The Evolutionary Landscape of tRNA Modifications in Archaea: Insights from High-Throughput Sequencing

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Май 7, 2025

Abstract Transfer RNA (tRNA) modifications play essential roles in structure, stability and decoding accuracy, yet the evolutionary dynamics diversity of these remain incompletely understood. Recent advances high-throughput sequencing, including Ordered Two-Template Relay sequencing (OTTR-seq), now facilitate systematic, transcriptome-wide detection tRNA at single-base resolution. Here we employed OTTR-seq to comprehensively profile across nine archaeal species spanning diverse ecological niches, thermophiles, methanogens, acidophiles, halophiles. We identified coordinated mutually exclusive methylation patterns acceptor stem position 6 67 hyperthermophiles, as well unexpected clade-specific co-modifications core positions 10 26, demonstrating tolerance canonical anti-determinants Thermoprotei. Comparative genomic analyses revealed clear associations between changes enzyme domain architectures, divergence homologs Trm14, Trm10, Trm11, Trm1. also expanded upon previously characterized identity elements D-stem, such conserved G10oU25 pair critical for Trm11 specificity, providing additional insight into determinants that likely govern enzyme-substrate interactions. Together, findings offer valuable insights modification biology dynamics, provide a foundation guiding targeted biochemical experiments mechanistic studies.

Язык: Английский

Decoding tRNA Modifications: Integrating Molecular, Biological, and Pathological Insights

Journal of Molecular Biology, Год журнала: 2025, Номер unknown, С. 169238 - 169238

Опубликована: Май 1, 2025

Язык: Английский