GPD1L‐Mediated Glycerophospholipid Metabolism Dysfunction in Women With Diminished Ovarian Reserve: Insights From Pseudotargeted Metabolomic Analysis of Follicular Fluid
Cell Proliferation,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 20, 2025
ABSTRACT
Diminished
ovarian
reserve
(DOR)
is
a
pathological
condition
characterised
by
reduced
function,
which
refers
to
the
decreased
quality
and
quantity
of
oocytes,
potentially
causing
female
infertility
various
health
issues.
Follicular
fluid
(FF)
serves
as
microenvironment
for
follicular
development
oocyte
maturation,
gaining
an
in‐depth
understanding
metabolic
state
FF
will
help
us
uncover
key
biological
processes
involved
in
aging,
while
specific
underlying
pathogenic
mechanisms
are
not
fully
understood.
In
this
study,
we
utilised
pseudotargeted
metabolomic
analysis
reveal
glycerophospholipid
metabolism
dysfunction
mediated
GPD1L
DOR
patients.
We
also
found
that
was
downregulated
granulosa
cells
(GCs)
patients,
resulting
increased
cell
apoptosis
mitochondrial
dysfunction.
Moreover,
our
results
demonstrated
expression
could
induce
atresia
impair
mouse
ovaries.
Altogether,
research
suggested
GCs
metabolites
pathway
act
novel
biomarkers
diagnosis,
paving
way
new
theoretical
basis
pathogenesis
DOR.
Язык: Английский
The Warburg hypothesis and the emergence of the mitochondrial metabolic theory of cancer
Journal of Bioenergetics and Biomembranes,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 8, 2025
Abstract
Otto
Warburg
originally
proposed
that
cancer
arose
from
a
two-step
process.
The
first
step
involved
chronic
insufficiency
of
mitochondrial
oxidative
phosphorylation
(OxPhos),
while
the
second
protracted
compensatory
energy
synthesis
through
lactic
acid
fermentation.
His
extensive
findings
showed
oxygen
consumption
was
lower
lactate
production
higher
in
cancerous
tissues
than
non-cancerous
tissues.
considered
both
and
extracellular
as
accurate
markers
for
ATP
OxPhos
glycolysis,
respectively.
Warburg’s
hypothesis
challenged
showing
remained
high
some
cells
despite
elevated
suggesting
largely
unimpaired.
New
information
indicates
neither
nor
are
surrogates
quantification
cells.
also
did
not
know
significant
amount
could
come
glutamine-driven
substrate
level
glutaminolysis
pathway
with
succinate
produced
end
product,
thus
confounding
linkage
to
origin
within
mitochondria.
Moreover,
new
shows
cytoplasmic
lipid
droplets
aerobic
fermentation
biomarkers
insufficiency.
original
can
now
be
linked
more
complete
understanding
how
underlies
dysregulated
cell
growth.
These
address
several
questionable
assumptions
regarding
allowing
field
advance
effective
therapeutic
strategies
less
toxic
metabolic
management
prevention
cancer.
Язык: Английский
Origin and evolution of mitochondrial inner membrane composition
Journal of Cell Science,
Год журнала:
2025,
Номер
138(9)
Опубликована: Апрель 23, 2025
ABSTRACT
Unique
membrane
architectures
and
lipid
building
blocks
underlie
the
metabolic
non-metabolic
functions
of
mitochondria.
During
eukaryogenesis,
mitochondria
likely
arose
from
an
alphaproteobacterial
symbiont
Asgard
archaea-related
host
cell.
Subsequently,
evolved
inner
folds
known
as
cristae
alongside
a
specialized
composition
supported
by
transport
machinery.
Advancements
in
phylogenetic
methods
genomic
metagenomic
data
have
suggested
potential
origins
for
cristae-shaping
protein
complexes,
such
mitochondrial
contact
site
cristae-organizing
system
(MICOS).
MICOS
homologs
function
formation
cristae-like
intracytoplasmic
membranes
(ICMs)
diverse
extant
alphaproteobacteria.
The
machinery
responsible
synthesizing
key
phospholipids
–
which
cooperate
with
proteins
to
establish
architecture
could
also
bacterial
ancestor,
but
its
been
less
explored.
In
this
Review,
we
examine
current
understanding
evolution,
highlighting
distinctions
between
prokaryotic
eukaryotic
mitochondrial-specific
lipids
their
differing
roles
shaping
ICM
architecture,
propose
model
explaining
concurrent
specialization
lipidome
structure
eukaryogenesis.
We
discuss
how
advancements
across
range
disciplines
are
shedding
light
on
multiple
components
co-evolved
support
central
Язык: Английский