Quantitative Changes in the Proteome of Chronically Inflamed Lacrimal Glands From a Sjögren's Disease Animal Model
Investigative Ophthalmology & Visual Science,
Год журнала:
2025,
Номер
66(4), С. 44 - 44
Опубликована: Апрель 17, 2025
The
lacrimal
gland
(LG)
is
the
major
source
of
aqueous
tears,
and
insufficient
LG
secretion
leads
to
aqueous-deficient
dry
eye
(ADDE)
disease.
To
provide
a
foundational
description
LG's
protein
expression
patterns,
we
prepared
extracts
LGs
from
wild-type
an
ADDE
mouse
model
analyzed
proteome
by
quantitative
mass
spectrometry.
were
isolated
model,
male
non-obese
diabetic
(NOD)
mice
control
BALB/c
(n
=
6
each).
Protein
samples
in
urea-based
lysis
buffer
concentrations
determined
BCA
method.
equivalent
200
µg
tryptically
digested
nanoflow
liquid
chromatography
tandem
spectrometry
(LC-MS/MS).
Proteins
identified
quantified
using
PEAKS
X
bioinformatics
suite.
Downstream
differential
analysis
was
performed
MS-DAP
R
package.
Selected
significantly
differentially
expressed
detected
proteins
subjected
spatial
immunohistochemistry.
Cumulatively,
LC-MS/MS-based
proteomics
analyses
murine
total
31,932
peptide
sequences
resulting
2617
identifications
at
1%
false
discovery
rate
level.
Principal
component
(PCA)
hierarchical
cluster
revealed
separation
NOD
samples.
Overall,
diversity
consistently
higher
After
applying
global
filter
criteria
peptide-to-protein
rollup,
1750
remaining
MSqRob
algorithm,
which
580
with
statistically
significant
differences.
Data
are
available
via
ProteomeXchange
identifier
PXD060937.
At
cellular
level,
up-
downregulation
select
confirmed
Our
data
suggest
that
chronic
inflammation
alterations
proteome.
Ongoing
studies
aim
identify
potentially
unique,
inflammation-induced
could
be
amenable
pharmacological
modulation.
Язык: Английский
Evaluation of a fermented palm kernel meal as a prebiotic for enhancing immune response in Indonesian red claw crayfish (Cherax quadricarinatus)
Veterinary World,
Год журнала:
2025,
Номер
unknown, С. 896 - 906
Опубликована: Апрель 1, 2025
Background
and
Aim:
The
Indonesian
red
claw
crayfish
(Cherax
quadricarinatus)
represents
a
significant
aquaculture
commodity
with
substantial
economic
importance;
however,
disease
outbreaks,
particularly
tail
ulceration
caused
by
Aeromonas
hydrophila,
pose
challenges
to
sustainable
production.
This
study
evaluated
the
efficacy
of
fermented
palm
kernel
meal
(FPKM),
rich
in
mannan
oligosaccharides,
as
prebiotic
additive
enhance
non-specific
immune
responses
C.
quadricarinatus.
Materials
Methods:
A
total
120
(mean
weight
10.3
±
0.15
g
length
7.53
0.26
cm)
were
allocated
completely
randomized
design
comprising
four
dietary
treatments:
control
group
without
FPKM
probiotics
three
experimental
groups
receiving
diets
supplemented
at
concentrations
40,
80,
g/kg
along
probiotics.
Hemolymph
samples
collected
baseline
(day
0)
on
days
3,
5,
7,
14
post-treatment.
Non-specific
included
hemocyte
count
(THC),
differential
(DHC),
phagocytosis
activity
(PA),
index
(PI),
phenol
oxidase
(PO)
activity,
superoxide
dismutase
(SOD)
plasma
protein
(TPP),
gene
expression
(lipopolysaccharide
β-1,3-glucan-binding
[LGBP],
lectin).
Results:
inclusion
significantly
increased
THC,
peak
enhancement
observed
day
7
highest
concentration
(120
g/kg).
DHC
remained
stable
across
treatments.
Phagocytic
parameters,
including
PA
PI,
showed
improvements
(74.5
12.5%
2.8
0.41,
respectively)
treatment.
PO
SOD
activities
5
14,
respectively,
optimal
inclusion.
TPP
levels
did
not
exhibit
variation
among
Molecular
analyses
revealed
marked
upregulation
immune-related
genes,
notably
lectin
LGBP,
detected
40
group.
Conclusion:
Supplementing
substantially
improved
Optimal
was
generally
achieved
inclusion,
although
molecular
evident
lower
concentrations.
findings
underscore
potential
natural
sustainably
immunity,
thereby
reducing
dependency
antibiotics
contributing
eco-friendly
practices.
Keywords:
aquaculture,
crustacea,
systems,
meal,
prebiotic.
Язык: Английский
Research Advance About Plant Mannan Oligosaccharide Prebiotics from Fermented Palm Kernel Meal, Benefit for Boosting the Immune Response of Indonesian Red Claw Crayfish (Cherax Quadricarinatus)
Опубликована: Янв. 1, 2024
Язык: Английский
Transgenic overexpression of the miR‐200b/200a/429 cluster prevents mammary tumor initiation in Neu/Erbb2 transgenic mice
Katrina L. Watson,
Roger A. Moorehead
International Journal of Cancer,
Год журнала:
2024,
Номер
156(5), С. 993 - 1004
Опубликована: Окт. 6, 2024
Although
significant
progress
in
the
treatment
of
breast
cancer
has
been
achieved,
toxic
therapies
would
not
be
required
if
could
prevented
from
developing
first
place.
While
prevention
is
difficult
to
study
humans
due
long
disease
latency
and
stochastic
development,
transgenic
mouse
models
with
100%
incidence
defined
mammary
tumor
onset,
provide
excellent
for
studies.
In
this
study,
we
used
Neu/Erbb2
mice
(MTB-TAN)
as
a
model
human
HER2
Язык: Английский
Unveiling a unique macrophage population in exocrine glands sustained by ILC2-derived GM-CSF
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 3, 2024
Summary
Granulocyte-macrophage
colony-stimulating
factor
(GM-CSF)
has
a
non-redundant
role
in
the
emergence
and
maintenance
of
alveolar
macrophages
(AMs).
However,
its
developmental
steady-state
myelopoiesis
outside
lung
is
largely
unexplored.
Scanning
through
developing
tissues
using
Fate-map
reporter
GM-CSF
mouse
strain,
we
discovered
that
was
produced
by
type
2
innate
lymphoid
cells
(ILC2s)
submandibular
sublingual
salivary
gland
(SG)
during
postnatal
development.
producing
ILC2s
foster
development
hitherto
undescribed
phagocyte
subset,
which
named
adenophages.
Detailed
analysis
focusing
on
phenotypic
transcriptional
profiling
revealed
adenophages
display
shared
aspects
both,
dendritic
(DCs).
We
found
them
to
be
homogenously
distributed
across
SG,
but
always
close
proximity
myoepithelial
cells.
Importantly,
were
present
throughout
all
analyzed
exocrine
glands
such
as
lacrimal
mammary
glands,
also
identified
human
SG
sections,
indicating
conserved
species.
Язык: Английский
Tempol mitigates inflammation, oxidative stress, and histopathological alterations of cadmium-induced parotid gland injury in rats
Life Sciences,
Год журнала:
2024,
Номер
359, С. 123233 - 123233
Опубликована: Ноя. 8, 2024
Язык: Английский
Notch Signaling Drives Pro-Regenerative and Migratory Traits in Glandular Stem/Progenitor Cells
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 28, 2024
Abstract
Organoid
models
have
advanced
our
understanding
of
adult
stem/progenitor
cell
dynamics
and
function.
However,
uncovering
the
regulatory
mechanisms
scarce
often
quiescent
stem
cells
in
organs
like
salivary
glands
remains
challenging.
Using
single-cell
RNA
sequencing
bulk
ATAC
RNA-sequencing
analysis,
we
conducted
in-depth
profiling
cellular
populations
key
signaling
pathways
characterizing
a
mouse
submandibular
gland
organoid
(mSGO)
model
at
different
temporal
stages
response
to
radiation
damage.
We
identified
Sox9-
Itgb1-
expressing
as
most
primitive
uncover
novel
stemness
migratory
roles
for
Cd44
-expressing
cells.
Moreover,
revealed
that
Notch
is
essential
maintaining
self-renewal
migration
potential
these
post-irradiation.
Extending
findings
patient-derived
mSGOs,
well
murine
mammary
thyroid
organoids,
confirmed
conserved
regulator
function
under
migrative
regenerative
conditions.
Язык: Английский