Frontiers in Chemical Biology,
Год журнала:
2024,
Номер
3
Опубликована: Дек. 20, 2024
Inflammation-related
disorders,
such
as
autoimmune
diseases
and
cancer,
impose
a
significant
global
health
burden.
Zinc
finger
proteins
(ZFs)
are
ubiquitous
metalloproteins
which
regulate
inflammation
many
biological
signaling
pathways
related
to
growth,
development,
immune
function.
Numerous
ZFs
involved
in
the
nuclear
factor
kappa-light-chain-enhancer
of
activated
B
cells
(NFκB)
pathway,
associating
them
with
inflammation-related
that
feature
chronically
elevated
pro-inflammatory
cytokines.
This
review
highlights
predominance
NFκB-related
summarizes
breadth
functions
these
perform.
The
cysteine-specific
post-translational
modification
(PTM)
persulfidation
is
also
discussed
context
cysteine-rich
ZFs,
including
what
known
from
few
available
reports
on
functional
implications
ZF
persulfidation.
Persulfidation,
mediated
by
endogenously
produced
hydrogen
sulfide
(H
2
S),
has
recently
established
role
inflammation.
work
will
summarize
connections
between
potential
inform
development
therapies.
Inorganics,
Год журнала:
2024,
Номер
12(12), С. 316 - 316
Опубликована: Дек. 6, 2024
Iron–sulfur
(Fe-S)
clusters
are
essential
cofactors
found
in
many
proteins
the
mitochondria,
cytosol,
and
nucleus
of
cell.
These
versatile
may
undergo
reversible
oxidation–reduction
reactions
to
enable
electron
transfers;
they
be
structural
confer
stability
a
folded
protein;
regulatory
transduce
an
iron
signal
that
alters
function
or
recipient
protein.
Of
nearly
70
described
mammalian
cells
bind
Fe-S
clusters,
about
half
localize
exclusively
partially
nucleus,
where
required
for
DNA
replication
repair,
telomere
maintenance,
transcription,
mitosis,
cell
cycle
control.
Most
nuclear
cluster
interact
with
DNA,
including
polymerases,
primase,
helicases,
glycosylases.
However,
specific
roles
enzymatic
activities
these
their
interplay
remain
matter
debate.
Defects
metallation
cause
genome
instability
alter
regulation
division
proliferation,
which
hallmarks
various
genetic
diseases
cancers.
Here,
we
provide
inventory
cluster-binding
discuss
types,
binding
sites,
process
acquisition,
potential
proteins.
questions
unresolved.
We
highlight
critical
gaps
our
understanding
delivery
client
proteins,
mechanistic
play
enzymes.
Taken
together,
this
review
brings
focus
human
as
hotspot
aims
inspire
new
research
on
metabolism
maintenance
integrity.
Chemical Reviews,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 10, 2024
Transition
metals
function
as
structural
and
catalytic
cofactors
for
a
large
diversity
of
proteins
enzymes
that
collectively
comprise
the
metalloproteome.
Metallostasis
considers
all
cellular
processes,
notably
metal
sensing,
metalloproteome
remodeling,
trafficking
(or
allocation)
ensure
functional
integrity
adaptability
Bacteria
employ
both
protein
RNA-based
mechanisms
sense
intracellular
transition
bioavailability
orchestrate
systems-level
outputs
maintain
metallostasis.
In
this
review,
we
contextualize
metallostasis
by
briefly
discussing
specialized
roles
play
in
biology.
We
then
offer
comprehensive
perspective
on
metalloregulatory
metal-sensing
riboswitches,
defining
general
principles
within
each
sensor
superfamily
capture
how
specificity
is
encoded
sequence,
selectivity
can
be
leveraged
downstream
synthetic
biology
biotechnology
applications.
This
followed
discussion
recent
work
highlights
selected
outputs,
including
remodeling
allocation
metallochaperones
to
client
compartments.
close
places
where
more
needed
fill
gaps
our
understanding
Biochemistry,
Год журнала:
2024,
Номер
64(1), С. 156 - 169
Опубликована: Дек. 16, 2024
Ran-binding
domain-containing
protein
2
(ZRANB2)
is
a
zinc
finger
(ZF)
that
plays
key
role
in
alternative
splicing.
ZRANB2
composed
of
two
ZF
domains
contain
four
invariant
cysteine
residues
per
domain.
binds
RNA
targets
AGGUAA
sequence
motifs.
Three
constructs
ZRANB2,
ZRANB2-ZF1
(first
domain),
ZRANB2-ZF2
(second
and
ZRANB2-2D
(both
domains),
were
isolated
the
apo
form
shown
to
bind
Zn(II)
via
UV-visible-monitored
competitive
titrations
with
Co(II)
as
spectroscopic
probe.
Zn
binding
each
construct
led
adoption
limited
secondary
structure
domain,
measured
by
circular
dichroism
(CD).
Hydrogen-deuterium
exchange
coupled
mass
spectrometry
(HDX-MS)
two-domain
construct,
ZRANB2-2D,
revealed
both
adopt
more
rigid
upon
binding.
first
domain
resulted
greater
decrease
conformational
dynamics
than
second
TRA2B
pre-mRNA,
physiological
splicing
target,
was
fluorescence
anisotropy
(FA),
high-affinity
found
require
coordination
domains.
HDX-MS
well
optimized
sequences
single
double
hexamer
additional
protection
from
H/D
for
presence
RNA.
Here,
observed
suggesting
larger
effect
on
dynamics.
A
model
zinc-mediated
proposed.
Frontiers in Chemical Biology,
Год журнала:
2024,
Номер
3
Опубликована: Дек. 20, 2024
Inflammation-related
disorders,
such
as
autoimmune
diseases
and
cancer,
impose
a
significant
global
health
burden.
Zinc
finger
proteins
(ZFs)
are
ubiquitous
metalloproteins
which
regulate
inflammation
many
biological
signaling
pathways
related
to
growth,
development,
immune
function.
Numerous
ZFs
involved
in
the
nuclear
factor
kappa-light-chain-enhancer
of
activated
B
cells
(NFκB)
pathway,
associating
them
with
inflammation-related
that
feature
chronically
elevated
pro-inflammatory
cytokines.
This
review
highlights
predominance
NFκB-related
summarizes
breadth
functions
these
perform.
The
cysteine-specific
post-translational
modification
(PTM)
persulfidation
is
also
discussed
context
cysteine-rich
ZFs,
including
what
known
from
few
available
reports
on
functional
implications
ZF
persulfidation.
Persulfidation,
mediated
by
endogenously
produced
hydrogen
sulfide
(H
2
S),
has
recently
established
role
inflammation.
work
will
summarize
connections
between
potential
inform
development
therapies.
