Novel Charge-Switch Derivatization Method Using 3-(Chlorosulfonyl)benzoic Acid for Sensitive RP-UHPLC/MS/MS Analysis of Acylglycerols, Sterols, and Prenols

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

Chemical derivatization involves the reaction of an analyte with a agent to modify its structure, improving peak shape, chromatographic performance, structural analysis, ionization efficiency, and sensitivity. A novel method using 3-(chlorosulfonyl)benzoic acid is developed for determination monoacylglycerols, diacylglycerols, free sterols, tocopherols reversed-phase ultra-high-performance liquid chromatography–tandem mass spectrometry (RP-UHPLC/MS/MS) in negative ion mode. The spectrometric properties derivatized lipids are investigated by 29 lipid standards spanning four classes. process optimized pooled plasma spiked 9 internal standards, achieving optimal yield time 40 min at 60 °C. stability derivatives confirmed, short-term maintained 10 h 4 °C long-term preserved 5 days −80 repeatability reproducibility verified one/two operator(s), which underscores simplicity robustness method, calibration curves high linear regression coefficients illustrate accuracy method. approach, combines RP-UHPLC/MS/MS use specific fragmentation patterns, significantly reduces limits detection, reaching 15–25 pmol/mL sterols plasma. applied analysis human plasma, leading identification 92 species targeted This represents substantial improvement sensitivity detection capabilities compared those previously reported methods.

Язык: Английский

Versatile and reliable extraction of phytosterols employing sonochemical synthesized molecularly imprinted polymer

Journal of Chromatography Open, Год журнала: 2024, Номер 6, С. 100174 - 100174

Опубликована: Сен. 10, 2024

Язык: Английский