Changes in the Phenotype and Metabolism of Peritoneal Macrophages in Mucin-2 Knockout Mice and Partial Restoration of Their Functions In Vitro After L-Fucose Treatment DOI Open Access
Elena Arzhanova,

Yulia Makusheva,

Elena G. Pershina

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 26(1), С. 13 - 13

Опубликована: Дек. 24, 2024

In the development of inflammatory bowel disease (IBD), peritoneal macrophages contribute to resident intestinal macrophage pool. Previous studies have demonstrated that oral administration L-fucose exerts an immunomodulatory effect and repolarizes in vivo mice. this study, we analyzed phenotype metabolic profile from Muc2−/− mice, as well on morphological characteristics these vitro. The investigation utilized flow cytometry, quantitative PCR (qPCR), measurement intracellular ATP Ca2+ concentrations, analysis mitochondrial respiration membrane potential, transmission electron microscopy (TEM) for ultrastructural evaluations. mice exhibited lower levels their macrophages, a higher percentage stellate increased oxygen consumption rate (OCR), combined with mitochondria displaying abnormal ultrastructure. Additionally, there was five-fold increase condensed compared level C57BL/6 number CD209+ reduced three-fold, while M1-like cells two-fold treatment enhanced production expression Parp1, Mt-Nd2, Mt-Nd6 genes, which may suggest reduction pro-inflammatory factor shift differentiation towards M2 phenotype.

Язык: Английский

Mitochondrial DNA copy number and its association with venous thromboembolism in patients with cancer DOI Creative Commons

Rafaela Vostatek,

Marina Trappl,

Cornelia Englisch

и другие.

Thrombosis Research, Год журнала: 2025, Номер 248, С. 109285 - 109285

Опубликована: Фев. 13, 2025

Язык: Английский

Процитировано

0
Bakuchiol mitigates colitis through GPR120 activation DOI
Fangfang Xu, Jixia Wang, Tianyu Zhang

и другие.

Phytomedicine, Год журнала: 2025, Номер 140, С. 156618 - 156618

Опубликована: Март 6, 2025

Язык: Английский

Процитировано

0
Changes in the Phenotype and Metabolism of Peritoneal Macrophages in Mucin-2 Knockout Mice and Partial Restoration of Their Functions In Vitro After L-Fucose Treatment DOI Open Access
Elena Arzhanova,

Yulia Makusheva,

Elena G. Pershina

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 26(1), С. 13 - 13

Опубликована: Дек. 24, 2024

In the development of inflammatory bowel disease (IBD), peritoneal macrophages contribute to resident intestinal macrophage pool. Previous studies have demonstrated that oral administration L-fucose exerts an immunomodulatory effect and repolarizes in vivo mice. this study, we analyzed phenotype metabolic profile from Muc2−/− mice, as well on morphological characteristics these vitro. The investigation utilized flow cytometry, quantitative PCR (qPCR), measurement intracellular ATP Ca2+ concentrations, analysis mitochondrial respiration membrane potential, transmission electron microscopy (TEM) for ultrastructural evaluations. mice exhibited lower levels their macrophages, a higher percentage stellate increased oxygen consumption rate (OCR), combined with mitochondria displaying abnormal ultrastructure. Additionally, there was five-fold increase condensed compared level C57BL/6 number CD209+ reduced three-fold, while M1-like cells two-fold treatment enhanced production expression Parp1, Mt-Nd2, Mt-Nd6 genes, which may suggest reduction pro-inflammatory factor shift differentiation towards M2 phenotype.

Язык: Английский

Процитировано

0