Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Апрель 26, 2024
The
endemic
outbreak
of
SADS-CoV
has
resulted
in
economic
losses
and
potentially
threatened
the
safety
China's
pig
industry.
molecular
epidemiology
herds
been
investigated
many
provinces
China.
However,
there
are
no
data
over
a
long-time
span,
is
lack
extensive
serological
surveys
to
assess
prevalence
Chinese
swine
since
discovery
SADS-CoV.
In
this
study,
an
indirect
anti-SADS-CoV
IgG
enzyme-linked
immunosorbent
assay
(ELISA)
based
on
S1
protein
was
established
investigate
seroprevalence
herds.
Cross-reactivity
assays,
immunofluorescence,
western
blotting
assays
showed
that
developed
ELISA
had
excellent
specificity.
total,
12,978
serum
samples
from
29
provinces/municipalities/autonomous
regions
China
were
tested
2022
2023.
results
general
59.97%,
with
ranging
16.7%
77.12%
different
42.61%
68.45%
months.
widely
prevalent
China,
its
higher
Northeast
North
Central
than
other
regions.
Among
four
seasons,
highest
spring
lowest
autumn.
study
provide
profile
facilitating
understanding
pigs.
More
importantly,
beneficial
formulating
preventive
control
measures
for
may
directions
vaccine
development.
Frontiers in Immunology,
Год журнала:
2023,
Номер
14
Опубликована: Май 22, 2023
Swine
acute
diarrhoea
syndrome
coronavirus
(SADS-CoV),
which
is
a
recently
discovered
enteric
coronavirus,
the
major
aetiological
agent
that
causes
severe
clinical
and
intestinal
pathological
damage
in
pigs,
it
has
caused
significant
economic
losses
to
swine
industry.
Nonstructural
protein
5,
also
called
3C-like
protease,
cleaves
viral
polypeptides
host
immune-related
molecules
facilitate
replication
immune
evasion.
Here,
we
demonstrated
SADS-CoV
nsp5
significantly
inhibits
Sendai
virus
(SEV)-induced
production
of
IFN-β
inflammatory
cytokines.
targets
mRNA-decapping
enzyme
1a
(DCP1A)
via
its
protease
activity
inhibit
IRF3
NF-κB
signaling
pathways
order
decrease
cytokine
production.
We
found
histidine
41
cystine
144
residues
are
critical
for
cleavage
activity.
Additionally,
form
DCP1A
with
mutation
glutamine
343
residue
resistant
nsp5-mediated
stronger
ability
infection
than
wild-type
DCP1A.
In
conclusion,
our
findings
reveal
an
important
interferon
antagonist
enhance
understanding
evasion
by
alpha
coronaviruses.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(18), С. 10640 - 10654
Опубликована: Апрель 25, 2024
Coronaviruses
have
consistently
posed
a
major
global
concern
in
the
field
of
livestock
industry
and
public
health.
However,
there
is
currently
lack
efficient
drugs
with
broad-spectrum
antiviral
activity
to
address
challenges
presented
by
emerging
mutated
strains
or
drug
resistance.
Additionally,
method
for
identifying
multitarget
also
insufficient.
Aminopeptidase
N
(APN)
3C-like
proteinase
(3CLpro)
represent
promising
targets
host-directed
virus-directed
strategies,
respectively,
development
effective
against
various
coronaviruses.
In
this
study,
maduramycin
ammonium
demonstrated
effect
targeting
both
proteins.
The
binding
domains
4
Å
from
ligand
target
proteins
shared
structural
similarity,
suggesting
that
screening
designing
based
on
these
might
exhibit
highly
activity.
Furthermore,
it
was
identified
polyether
ionophores'
ability
carry
zinc
ion
be
one
reasons
why
they
were
able
APN
effect.
findings
experiment
provide
novel
perspectives
future
design,
while
offering
valuable
references
utilization
ionophores
management
Frontiers in Veterinary Science,
Год журнала:
2023,
Номер
9
Опубликована: Март 2, 2023
The
regional
outbreak
of
the
Swine
acute
diarrhea
syndrome
coronavirus
(SADS-CoV)
has
seriously
threatened
swine
industry.
There
is
an
urgent
need
to
discover
safe
and
effective
vaccines
contain
them
quickly.
spike
protein
mediates
virus
entry
into
host
cells,
one
most
important
antigenic
determinants
a
potential
vaccine
target.
Therefore,
this
study
aims
conduct
predictive
analysis
epitope
S
B
cells
T
(MHC
class
I
II)
by
immunoinformatics
methods
screening
identifying
protective
epitopes
that
induce
major
neutralized
antibodies
activate
immune
responses
construct
vaccines.
explored
primary,
secondary,
tertiary
structures,
disulfide
bonds,
docking,
response
simulation,
seamless
cloning
results
show
dominant
high
conservativeness
coverage
IFN-γ,
IL-4-positive
Th
epitope,
CTL
epitope.
constructed
interacts
stably
with
TLR-3
receptors,
simulation
shows
good
immunogenicity,
which
could
effectively
humoral
cellular
immunity.
After
codon
optimization,
it
was
highly
likely
be
efficiently
expressed
in
Escherichia
coli
K12
expression
system.
will
provide
new
theoretical
basis
for
design
SADS-CoV
antiviral
drugs
related
research
on
coronaviruses
such
as
SARS-CoV-2.
Emerging Microbes & Infections,
Год журнала:
2023,
Номер
12(2)
Опубликована: Авг. 16, 2023
Zoonotic
transmission
of
coronaviruses
(CoVs)
poses
a
serious
public
health
threat.
Swine
acute
diarrhea
syndrome
coronavirus
(SADS-CoV),
originating
from
bat
HKU2-related
CoV,
causes
devastating
swine
diseases
and
high
risk
spillover
to
humans.
Currently,
licensed
therapeutics
that
can
prevent
potential
human
outbreaks
are
unavailable.
Identifying
the
cellular
proteins
restrict
viral
infection
is
imperative
for
developing
effective
interventions
therapeutics.
We
utilized
large-scale
cDNA
screening
identified
transmembrane
protein
53
(TMEM53)
as
novel
cell-intrinsic
SADS-CoV
restriction
factor.
The
inhibitory
effect
TMEM53
on
was
found
be
independent
canonical
type
I
interferon
responses.
Instead,
interacts
with
non-structural
12
(NSP12)
disrupts
RNA-dependent
RNA
polymerase
(RdRp)
complex
assembly
by
interrupting
NSP8-NSP12
interaction,
thus
suppressing
RdRp
activity
synthesis.
Deleting
domain
resulted
in
abrogation
TMEM53-NSP12
interaction
antiviral
activity.
Importantly,
exhibited
broad
against
multiple
CoVs.
Our
findings
reveal
role
pave
way
host-directed
CoV
infection.
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2024,
Номер
14
Опубликована: Апрель 26, 2024
The
endemic
outbreak
of
SADS-CoV
has
resulted
in
economic
losses
and
potentially
threatened
the
safety
China's
pig
industry.
molecular
epidemiology
herds
been
investigated
many
provinces
China.
However,
there
are
no
data
over
a
long-time
span,
is
lack
extensive
serological
surveys
to
assess
prevalence
Chinese
swine
since
discovery
SADS-CoV.
In
this
study,
an
indirect
anti-SADS-CoV
IgG
enzyme-linked
immunosorbent
assay
(ELISA)
based
on
S1
protein
was
established
investigate
seroprevalence
herds.
Cross-reactivity
assays,
immunofluorescence,
western
blotting
assays
showed
that
developed
ELISA
had
excellent
specificity.
total,
12,978
serum
samples
from
29
provinces/municipalities/autonomous
regions
China
were
tested
2022
2023.
results
general
59.97%,
with
ranging
16.7%
77.12%
different
42.61%
68.45%
months.
widely
prevalent
China,
its
higher
Northeast
North
Central
than
other
regions.
Among
four
seasons,
highest
spring
lowest
autumn.
study
provide
profile
facilitating
understanding
pigs.
More
importantly,
beneficial
formulating
preventive
control
measures
for
may
directions
vaccine
development.
