Insights into the Role of LncRNAs and miRNAs in Glioma Progression and Their Potential as Novel Therapeutic Targets

Cancers, Год журнала: 2023, Номер 15(13), С. 3298 - 3298

Опубликована: Июнь 22, 2023

Accumulating evidence supports that both long non-coding and micro RNAs (lncRNAs miRNAs) are implicated in glioma tumorigenesis progression. Poor outcome of gliomas has been linked to late-stage diagnosis mostly ineffectiveness conventional treatment due low knowledge about the early stage gliomas, which not possible observe with diagnostic approaches. The past few years witnessed a revolutionary advance biotechnology neuroscience understanding tumor-related molecules, including involved angiogenesis progression cells thus used as prognostic biomarkers well novel therapeutic targets. emerging research on lncRNAs miRNAs highlights their crucial role progression, offering new insights into disease. These hold significant potential targets, paving way for innovative approaches against glioma. This review encompasses comprehensive discussion gene regulation is responsible promotion or inhibition collects existing links between these key cancer-related molecules.

Язык: Английский

---

MUC1 and MUC4 expression are inversely correlated and trigger immunological response and transport pathways in adult-type diffuse gliomas

Computers in Biology and Medicine, Год журнала: 2025, Номер 187, С. 109730 - 109730

Опубликована: Янв. 31, 2025

Язык: Английский

---

Profiling the molecular and clinical landscape of glioblastoma utilizing the Oncology Research Information Exchange Network brain cancer database

Neuro-Oncology Advances, Год журнала: 2024, Номер 6(1)

Опубликована: Янв. 1, 2024

Abstract Background Glioblastoma exhibits aggressive growth and poor outcomes despite treatment, its marked variability renders therapeutic design prognostication challenging. The Oncology Research Information Exchange Network (ORIEN) database contains complementary clinical, genomic, transcriptomic profiling of 206 glioblastoma patients, providing opportunities to identify novel associations between molecular features clinical outcomes. Methods Survival analyses were performed using the Logrank test, evaluated Wilcoxon chi-squared tests with q-values derived via Benjamini-Hochberg correction. Mutational utilized sample-level enrichments from whole exome sequencing data, statistical one-sided Fisher Exact test Transcriptomic a student’s t-test Expression fold changes processed Ingenuity Pathway Analysis determine pathway-level alterations groups. Results Key findings include an association MUC17, SYNE1, TENM1 mutations prolonged overall survival (OS); decreased OS associated higher epithelial factor receptor (EGFR) mRNA expression, but not EGFR amplification or mutation; 14-transcript signature > 2 years; transcripts < 1 year. Conclusions Herein, we report first analysis ORIEN cases, incorporating sample reclassification under updated 2021 diagnostic criteria. These create multiple avenues for further investigation reinforce value multi-institutional consortia such as in deepening our knowledge intractable diseases glioblastoma.

Язык: Английский

---

Genomic characterization and risk stratification of esophageal squamous dysplasia

Medical Review, Год журнала: 2024, Номер 4(3), С. 244 - 256

Опубликована: Май 14, 2024

The majority of esophageal squamous dysplasia (ESD) patients progress slowly, while a subset can undergo recurrence rapidly or to invasive cancer even after proper treatment. However, the molecular mechanisms underlying these clinical observations are still largely unknown.

Язык: Английский

---

MUC1 and MUC4 expression are inversely correlated and trigger immunological response and transport pathways in gliomas

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 30, 2024

Abstract Diffuse gliomas are tumors that arise from glial or progenitor cells. They currently classified as astrocytoma isocitrate dehydrogenase (IDH)-mutant oligodendroglioma IDH-mutant, and chromosome arms 1p/19q-codeleted, both slower-growing tumors, glioblastoma (GBM), a more aggressive tumor. Despite advances in the diagnosis treatment of gliomas, median survival time after GBM remains low, approximately 15 months, with 5-year overall rate only 6.8%. Therefore, new biomarkers therapy targets could support better prognosis these would be great value. MUC1 MUC4, membrane-bound mucins, has been identified potential biomarker several tumors. However, role mucins adult not yet well explored. Here, we show for first time, retrospective study by silico analysis, relevance correlation genes gliomas. Analysis diffuse glioma patient cohorts revealed differential methylation expression patterns MUC4 across non-GBM subtypes. patients exhibited decreased elevated (r-0.25, p < 0.0001) whereas increased its lower (r-0.13, = 0.1344). Conversely, patients, showed higher levels low (r-0.27, high (r-0.32, 0.0001). The influenced (OS) (p 0.0344), associated worse OS. correlated MUC20 (r 0.54) 0.53) Functional enrichment analysis distinct biological roles co-expressed involvement innate immunity, antigen processing, pro-inflammatory responses cohorts, integrin-based signaling pathways patients. were involved ion transport Using molecular docking, observed domains physically capable interacting immune response-related proteins such Receptor Advanced Glycation End-products (RAGE), Major Histocompatibility Complex II (MHCII), extracellular matrix receptor integrin alpha 2 (ITGA2). These findings shed light on mechanisms underlying progression highlight prognostic markers therapeutic management.

Язык: Английский

---

Overexpression of EZH2 is associated with clinicopathological parameters and poor prognosis in gliomas

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Апрель 30, 2024

Histone methyltransferase EZH2, primarily localized in the nucleus, mediates constitutive Polycomb repressive complex activity by trimethylating lysine 27 of histone H3 (H3K27me3), leading to gene silencing through canonical and noncanonical mechanisms, resulting transcriptional repression or activation. Its involvement is crucial cell growth, proliferation, differentiation, apoptosis, with its effects linked regulation various targets signaling pathways. Overexpression EZH2 alters expression function, thereby facilitating cancer progression. Recent research has identified potential prognostic role glioma patients. This study assesses clinicopathological significance value gliomas using available data. The mRNA levels tumor tissues normal were assessed timer2.0 data from CGCA TGCA. was determined Kaplan-Meier plotter. A total 147 clinical samples patients underwent immunohistochemistry analysis evaluate protein expression. Cox proportional hazards regression model survival curves employed assess relationship between expression, parameters, overall (OS). Across multiple cohorts, found be upregulated amplified tissues. In high-grade patients, significantly increased, higher correlated poorer OS, disease-specific (DSS), progression-free interval (PFI). Therefore, level may serve as a biomarker for

Язык: Английский

---

Whole exome-seq and RNA-seq data reveal unique neoantigen profiles in Kenyan breast cancer patients

medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 20, 2024

ABSTRACT Background The immune response against tumors relies on distinguishing between self and non-self, the basis of cancer immunotherapy. Neoantigens from somatic mutations are central to many immunotherapeutic strategies understanding their landscape in breast is crucial for targeted interventions. We aimed profile neoantigens Kenyan patients using genomic DNA total RNA paired tumor adjacent non-cancerous tissue samples 23 patients. Methods sequenced genome-wide exome (WES) RNA, which were identified expression quantified, respectively. Neoantigen prediction focused human leukocyte antigens (HLA) cancer, HLA type I. alleles predicted WES data covering samples, identifying four that present at least 50% deemed potentially immunogenic if median IC50 binding scores ≤500nM expressed [transcripts per million (TPM) >1] samples. Results An average 1465 10260 genes had score >1 TPM presence significantly correlated with ( R 2 =0.570, P =0.001). Assessing 58 reported catalog (COSMIC, v99) be commonly mutated 44 (76%) produced >2 among patients, a mean 10.5 ranging 93. For genes, 477 putative identified, predominantly derived missense (88%), indels (6%), frameshift (6%). Notably, 78% patient-specific. HLA-C*06:01 allele was associated majority (194), followed by HLA-A*30:01 (131), HLA-A*02:01 (103), HLA-B*58:01 (49). Among interest MUC17 , TTN MUC16 AKAP9 NEB RP1L1 CDH23 PCDHB10 BRCA2 TP53 TG RB1 . Conclusions unique neoantigen profiles our patient group highlight potential immunotherapy personalized treatment as well biomarkers prognosis. producing these neoantigens, compared other populations, provide an opportunity validation much larger sample cohort.

Язык: Английский

---

Integrative Genomic and Transcriptomic Analysis in Acute Interstitial Pneumonia

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(23)

Опубликована: Дек. 1, 2024

ABSTRACT Acute Interstitial Pneumonia (AIP) represents a severe form of diffuse lung injury within the idiopathic interstitial pneumonia spectrum. Given limited understanding its molecular basis, this study aims to elucidate AIP's genomic and transcriptomic profiles uncover pathophysiological underpinnings identify potential therapeutic targets. We conducted comprehensive analysis data from tissues 15 AIP patients. This included assessing differentially expressed genes (DEGs) identifying mutations in exonic coding variants, as well analysing expression quantitative trait loci (eQTL) link non‐coding SNP genotypes with gene levels. Transcriptomic revealed significant upregulation linked Type I interferon receptor keratin filament, downregulation related focal adhesion endothelial integrity, compared healthy individuals. These patterns were distinct those observed pulmonary fibrosis (IPF) non‐IPF diseases (ILDs). Genomic highlighted associated extracellular matrix. Additionally, eQTL profiling provided insights into genetic regulation AIP. Our findings reveals unique landscape, differentiating it other ILDs laying groundwork for future diagnostic research.

Язык: Английский

---

Mucins as Precision Biomarkers in Glioma: Emerging Evidence for Their Potential in Biospecimen Analysis and Outcome Prediction

Biomedicines, Год журнала: 2024, Номер 12(12), С. 2806 - 2806

Опубликована: Дек. 11, 2024

Despite attempts at improving survival by employing novel therapies, progression in glioma is nearly universal. Precision biomarkers are critical to advancing outcomes; however, for currently unknown. Most data on which the field can draw biomarker identification comprise tissue-based analysis requiring biospecimen be removed from tumor. Non-invasive specimen-based precision needed. Mucins captured tissue and blood increasingly studied cancer, with several studies exploring their role as detect disease monitor progression. CA125, also known MUC16, implemented a clinic ovarian cancer. Similarly, mucins membrane-bound, facilitating downstream signaling associated tumor resistance hallmarks of Evidence supports mucin expression cells relationships detection, progression, resistance, patient outcomes. The differential across tissues organs could provide means attributing signals measured serum or plasma. In this review, we compiled existing research candidate glioma, focusing promising relationship leading framework biospecimens well avenues validation evolve.

Язык: Английский

---

Whole exome-seq and RNA-seq data reveal unique neoantigen profiles in Kenyan breast cancer patients

Frontiers in Oncology, Год журнала: 2024, Номер 14

Опубликована: Дек. 11, 2024

The immune response against tumors relies on distinguishing between self and non-self, the basis of cancer immunotherapy. Neoantigens from somatic mutations are central to many immunotherapeutic strategies understanding their landscape in breast is crucial for targeted interventions. We aimed profile neoantigens Kenyan patients using genomic DNA total RNA paired tumor adjacent non-cancerous tissue samples 23 patients.

Язык: Английский

---