bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 19, 2023
Abstract
The
Coronavirus
Disease
2019
(COVID-19)
pandemic
caused
by
Severe
Acute
Respiratory
Syndrome
2
(SARS-CoV-2)
has
taken
its
toll
on
worldwide
public
health
infrastructure.
SARS-CoV-2
is
reported
to
exhibit
wide
tissue
tropism,
contributing
severe
pathogenicity
that
often
culminates
in
multiple-organ
failure.
onslaught
of
this
disease
intensified
due
the
emergence
variants
concern
(VOC),
such
as
Delta
and
Omicron.
These
have
been
linked
gastrointestinal
(GI)
symptoms,
suggesting
a
potential
fecal-oral
route
viral
transmission.
Here
we
compared
broad
tropism
ancestral
Hong-Kong
(SARS-CoV-2
HK)
against
Omicron
VOCs
aa
hamster
model
analyzing
samples
collected
from
upper
lower
respiratory
system
GI
tract.
We
observed
an
overall
increase
vRNA
load
pro-
inflammatory
cytokines,
especially
tracts
animals
infected
with
virus,
indicating
selective
virus
pathology
these
tissues.
However,
no
apparent
spike
was
large
intestine
fecal
matter.
Overall,
our
research
investigates
range
tissues
various
strains
can
infect
hamsters
presents
evidence
supporting
increased
preference
for
infecting
During
the
three
years
following
emergence
of
COVID-19
pandemic,
African
continent,
like
other
regions
world,
was
substantially
impacted
by
COVID-19.
In
Morocco,
pandemic
has
been
marked
and
spread
several
SARS-CoV-2
variants,
leading
to
a
substantial
increase
in
incidence
infections
deaths.
Nevertheless,
there
persistent
lack
comprehensive
understanding
regarding
genetic
diversity,
evolution,
epidemiology
viral
lineages.
This
study
sought
deepen
genomic
through
retrospective
analysis.The
main
objective
this
analyze
diversity
identify
distinct
lineages,
as
well
assess
their
evolution
during
using
approaches.Furthermore,
key
mutations
functional
proteins
across
different
lineages
were
highlighted
along
with
an
analysis
relationships
amongst
these
strains
better
understand
evolutionary
pathways.
A
total
2274
sequences
SARS-CoV-2,
acquired
from
Morocco
period
2020
2023,
extracted
GISAID
EpiCoV
database
subjected
analysis.
Lineages
clades
classified
according
nomenclature
GISAID,
Nextstrain,
Pangolin.
The
conducted
reported
accordance
STROBE
(Strengthening
Reporting
Observational
Studies
Epidemiology)
guidelines.
An
exhaustive
led
identification
157
PANGO
including
notable
such
B.1,
B.1.1,
B.1.528
B.1.177,
variants
B.1.1.7,
B.1.621,
B.1.525,
B.1.351,
B.1.617.1,
B.1.617.2
its
sub-lineages
AY.33,
AY.72,
AY.112,
AY.121
that
evolved
over
time
before
being
supplanted
Omicron
December
2021.
Among
analyzed,
sub-variants
had
prevalence
59.5%.
most
predominant
21K,
21L,
22B,
which
are
respectively
related
phylogenetically
BA.1,
BA.2,
BA.5.
June
2022,
rapidly
observed
recrudescence
cases
infection,
concurrent
coexistence
subvariants
clade
22B
BA.5.2.20,
BA.5,
BA.5.1,
BA.5.2.1,
BF.5,
supplanting
BA.1
(Clade
display
21K)
BA.2
21L),
became
marginal.
However,
XBB
(clade
22F)
progeny
XBB.1.5(23A),
XBB.1.16(23B),
CH.1.1(23C),
XBB.1.9(23D),
XBB.2.3(23E),
EG.5.1(23F),
XBB.1.5.70
(23G)
have
sporadically.
Furthermore,
mutations,
H69del/V70del,
G142D,
K417N,
T478K,
E484K,
E484A,
L452R,
F486P,
N501Y,
Q613H,
D614G,
P681H/R,
known
for
implications
transmissibility
infectivity
ability
escape
antibodies,
identified.
identified
various
involved
Moroccan
isolates,
analyzed
trends.
genetically
closely
linked
original
highlighting
importance
maintaining
continuous
surveillance
expansion
vaccine
coverage
would
help
protect
patients
against
more
severe
clinical
disease.
These
findings
also
provide
robust
basis
role
transmission,
immune
evasion,
reinfection.
Journal of Pure and Applied Microbiology,
Год журнала:
2023,
Номер
17(1), С. 385 - 394
Опубликована: Март 1, 2023
SARS-CoV-2
is
continually
evolving
with
the
emergence
of
new
variants
increased
viral
pathogenicity.
The
heavily
mutated
Omicron
(B.1.1.529)
spike
protein
mutations
are
known
to
mediate
its
higher
transmissibility
and
immune
escape
that
has
brought
newer
challenges
for
global
public
health
contain
infection.
One
come
up
a
therapeutic
strategy
against
virus
so
as
effectively
infection
spread.
Natural
phytochemicals
being
considered
significant
source
bioactive
compounds
possessing
an
antiviral
potential.
Being
promising
anticancer
chemo-preventive
agent,
Silybin
holds
potential
be
used
therapeutic.
In
present
study,
molecular
docking
(7QNW)
was
carried
out.
Molecular
results
showed
greater
stability
in
active
site
suitable
binding
mode
interactions.
study
reveals
block
host
ACE2
receptor-viral
binding;
thereby
inhibiting
entry
human
cells.
Therefore,
may
further
developed
medication
ability
combat
Omicron.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 19, 2023
Abstract
The
Coronavirus
Disease
2019
(COVID-19)
pandemic
caused
by
Severe
Acute
Respiratory
Syndrome
2
(SARS-CoV-2)
has
taken
its
toll
on
worldwide
public
health
infrastructure.
SARS-CoV-2
is
reported
to
exhibit
wide
tissue
tropism,
contributing
severe
pathogenicity
that
often
culminates
in
multiple-organ
failure.
onslaught
of
this
disease
intensified
due
the
emergence
variants
concern
(VOC),
such
as
Delta
and
Omicron.
These
have
been
linked
gastrointestinal
(GI)
symptoms,
suggesting
a
potential
fecal-oral
route
viral
transmission.
Here
we
compared
broad
tropism
ancestral
Hong-Kong
(SARS-CoV-2
HK)
against
Omicron
VOCs
aa
hamster
model
analyzing
samples
collected
from
upper
lower
respiratory
system
GI
tract.
We
observed
an
overall
increase
vRNA
load
pro-
inflammatory
cytokines,
especially
tracts
animals
infected
with
virus,
indicating
selective
virus
pathology
these
tissues.
However,
no
apparent
spike
was
large
intestine
fecal
matter.
Overall,
our
research
investigates
range
tissues
various
strains
can
infect
hamsters
presents
evidence
supporting
increased
preference
for
infecting
