A fast-track, high-throughput screening platform for biological nitrification inhibitors discovery based on soil relevant ammonia oxidizing strains DOI Open Access

Alexandros E. Kanellopoulos,

Andrea Malits, Hugo Ribeiro

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 3, 2024

Abstract Nitrogen cycling is critical for ecosystem functioning, with nitrification playing a central role. Excessive nitrification, triggered by heavy nitrogen fertilisation, contributes to reduced use efficiency, nitrate leaching, and nitrous oxide emissions. While synthetic inhibitors (SNIs) help reduce these impacts, their erratic performance environmental risks have shifted focus biological (BNIs) as sustainable alternatives. In vitro bioassays ammonia-oxidising microorganisms (AOM) are valuable BNI discovery research, but often low throughput rely on limited number of mostly non-soil-relevant or genetically modified ammonia-oxidizing bacteria (AOB) strains, lacking validation established BNIs. We present refined fast-track, high-throughput assay screening, utilizing soil-relevant, ecophysiologically phylogenetically diverse AOB ( Nitrosospira multiformis, Nitrosomonas ureae, communis ) archaea (AOA) strains Nitrososphaera viennensis, “ Ca . Nitrosocosmicus franklandianus”), achieving consistent cellular activity density. The was validated SNIs BNIs, showing differences in inhibition efficacy strain sensitivity, literature. As proof concept, root exudates from wheat genotypes were screened, demonstrating distinct profiles. proposed system advances previously available screening systems and, when integrated realistic soil tests, will facilitate the novel BNIs BNI-producing plant genotypes.

Язык: Английский

Planktonic drivers of carbon transformation during different stages of the spring bloom at the Patagonian Shelf-break front, Southwestern Atlantic Ocean DOI Creative Commons

Azul S. Gilabert,

Celeste López‐Abbate, Pedro Flombaum

и другие.

Biogeochemistry, Год журнала: 2025, Номер 168(1)

Опубликована: Янв. 2, 2025

The processes involved in the carbon cycle are essential for marine trophic networks and global climate regulation. Interactions within microbial loop play key roles transformation transport across food web. Argentine Patagonian Shelf Southwestern Atlantic Ocean is a hotspot sequestration. However, our understanding of impacts on cycling this area remains limited. This study examines community structure its role during progression spring bloom along shelf-break adjacent ocean. was studied latitudinal track where we observed gradient Dissolved Organic Matter (DOM) complexity. In northern area, termination characterised by low Chlorophyll-a concentrations, with smaller organisms (Synechococcus) dominating autotrophic plankton biomass, high viral concentrations. DOM showed humification aromaticity, indicating an intensified activity heterotrophic bacteria that followed production phytoplankton-derived DOM. southern mainly attributed to large protist plankton, accompanied abundant bioavailable from recent phytoplankton blooms. These results termination, bacterial refractory compounds significantly immobilises carbon, suggesting potential pathway Additionally, data suggest retention shelf side front efficient transfer community, while influenced Malvinas Current, presents export advection higher degree unutilised origin. findings highlight rapid shifts dynamics driven successions different phases.

Язык: Английский

Процитировано

2

Source characterization of dissolved organic matter in the eastern Beagle Channel from a spring situation DOI

Andrea Malits,

Chiara Monforte,

Clara M. Iachetti

и другие.

Journal of Marine Systems, Год журнала: 2023, Номер 240, С. 103863 - 103863

Опубликована: Фев. 3, 2023

Язык: Английский

Процитировано

13

Terrigenous dissolved organic matter input and nutrient-light-limited conditions on the winter microbial food web of the Beagle Channel DOI

Clara Natalia Rodríguez-Flórez,

Joanna Paczkowska, Jacobo Martín

и другие.

Journal of Marine Systems, Год журнала: 2023, Номер 239, С. 103860 - 103860

Опубликована: Янв. 27, 2023

Язык: Английский

Процитировано

10

Ecological role of pelagic appendicularians in sub-Antarctic coastal and oceanic waters of the Southwest Atlantic Ocean DOI
Nadia M. Alves,

Mariela L. Spinelli,

Jacobo Martín

и другие.

Polar Biology, Год журнала: 2025, Номер 48(1)

Опубликована: Янв. 15, 2025

Язык: Английский

Процитировано

0

Ichthyoplankton temporal and spatial patterns in sub-Antarctic coastal and oceanic waters of the Southwestern Atlantic DOI
Daniel O. Bruno,

Luciana Riccialdelli,

Yamila A. Becker

и другие.

Estuarine Coastal and Shelf Science, Год журнала: 2025, Номер unknown, С. 109260 - 109260

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

The Sub-Antarctic Beagle Channel marine ecosystem: A regional and comprehensive sentinel of global change DOI
Gustavo Ferreyra, Humberto E. González

Journal of Marine Systems, Год журнала: 2023, Номер 241, С. 103914 - 103914

Опубликована: Июнь 30, 2023

Язык: Английский

Процитировано

1

Spring abundance, biomass and sizes of appendicularians between open sea MPA Namuncurá/ Burdwood Bank and the adjacent coastal area, Southwest Atlantic Ocean: Are they a key link in the trophic web? DOI Creative Commons
Nadia M. Alves,

Mariela L. Spinelli,

Jacobo Martín

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июнь 21, 2024

Abstract Appendicularians are recognized as one of the most abundant mesozooplankton groups in numerous pelagic environments and they play a crucial role marine ecosystems bridge gap between primary producers higher trophic levels food webs. Zooplankton samples were collected during three spring oceanographic surveys conducted 2014, 2015, 2016, coastal zone (Staten Island) oceanic (Namuncurá-Banco Burdwood Marine Protected Area). Our study focuses on comparative analysis species composition, density, biomass, maturity stages appendicularia these regions, which marked by distinct physical biological attributes. Two appendicularians found area, Oikopleura fusiformis Fritillaria borealis, former being dominant. Their distribution was different because F. borealis mainly concentrated while O. consistently recorded both zones. Chlorophyll-a concentrations to be than zone. These accompanied densities that area. The surface current velocity seems reflect total phytoplankton biomass On other hand, temperature for protected area lower could related larger sizes Baseline data areas surrounding is essential contribute stakeholders advise future changes translate into regional global processes.

Язык: Английский

Процитировано

0

A fast-track, high-throughput screening platform for biological nitrification inhibitors discovery based on soil relevant ammonia oxidizing strains DOI Open Access

Alexandros E. Kanellopoulos,

Andrea Malits, Hugo Ribeiro

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 3, 2024

Abstract Nitrogen cycling is critical for ecosystem functioning, with nitrification playing a central role. Excessive nitrification, triggered by heavy nitrogen fertilisation, contributes to reduced use efficiency, nitrate leaching, and nitrous oxide emissions. While synthetic inhibitors (SNIs) help reduce these impacts, their erratic performance environmental risks have shifted focus biological (BNIs) as sustainable alternatives. In vitro bioassays ammonia-oxidising microorganisms (AOM) are valuable BNI discovery research, but often low throughput rely on limited number of mostly non-soil-relevant or genetically modified ammonia-oxidizing bacteria (AOB) strains, lacking validation established BNIs. We present refined fast-track, high-throughput assay screening, utilizing soil-relevant, ecophysiologically phylogenetically diverse AOB ( Nitrosospira multiformis, Nitrosomonas ureae, communis ) archaea (AOA) strains Nitrososphaera viennensis, “ Ca . Nitrosocosmicus franklandianus”), achieving consistent cellular activity density. The was validated SNIs BNIs, showing differences in inhibition efficacy strain sensitivity, literature. As proof concept, root exudates from wheat genotypes were screened, demonstrating distinct profiles. proposed system advances previously available screening systems and, when integrated realistic soil tests, will facilitate the novel BNIs BNI-producing plant genotypes.

Язык: Английский

Процитировано

0