Diagnostics,
Год журнала:
2023,
Номер
13(13), С. 2219 - 2219
Опубликована: Июнь 29, 2023
The
emergence
of
COVID-19
has
caused
unprecedented
impacts
on
global
public
health
and
many
other
aspects.
Meanwhile,
types
methods
have
been
developed
to
detect
the
causative
agent,
SARS-CoV-2;
this
greatly
advanced
technologies
in
diagnostic
field.
Here,
we
describe
development
validation
a
sample-in-result-out
POCKIT
Central
SARS-CoV-2
PCR
system
for
detecting
comparison
with
commercial
reference
real-time
RT-PCR
assay
(TaqPath
Combo
Kit).
Both
assays
were
specific
did
not
cross-react
non-SARS-CoV-2
agents.
able
various
strains
including
some
variants.
Based
testing
serial
dilutions
USA-WA1/2020
isolate,
limit
detection
was
0.8
TCID50/mL
(1.87
×
103
genomic
copies/mL)
0.16
(3.75
102
PCR.
Subsequently,
183
clinical
samples
tested
by
both
sensitivity,
specificity,
agreement
91.7%,
100%,
94.0%,
respectively,
when
compared
compact
sample-to-result
is
simplified
efficient
point-of-care
tool
detection.
In
addition,
platform
can
be
readily
adapted
human
animal
viruses.
Chemical Physics Impact,
Год журнала:
2023,
Номер
8, С. 100414 - 100414
Опубликована: Дек. 5, 2023
In
recent
years,
researchers
have
made
significant
contributions
to
the
field
of
Surface
Plasmon
Resonance
sensors
and
their
potential
applications.
SPR
features
include
real-time
monitoring
biomolecular
interactions,
label-free
detection,
high
sensitivity,
ability
analyze
multiple
samples
at
same
time.
These
characteristics
make
biosensors
a
valuable
tool
in
pharmaceutical
industry
for
ensuring
drug
efficacy
safety.
Furthermore,
reputation
providing
quantitative
qualitative
superior
performance
applications
such
as
development,
environmental
monitoring,
food
safety,
medical
diagnostics,
clinical
diagnosis,
biological
investigations,
biomolecule
interactions.
This
review
will
go
over
advances
how
they
can
be
used
discovery
development.
We
also
look
challenges
opportunities
using
industry.
Point-of-care
(POC)
nanosensors
with
high
screening
efficiency
show
promise
for
user-friendly
manipulation
in
the
ever-increasing
on-site
analysis
demand
illness
diagnosis,
environmental
monitoring,
and
food
safety.
Currently,
inspired
by
merits
of
integrating
advanced
nanomaterials,
molecular
biology,
machine
learning,
artificial
intelligence,
lateral
flow
immunoassay
(LFIA)-based
POC
have
been
devoted
to
satisfying
commercial
demands
terms
sensitivity,
specificity,
practicality.
Herein,
we
examine
use
multidimensional
enhanced
LFIA
various
fields
over
past
two
decades,
focusing
on
introducing
nanomaterials
improve
acquisition
capability
small
order
magnitude
targets
through
engineering
transformations
emphasizing
interdomain
fusion
collaboratively
address
inherent
challenges
current
applications,
such
as
multiplexing,
development
detectors
quantitative
analysis,
more
practical
sensitivity
enhancement.
Specifically,
this
comprehensive
review
encompasses
latest
advances
comprehending
an
alternative
signal
transduction
pattern,
aiming
achieve
rapid,
ultrasensitive,
"sample-to-answer"
available
options
progressive
applications
nanosensors.
In
summary,
cross-collaboration
disciplines,
has
potential
break
barriers
toward
commercialization
laboratory-level
nanosensors,
thus
leading
emergence
next
generation
LFIA.
Abstract
With
the
development
of
technology,
biosensors
are
increasingly
used
in
biomedical
field.
Due
to
high
sensitivity
optical
signals,
good
stability,
signal‐to‐noise
ratio,
and
different
spectral
characteristics
analytes,
can
achieve
direct
real‐time
detection
analytes
with
specificity
compared
traditional
analytical
techniques.
In
view
rapid
for
vivo
applications
their
promising
future,
we
have
attempted
summarize
working
principles
recent
advances
application
humans,
aim
helping
readers
gain
an
in‐depth
detailed
understanding
developed
years
biological
living
body.
this
review,
focus
on
some
current
widely
biosensors,
including
colorimetric
fluorescence
surface‐enhanced‐Raman‐scattering‐based
biosensors.
The
each
type
biosensor
concisely
described
body
is
summarized
by
category.
We
conclude
looking
at
prospects
AIMS Microbiology,
Год журнала:
2023,
Номер
9(2), С. 375 - 401
Опубликована: Янв. 1, 2023
Over
the
last
three
years,
after
outbreak
of
COVID-19
pandemic,
an
unprecedented
number
novel
diagnostic
tests
have
been
developed.
Assays
to
evaluate
immune
response
SARS-CoV-2
widely
considered
as
part
control
strategy.
The
lateral
flow
immunoassay
(LFIA),
detect
both
IgM
and
IgG
against
SARS-CoV-2,
has
studied
a
point-of-care
(POC)
test.
Compared
laboratory
tests,
LFIAs
are
faster,
cheaper
user-friendly,
thus
available
also
in
areas
with
low
economic
resources.
Soon
onset
numerous
kits
for
rapid
antibody
detection
were
put
on
market
emergency
use
authorization.
However,
since
then,
scientists
tried
better
define
accuracy
these
their
usefulness
different
contexts.
In
fact,
while
during
first
phase
pandemic
auxiliary
molecular
diagnosis
COVID-19,
successively
became
tool
seroprevalence
surveillance
address
infection
policies.
When
2021
massive
vaccination
campaign
was
implemented
worldwide,
interest
LFIA
reemerged
due
need
establish
extent
longevity
immunization
vaccinated
population
priorities
guide
health
policies
low-income
countries
limited
access
vaccines.
Here,
we
summarize
accuracy,
advantages
limits
POC
detection,
highlighting
efforts
that
made
improve
this
technology
over
few
years.
Biosensors,
Год журнала:
2022,
Номер
12(12), С. 1101 - 1101
Опубликована: Дек. 1, 2022
A
new
transmission
route
of
SARS-CoV-2
through
food
was
recently
considered
by
the
World
Health
Organization
(WHO),
and,
given
pandemic
scenario,
search
for
fast,
sensitive,
and
low-cost
methods
is
necessary.
Biosensors
have
become
a
viable
alternative
large-scale
testing
because
they
overcome
limitations
standard
techniques.
Herein,
we
investigated
ability
gold
spherical
nanoparticles
(AuNPs)
functionalized
with
oligonucleotides
to
detect
demonstrated
their
potential
be
used
as
plasmonic
nanobiosensors.
The
loop-mediated
isothermal
amplification
(LAMP)
technique
amplify
viral
genetic
material
from
raw
virus-containing
solution
without
any
preparation.
detection
virus
presence
or
absence
performed
ultraviolet–visible
(UV–Vis)
absorption
spectroscopy,
monitoring
band
surface
resonance
(SPR)
AuNPs.
displacement
peak
525
nm
AuNPs
indicated
(particular
region
gold).
On
other
hand,
~300
when
RNA
bound
nanobiosensor
system
designed
N
gene
in
dynamic
concentration
range
0.1
50
×
103
ng·mL−1
limit
(LOD)
1
(2.7
copy
per
µL),
indicating
excellent
sensitivity.
applied
on
surfaces
vegetables
showed
100%
accuracy
compared
quantitative
reverse
transcription
polymerase
chain
reaction
(RT-qPCR)
technique.
Therefore,
selective,
simple,
providing
rapid
ready-to-eat
vegetables.
Analytical Methods,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Reviews
the
development
of
visual
detection
methods
in
recent
years
based
on
different
Cas
and
analyzes
their
advantages
disadvantages
as
well
challenges
future
research.
Biosensors,
Год журнала:
2023,
Номер
13(6), С. 640 - 640
Опубликована: Июнь 9, 2023
Viruses
are
widespread
in
the
environment,
and
many
of
them
major
pathogens
serious
plant,
animal,
human
diseases.
The
risk
pathogenicity,
together
with
capacity
for
constant
mutation,
emphasizes
need
measures
to
rapidly
detect
viruses.
highly
sensitive
bioanalytical
methods
diagnose
monitor
socially
significant
viral
diseases
has
increased
past
few
years.
This
is
due,
on
one
hand,
incidence
general
(including
unprecedented
spread
a
new
coronavirus
infection,
SARS-CoV-2),
and,
other
overcome
limitations
modern
biomedical
diagnostic
methods.
Phage
display
technology
antibodies
as
nano-bio-engineered
macromolecules
can
be
used
sensor-based
virus
detection.
review
analyzes
commonly
detection
approaches
shows
prospects
use
prepared
by
phage
sensing
elements
