Development and Evaluation of a New Measles Detection Assay Using Real-Time RT-PCR
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(5), С. 1801 - 1801
Опубликована: Фев. 20, 2025
The
severity
of
MeV
infection
has
been
greatly
reduced
by
the
development
a
live
attenuated
vaccine,
which
incorporated
into
vaccination
programs
in
many
countries.
However,
poor
access
to
health
facilities,
and
above
all,
increase
anti-vaccination
movements,
prevented
achievement
sufficient
coverage.
In
outbreak
scenarios,
rapid
transportable
method
can
improve
differential
diagnosis,
including
removing
ambiguity
suspected
measles
cases,
contacts,
or
cohort.
response
need,
we
have
developed
new
RT-qPCR-based
detection
assay.
LOD
assay
was
determined
on
different
PCR
machines
higher
threshold
1-1.2
103
copies/mL.
joint
diagnostic
sensitivity
ELISA
RT-PCR
(used
together)
100%,
used
combinedly,
these
two
methods
enable
all
measles-infected
persons,
is
extremely
important
for
controlling
contagion
spread
infection.
During
clinical
validation
200
samples
from
measles-suspected
cases
using
ELISA,
157
showed
positive
result,
while
163
were
confirmed
RT-qPCR
concordance
between
techniques
93%.
According
our
results,
real-time
approach
study
more
sensitive
appears
be
promising
diagnosis
during
early
stages
disease,
likely
before
rise
specific
IgM
antibodies
detected
ELISA.
Язык: Английский
Detainees within immigration removal centres: a highly unique cohort of dental patients
Deleted Journal,
Год журнала:
2025,
Номер
2(1)
Опубликована: Фев. 21, 2025
Язык: Английский
Seroprevalence of Measles Antibodies Among Migrant Populations: A Systematic Review and Meta-Analysis
Cureus,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 22, 2024
Incomplete
or
interrupted
vaccination
schedules
put
migrant
communities
at
higher
risk
for
measles,
which
remains
a
serious
public
health
concern.
The
objective
of
this
systematic
review
was
to
evaluate
the
pooled
seroprevalence
measles
antibodies
among
groups
globally
and
offer
data
guide
initiatives.
Our
literature
search
included
PubMed,
Scopus,
Embase
databases,
covering
publications
from
1990
2023,
systematically
refined
using
specific
inclusion
exclusion
criteria.
Only
observational
studies
documenting
antibody
defined
were
ensure
relevance
quality
in
addressing
study's
objective.
Meta-analytical
techniques
such
as
random-effects
models
employed
assess
seroprevalence.
meta-analysis
comprised
36
with
42,972
participants
total.
Among
communities,
overall
83%
(95%CI:
80%-87%).
Studies
showed
significant
heterogeneity
(I
Язык: Английский