Chitosan decorated oleosomes loaded propranolol hydrochloride hydrogel repurposed for Candida albicans -vaginal infection

Nanomedicine, Год журнала: 2024, Номер 19(15), С. 1369 - 1388

Опубликована: Июнь 20, 2024

Our investigation aims to estimate the antifungal effect of propranolol hydrochloride (PNL).

Язык: Английский

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In vitro and preclinical evaluation of the antifungal activity of 6-methoxy-1 H-indole-2-carboxylic acid produced by Bacillus toyonensis strain OQ071612 formulated as nanosponge hydrogel

Microbial Cell Factories, Год журнала: 2025, Номер 24(1)

Опубликована: Апрель 1, 2025

Abstract Background In a previous study, 6-methoxy-1 H-indole-2-carboxylic acid (MICA) was isolated from the culture broth of Bacillus toyonensis strain OQ071612 soil isolate in our laboratory, and it demonstrated promising antifungal activities. The current study designed to create nanosponge (NS)-hydrogel (HG)-containing MICA followed by vitro preclinical evaluation for potential clinical use topical treatment mycotic infections. Results enhanced NS formula created using Box Behnken Design (BBD), with independent process parameters including polyvinyl alcohol percentage (w/v%), homogenization time, speed polymer: linker ratio. Dependent were particle size (PS), polydispersity index (PDI), entrapment efficiency percent (EE%). A hydrogel formulated NS. drug release data indicated that best matched Higuchi’s kinetic model. NS-HG stable when compared fluconazole, exhibited increased antimycotic activity against C. albicans . An vivo investigation revealed MICA-NS-HG survival rates, wound gap repair, reduction, inflammation inhibition. Masson’s trichrome staining histological analyses collagen deposition improved healing. Moreover, 1.5-fold greater permeability through rat skin control, 1% isoconazole. Conclusion formulation is viable vehicle better more effective MICA. These findings represent significant advancement derived naturally occurring bacteria.

Язык: Английский

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Enhanced wound healing potential of arabincoside B isolated from Caralluma Arabica in rat model; a possible dressing in veterinary practice

BMC Veterinary Research, Год журнала: 2024, Номер 20(1)

Опубликована: Июнь 29, 2024

Abstract Background Wound management is a critical procedure in veterinary practice. A wound an injury that requires the body’s cells’ alignment to break down due external assault, such as trauma, burns, accidents, and diseases. Re-epithelization, extracellular matrix deposition, especially collagen, inflammatory cell infiltration, development of new blood capillaries are four features used evaluate healing process. Using natural extract for preferred avoid side effects synthetic drugs. The current study aimed assess effect major pregnane glycoside arabincoside B (AR-B) isolated from Caralluma arabica (C. arabica) Method AR-B was loaded on gel application. Rats were randomly distributed into six groups: normal, positive control (PC), MEBO®, 0.5%, 1%, 1.5%, be 6 animals each group. Wounds initiated under anesthesia with 1 cm diameter tissue needle, treatments applied daily 14 days. collected samples tested SOD, NO, MDA. Gene expression VEGF Caspase-3. Histopathological evaluation performed at two-time intervals (7 days), immunohistochemistry done α -SMA, TGF-β, TNF-α. Result It found treatment enhanced treated groups showed reduced MDA NO tissue, SOD activity increased. Re-epithelization deposition significantly improved, which confirmed by increase TGF-β -SMA well increased collagen deposition. TNF-α reduced, indicated subsiding inflammation. Caspase-3 reduced. Conclusion Our findings efficiency enhancing process its potential use topical dressing

Язык: Английский

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Anti-proliferative and Pro-apoptotic Activity of a Novel Oil-in-Water Nanoemulsion Containing Tea Tree Oil, Quercetin, and Rosmarinic Acid in Breast Cancer Cells

BioNanoScience, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 23, 2025

Язык: Английский

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Pseudo ternary phase diagram based PEGylated nano-dispersion of linezolid to promote wound regeneration: An in vitro and in vivo evaluation

Journal of drug targeting, Год журнала: 2025, Номер unknown, С. 1 - 15

Опубликована: Фев. 1, 2025

Open wounds are prone to bacterial infiltration mostly resistant strains like methicillin-resistant Staphylococcus aureus (MRSA), which affects healing of open wounds. Topical linezolid nano-dispersion using essential oils as nanoemulgel can increase solubility drug and bypass side-effects GI-irritation oral administration. Pseudo-ternary phase diagram was built optimise nanoemulsion. Surfactant/co-surfactant mixture (3:1), deionised water Oilmix (4:1) with were vortexed then ultrasonicated. 1% carbopol gel optimised nanoemulsion prepared characterised, exposed antimicrobial study, cytocompatibility study HEK293 cell-line, in vivo wound rat excision model. Histological performed confirm growth stratum corneum. Optimised formulation has particle size (244.6 ± 178.66 nm), polydispersity index (25%), entrapment efficiency (92.3 3.38%) vitro release (87.58 4.16%) best fitted Korsmeyer-Peppas kinetics Nanoemulgel F6 (0.2%w/w) found viscosity 5345 6 cP constituting a very excellent effect against MRSA. cells had shown good formulation. The contraction rate 99.66 0.57% at day 15 on daily application corneum almost fully regenerated. developed potential efficacy promote healing.

Язык: Английский

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