Diagnostics,
Год журнала:
2023,
Номер
13(13), С. 2219 - 2219
Опубликована: Июнь 29, 2023
The
emergence
of
COVID-19
has
caused
unprecedented
impacts
on
global
public
health
and
many
other
aspects.
Meanwhile,
types
methods
have
been
developed
to
detect
the
causative
agent,
SARS-CoV-2;
this
greatly
advanced
technologies
in
diagnostic
field.
Here,
we
describe
development
validation
a
sample-in-result-out
POCKIT
Central
SARS-CoV-2
PCR
system
for
detecting
comparison
with
commercial
reference
real-time
RT-PCR
assay
(TaqPath
Combo
Kit).
Both
assays
were
specific
did
not
cross-react
non-SARS-CoV-2
agents.
able
various
strains
including
some
variants.
Based
testing
serial
dilutions
USA-WA1/2020
isolate,
limit
detection
was
0.8
TCID50/mL
(1.87
×
103
genomic
copies/mL)
0.16
(3.75
102
PCR.
Subsequently,
183
clinical
samples
tested
by
both
sensitivity,
specificity,
agreement
91.7%,
100%,
94.0%,
respectively,
when
compared
compact
sample-to-result
is
simplified
efficient
point-of-care
tool
detection.
In
addition,
platform
can
be
readily
adapted
human
animal
viruses.
Journal of AOAC International,
Год журнала:
2024,
Номер
107(3), С. 519 - 528
Опубликована: Фев. 3, 2024
Abstract
Since
2019
the
world
has
been
in
a
combat
with
highly
contagious
disease
COVID-19
which
is
caused
by
rapid
transmission
of
SARS-CoV-2
virus
(severe
acute
respiratory
syndrome
coronavirus
2).
Detection
this
an
early
stage
helps
to
control
its
spread
and
management.
To
epidemic
one-time
effective
medication,
improved
quick
analytical
procedures
must
be
developed
validated.
The
requirement
for
accurate
precise
methods
diagnosis
antibodies
infected
patients
matter
concern.
global
impact
motivated
scientists
researchers
investigate
develop
various
diagnostic
techniques.
This
review
includes
study
standard
are
reliable
accredited
recognition
said
virus.
For
detection
RNA,
RT-PCR
(Real-time
reverse
transcriptase-polymerase
chain
reaction)
method
among
other
and,
thus,
considered
as
“gold
standard”
technique.
Here,
we
outline
most
extensively
used
diagnosing
COVID-19,
along
brief
description
each
technique
aspects/perspective.
A
bifunctional
ensemble
of
papain-capped
gold
nanoparticles
(AuNFs)
exhibits
dual
functionality,
manifesting
peroxidaselike
and
4-nitrophenol
reduction
activities.
The
AuNFs,
possessing
an
average
size
approximately
82.27
±
1.95
nm,
are
synthesized
under
ambient
conditions
through
the
mediation
papain,
wherein
HAuCl4
by
ascorbic
acid
is
facilitated.
These
nanoflowers
capable
catalyzing
H2O2
to
oxidize
target
3,3,5,5-tetramethylbenzidine,
resulting
in
production
a
visual
color
blue.
Therefore,
simple
method
providing
sensitivity
0.44
µM
(signal/noise
ratio
=
2)
linearity
within
range
0
40
available
detect
colorimetry.
Subsequently,
chemical
activity
associated
with
4-NP
(4-nitrophenol)
was
studied,
it
found
exhibit
catalysis
rate
0.29
min-1
,
surpassing
that
alternative
catalysts.
findings
underscore
potential
utility
our
AuNFs
catalytic
biosensing
applications.
Diagnostics,
Год журнала:
2023,
Номер
13(13), С. 2219 - 2219
Опубликована: Июнь 29, 2023
The
emergence
of
COVID-19
has
caused
unprecedented
impacts
on
global
public
health
and
many
other
aspects.
Meanwhile,
types
methods
have
been
developed
to
detect
the
causative
agent,
SARS-CoV-2;
this
greatly
advanced
technologies
in
diagnostic
field.
Here,
we
describe
development
validation
a
sample-in-result-out
POCKIT
Central
SARS-CoV-2
PCR
system
for
detecting
comparison
with
commercial
reference
real-time
RT-PCR
assay
(TaqPath
Combo
Kit).
Both
assays
were
specific
did
not
cross-react
non-SARS-CoV-2
agents.
able
various
strains
including
some
variants.
Based
testing
serial
dilutions
USA-WA1/2020
isolate,
limit
detection
was
0.8
TCID50/mL
(1.87
×
103
genomic
copies/mL)
0.16
(3.75
102
PCR.
Subsequently,
183
clinical
samples
tested
by
both
sensitivity,
specificity,
agreement
91.7%,
100%,
94.0%,
respectively,
when
compared
compact
sample-to-result
is
simplified
efficient
point-of-care
tool
detection.
In
addition,
platform
can
be
readily
adapted
human
animal
viruses.
