnken
Design
response
surface
methodology
was
employed
obtain
optimal
process,
included
three
time
55
min,
temperature
50
°C,
a
ratio
10:1
mL/g.
Under
these
conditions,
content
4.56
mg/g
(FW),
bore
no
significant
difference
theoretical
value
4.69
indicating
good
model
fit.
(RSM)
can
be
used
optimize
This
method
lays
foundation
for
comprehensive
development
utilization
resources.
Язык: Английский
Extraction and Characterization of Biological Phytoconstituents of Commiphora gileadensis Leaves Using Soxhlet Method
Processes,
Год журнала:
2024,
Номер
12(8), С. 1567 - 1567
Опубликована: Июль 26, 2024
Commiphora
gileadensis
is
a
medicinal
plant
with
wide
range
of
biological
characteristics.
Many
medical
diseases
can
be
treated
using
the
leaves
C.
gileadensis,
including
bacterial
infections,
inflammatory
illnesses,
and
wounds.
As
result,
Soxhlet
extraction
method
was
used
to
extract
phenolic
components
measure
recovery
yields
from
leaf.
The
impacts
parameters
(extraction
time
30–150
min,
sample/solvent
ratio
1:20–1:40
g/mL,
concentration
ethanol
solvent
20–100%
v/v)
on
total
flavonoid
content
(TFC),
(TPC),
yield
were
investigated
one-factor-at-a-time
(OFAT)
technique.
Fourier
transform
infrared
spectroscopy
(FTIR)
gas
chromatography–mass
(GC–MS)
analyses
have
been
employed
evaluate
extracts
for
presence
various
phytochemicals.
According
results,
leafs
ethanolic
extracted
via
process
achieved
maximum
at
90
min
time,
feed/solvent
1:30
40%
v/v
concentration.
These
were:
=
23.20
±
0.10%
w/w,
TPC
59.93
1.33
mg
GAE/g
d.w.,
TFC
19.65
1.77
QE/g
d.w..
Further,
20
excellent
antioxidant
characteristics
found
in
leaf
method.
Язык: Английский
Microwave-Assisted Extraction of Phenolic Compounds from Commiphora gileadensis Leaf and their Characterization
Results in Engineering,
Год журнала:
2024,
Номер
unknown, С. 102892 - 102892
Опубликована: Сен. 1, 2024
Язык: Английский
Greenness assessment approach for spectrofluorimetric detection of daptomycin using metal oxide nanosensors synthesized via Commiphora myrrha extract in the presence of organizing solution
Results in Chemistry,
Год журнала:
2024,
Номер
unknown, С. 101948 - 101948
Опубликована: Дек. 1, 2024
Язык: Английский