Green micellar liquid chromatography for The simultaneous determination of triple antiretroviral therapy agents in pharmaceutical Formulation, A sustainable approach

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113400 - 113400

Опубликована: Март 1, 2025

Язык: Английский

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Greener and Whiter Analytical Procedure for Theobromine and Caffeine Determination in Tea Using Dimethyl Carbonate as an Extraction Solvent and Mobile Phase Constituent in Reversed-Phase Liquid Chromatography

ACS Omega, Год журнала: 2025, Номер 10(12), С. 12432 - 12440

Опубликована: Март 24, 2025

For the first time, dimethyl carbonate was applied as sole organic solvent, both an eluent in solid-phase extraction (SPE) and for simultaneous determination of theobromine caffeine tea extracts using reversed-phase ultrahigh-performance liquid chromatography (RP-UHPLC). The proposed procedure has been validated complies with requirements green analytical chemistry (GAC). Solid-phase used to purify samples, achieving high recovery rates (97–101%) precision (RSD < 2%). method showed satisfactory sensitivity (achieving limit quantitation values 0.2 ng 0.35 caffeine), although it poorer compared developed conventional HPLC methanol, where reached 0.045 0.05 ng, respectively. low toxicity minimal solvent consumption DMC contributed a much more beneficial environmental profile method, confirmed by AGREEprep, AGREE, ChlorTox evaluations. Moreover, switching from UHPLC, remaining principles GAC were further incorporated reducing consumption, analysis energy requirements. In addition, RGB tool highlighted higher performance while not compromising quality or economic practicality. robustness benefits make this viable alternative routine ingredients, supporting sustainable practices.

Язык: Английский

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Ultrasound-assisted green synthesis of silver nanoparticles using Ruta graveolens L. Extract and antitumor evaluation

Ultrasonics Sonochemistry, Год журнала: 2025, Номер unknown, С. 107340 - 107340

Опубликована: Апрель 1, 2025

This study aims to evaluate the ultrasound-assisted synthesis of silver nanoparticles (AgNPs) based on an aqueous extract Ruta graveolens L. reduce Ag+ Ag0. In addition, biological activity synthesized AgNPs was evaluated against tumor cells. The following parameters were for synthesis: proportion between solution (0.1 mol L-1 Ag+) and R. extract, pH (5, 7, 9), temperature containing ultrasound (US) type (bath probes), US as frequency (37, 80 kHz bath 20 amplitude time application US. order confirm effect, "silent" experiments (without US) performed. Using optimized conditions (US bath, 1 + 5, v v-1, kHz, 70 % amplitude, °C, 9, 25 min sonication time) it possible obtain mean size, PI, zeta potential 30 nm, 0.129, -34.44 mV, respectively. For comparison results, in condition presented 66 0.412, -22.12 lower, more uniform, stable when compared with magnetic stirring. morphology using predominantly spherical monodisperse. cell lines HaCat (keratinocytes), L929 (fibroblasts), B16-F10 (melanoma) different antioxidant measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. A higher cytotoxic effect melanoma line (IC50 = 2.12 µg mL-1) normal good result found DPPH assay, IC50 234.3 mL-1 free radical scavenging. Therefore, technology presents a promising sustainable green method avoiding use toxic reagents obtained showed potent anticancer activity.

Язык: Английский

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Guidelines on the proper selection of greenness and related metric tools in analytical chemistry – a tutorial

Analytica Chimica Acta, Год журнала: 2025, Номер unknown, С. 344052 - 344052

Опубликована: Апрель 1, 2025

Язык: Английский

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Preconcentration of selected cephalosporins using waste PET-derived UIO-66 as an adsorbent before HPLC-DAD quantification

Green Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 100272 - 100272

Опубликована: Апрель 1, 2025

Язык: Английский

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