Kidney development and regeneration: An introduction to this volume in Current Topics in Developmental Biology
Current topics in developmental biology/Current Topics in Developmental Biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Cell lineage tracing: Methods, applications, and challenges
Quantitative Biology,
Год журнала:
2025,
Номер
13(4)
Опубликована: Май 8, 2025
Abstract
Cell
lineage
tracing
is
a
crucial
technique
for
understanding
cell
fate
and
relationships,
with
wide
applications
in
developmental
biology,
tissue
regeneration,
disease
progression
studies.
Over
the
years,
experimental
methods
have
advanced
from
early
labeling
techniques
to
modern
genetic
tools
such
as
CRISPR‐Cas9‐based
barcoding,
whereas
computational
emerged
analyze
high‐dimensional
data
single‐cell
sequencing
other
omics
technologies.
This
paper
reviews
both
methods,
highlighting
their
respective
strengths,
limitations,
synergies.
Experimental
focus
on
tracking
cells,
approaches
reconstruct
relationships
model
cellular
dynamics.
Despite
significant
progress,
challenges
remain,
including
issues
accuracy,
resolution,
multi‐omics
integration,
scalability.
Future
directions
involve
improvements
development
of
enhanced
by
advancements
artificial
intelligence.
These
innovations
are
expected
drive
field
forward,
offering
potential
uncovering
mysteries
life.
Язык: Английский
A practical handbook on single-cell RNA sequencing data quality control and downstream analysis
Molecules and Cells,
Год журнала:
2024,
Номер
47(9), С. 100103 - 100103
Опубликована: Июль 31, 2024
Advancements
in
single-cell
analysis
have
facilitated
high-resolution
observation
of
the
transcriptome
individual
cells.
However,
standards
for
obtaining
high-quality
cells
and
data
pipelines
remain
variable.
Here,
we
provide
groundwork
improving
quality
by
delineating
guidelines
selecting
considerations
throughout
analysis.
This
review
will
streamline
researchers'
access
to
serve
as
a
valuable
guide
Язык: Английский