Phytomedicine, Год журнала: 2024, Номер 135, С. 156242 - 156242
Опубликована: Ноя. 10, 2024
Язык: Английский
Nature Reviews Nephrology, Год журнала: 2024, Номер 20(10), С. 643 - 658
Опубликована: Май 9, 2024
Язык: Английский
Процитировано
17
Acta Physiologica, Год журнала: 2025, Номер 241(2)
Опубликована: Янв. 17, 2025
Members of the claudin protein family are major constituents tight junction strands and determine permeability properties paracellular pathway. In kidney, each nephron segment expresses a distinct subset claudins that form either barriers against solute transport or charge- size-selective channels. It was aim present study to compare permeation these renal ion channel-forming claudins. MDCK II cells, in which five had been knocked out (claudin quintupleKO), were stably transfected with individual mouse Cldn2, -4, -8, -10a, -10b, -15, dog Cldn16 -19, combination Cldn4 Cldn8, Cldn19. Permeation investigated Ussing chamber interactions by FRET assays. Claudin-4 -19 formed permeation. However, at low pH values absence HCO3 -, claudin-4 conveyed weak chloride nitrate permeability. Claudin-8 needed for assembly into TJ abolished this anion preference. Claudin-2, -16+19 highly permeable channels distinctive profiles different monovalent divalent anions cations, but ions opposite charge tracer fluorescein. Paracellular permeabilities along strictly determined expression patterns. specific certain thus lower transepithelial resistance, yet other solutes.
Язык: Английский
Процитировано
2
Cell Death and Disease, Год журнала: 2024, Номер 15(5)
Опубликована: Май 18, 2024
Abstract Lupus nephritis (LN) occurs in 50% of cases systemic lupus erythematosus (SLE) and is one the most serious complications that can occur during progression. Mesangial cells (MCs) are intrinsic kidney regulate capillary blood flow, phagocytose apoptotic cells, secrete vasoactive substances growth factors. Previous studies have shown various types inflammatory activate MCs for hyperproliferation, leading to disruption filtration barrier impairment renal function LN. Here, we characterized heterogeneity LN mice by single-nucleus RNA sequencing (snRNA-seq) revealed interaction between macrophages through CXC motif chemokine ligand 12 (CXCL12)/dipeptidyl peptidase 4 (DPP4) axis. In culture, modulated proliferation migration this ligand–receptor interaction. mice, treatment with linagliptin, a DPP4 inhibitor, effectively inhibited MC reduced urinary protein levels. Together, our findings indicated targeting CXCL12/DPP4 axis linagliptin may serve as novel strategy via
Язык: Английский
Процитировано
6
Frontiers in Bioscience-Landmark, Год журнала: 2024, Номер 29(7)
Опубликована: Июль 11, 2024
Podocytes are epithelial cells lining the outer surface of renal glomerular capillaries and they play a pivotal role in maintaining structural functional integrity filtration barrier. react to injury various ways any these highly specialized can progress podocyte dysfunction, resulting group proteinuric diseases called podocytopathies. Podocytopathies include wide spectrum primary secondary kidney diseases, including minimal change disease, diffuse mesangial sclerosis, focal segmental glomerulosclerosis, collapsing glomerulopathy, diabetic, membranous lupus nephropathies. Etiologically, be idiopathic, genetic or infections drugs, metabolic hemodynamic factors associated with immune non-immune systemic diseases. This manuscript provides basic understanding structure, causes injury, response subsequent progression The pathogenesis is set around podocytes. clinical morphological manifestations, commonality heterogeneity podocytopathies also discussed. As our knowledge biology improves, so will treatment avenues more podocyte-centric personalized approach.
Язык: Английский
Процитировано
4
Medicina Clínica, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Kidney International, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Novel transplantable organs need to be developed address the global organ shortage. Transplantation of embryonic kidney tissue, or metanephros, facilitates glomerular and tubular maturation offers partial functional support. However, adult environments do not permit exponential growth in size, limiting life-supporting functionality replacement effect this approach. Here, we a novel strategy that combines fusion bladders with multiple anastomoses host ureter, enabling significant increase metanephros transplantation urinary tract integration. By surgically anastomosing divided bladder segments, reconstructed excretory pathways by merging four metanephroi into each integrating them ureter. Following integration 20 at para-aortic region, anephric rats survived for over month generated approximately 50,000 nephrons vivo. Ultrastructural single-cell- transcriptomic analyses revealed maturity transplanted was comparable kidneys, although their small size likely contributed decreased urine concentration ability. Postoperative support helped normalize physiological homeostasis, including solute clearance, acid-base balance, electrolyte levels, hormone within vital ranges. Our findings underscore capacity dose-dependent therapeutic efficacy suggesting its potential as system.
Язык: Английский
Процитировано
0
BMC Nephrology, Год журнала: 2025, Номер 26(1)
Опубликована: Апрель 8, 2025
Язык: Английский
Процитировано
0
Medicina Clínica (English Edition), Год журнала: 2025, Номер 164(9), С. 470 - 479
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Май 15, 2025
Aging leads to functional decline across tissues, often accompanied by profound changes in cellular composition and cell-intrinsic molecular states. However, a comprehensive catalog of how the population individual cell types change with age associated epigenomic dynamics is lacking. Here, we constructed single-cell chromatin accessibility atlas consisting ∼7 million cells from 21 tissue spanning three groups both sexes. This dataset revealed 536 main 1,828 finer-grained subtypes, defined unique landscapes at ∼1.3 cis-regulatory elements. We observed widespread remodeling immune lineages, increases plasma macrophages, depletion T B progenitors. Additionally, non-immune populations, including kidney podocytes, ovary granulosa cells, muscle tenocytes lung aerocytes, showed marked reductions age. Meanwhile, many subtypes changed synchronously multiple organs, underscoring potential influence systemic inflammatory signals or hormonal cues. At level, aging was thousands differentially accessible regions, most concordant shared linked genes related inflammation development. Putative upstream factors, such as intrinsic shifts transcription factor usages extrinsic cytokine signatures, were identified. Notably, around 40% aging-associated sex-dependent differences, tens peaks altered exclusively one sex. Together, these findings present framework reshapes landscape diverse offering resource for understanding programs underlying supporting exploration targeted therapeutic strategies address age-related dysfunction.
Язык: Английский
Процитировано
0
Composites Part B Engineering, Год журнала: 2024, Номер 275, С. 111278 - 111278
Опубликована: Фев. 5, 2024
Kidney disease is a worldwide public health problem, seriously threatening the quality of life human beings, mainly including acute kidney injury (AKI), chronic (CKD), and end-stage renal (ESRD), with high mortality rate. Current effective treatments include dialysis transplants, but shortages donor organs, immune rejection, other problems have not been effectively addressed. In recent years, stem cells extensively studied, due to proliferation multi-directional differentiation properties, shown great potential for applications in field tissue regeneration, which may be an means repair regeneration. At same time, rapid development composite materials show excellent performance hemostasis, anti-inflammatory, organoids scaffolds construction, expected further guide promoting this review, we discuss latest research progress regeneration from perspectives cell extracellular vesicle (EVs) regulation, material combined used engineering kidneys culture. Besides, analyze summarize mechanisms as well pathways. addition, raise key issues that currently constrain composites therapy disease. Finally, possible future direction
Язык: Английский
Процитировано
2