Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Март 10, 2024
Abstract
Globally,
breast
cancer
is
the
second
most
common
cause
of
cancer-related
deaths
among
women.
In
cancer,
microRNAs
(miRNAs)
are
essential
for
both
initiation
and
development
tumors.
It
has
been
suggested
that
tumor
suppressor
microRNA-561-3p
(miR-561-3p)
crucial
in
arresting
growth
cells.
Further
research
necessary
to
fully
understand
role
molecular
mechanism
miR-561
human
BC.
The
aim
this
study
was
investigate
inhibitory
effect
miR-561-3p
on
ZEB1
,
HIF1A
MYC
expression
as
oncogenes
have
impact
PD-L1
overexpression
cellular
processes
such
proliferation,
apoptosis,
cell
cycle
(BC)
lines.
genes
were
measured
BC
clinical
samples
lines
via
qRT-PCR.
luciferase
assay,
MTT,
Annexin-PI
staining,
experiments
used
assess
candidate
gene
expression,
progression.
Flow
cytometry
effects
suppression
line.
assay
showed
miRNA-561-3p
targets
3′-UTRs
significantly.
tissues,
qRT-PCR
results
demonstrated
downregulated
up-regulated.
shown
decreased
induced
apoptosis
arrest
through
downregulation
oncogenes.
Furthermore,
inhibition
by
reduced
at
mRNA
protein
levels.
Our
investigated
cells
first
time.
findings
may
help
clarify
regulation
point
miR
a
potential
biomarker
novel
therapeutic
target
immunotherapy.
Beilstein Journal of Nanotechnology,
Год журнала:
2023,
Номер
14, С. 912 - 926
Опубликована: Сен. 4, 2023
Nanotechnology
provides
effective
methods
for
precisely
delivering
chemotherapeutics
to
cancer
cells,
thereby
improving
efficacy
and
reducing
off-target
side
effects.
The
targeted
delivery
of
nanoscale
is
accomplished
by
two
different
approaches,
namely
the
exploitation
leaky
tumor
vasculature
(EPR
effect)
surface
modification
nanoparticles
(NPs)
with
various
tumor-homing
peptides,
aptamers,
oligonucleotides,
monoclonal
antibodies
(mAbs).
Because
higher
binding
affinity
specificity,
mAbs
have
received
a
lot
attention
detection
selective
biomarkers
also
treatment
types
cancer.
Antibody-conjugated
(ACNPs)
are
an
therapy
efficient
specifically
cells.
ACNPs
combine
benefits
NPs
provide
high
drug
loads
at
site
better
selectivity
efficiency.
on
NP
surfaces
recognize
their
specific
receptors
expressed
target
cells
release
chemotherapeutic
agent
in
controlled
manner.
Appropriately
designed
synthesized
essential
fully
realize
therapeutic
benefits.
In
blood
stream,
instantly
interact
biological
molecules,
protein
corona
formed.
Protein
formation
triggers
immune
response
affects
targeting
ability
nanoformulation.
this
review,
we
recent
findings
highlight
several
antibody
conjugation
such
as
adsorption,
covalent
conjugation,
biotin–avidin
interaction.
This
review
overview
many
effects
theranostic
applications
Cancers,
Год журнала:
2025,
Номер
17(2), С. 234 - 234
Опубликована: Янв. 13, 2025
The
complex
signaling
network
within
the
breast
tumor
microenvironment
is
crucial
for
its
growth,
metastasis,
angiogenesis,
therapy
escape,
stem
cell
maintenance,
and
immunomodulation.
An
array
of
secretory
factors
their
receptors
activate
downstream
cascades
regulating
cancer
progression
metastasis.
Among
various
pathways,
EGFR,
ER,
Notch,
Hedgehog
pathways
have
recently
been
identified
as
in
terms
proliferation,
survival,
differentiation,
maintenance
CSCs,
failure.
These
mediate
such
MAPK,
including
MEK/ERK
that
promote
common
pro-oncogenic
signaling,
whereas
dysregulation
PI3K/Akt,
Wnt/β-catenin,
JAK/STAT
activates
key
oncogenic
events
drug
resistance,
CSC
enrichment,
metabolic
reprogramming.
Additionally,
these
orchestrate
an
intricate
interplay
between
stromal
cells,
immune
cells.
Metabolic
reprogramming
adaptations
contribute
to
aggressive
are
unresponsive
therapy.
Herein,
recent
insights
into
novel
operating
TME
aid
advancement
emphasized
current
developments
practices
targeting
enhance
treatment
efficacy
reviewed.
Genes,
Год журнала:
2021,
Номер
12(8), С. 1206 - 1206
Опубликована: Авг. 4, 2021
The
programmed
death-ligand
1
(PD-L1)/programmed
cell
death
protein
(PD-1)
is
a
well-established
inhibitory
immune
checkpoint
axis
in
triple-negative
breast
cancer
(TNBC).
Growing
evidence
indicates
that
tumoral
PD-L1
can
lead
to
TNBC
development.
Although
conventional
inhibitors
have
improved
patients'
prognosis,
their
effect
mainly
focused
on
improving
anti-tumoral
responses
without
substantially
regulating
oncogenic
signaling
pathways
cells.
Moreover,
the
cannot
impede
de
novo
expression
of
oncoproteins,
like
PD-L1,
Accumulating
has
indicated
restoration
specific
microRNAs
(miRs)
downregulate
and
inhibit
Since
miRs
target
multiple
mRNAs,
miR-based
gene
therapy
be
an
appealing
approach
restore
responses,
regulate
various
intracellular
singling
TNBC.
Therefore,
we
conducted
current
systematic
review
based
preferred
reporting
items
for
reviews
meta-analyses
(PRISMA)
provide
comprehensive
unbiased
synthesis
currently
available
regarding
PD-L1-inhibiting
development
tumor
microenvironment.
For
this
purpose,
systematically
searched
Cochrane
Library,
Embase,
Scopus,
PubMed,
ProQuest,
Web
Science,
Ovid,
IranDoc
databases
obtain
relevant
peer-reviewed
studies
published
before
25
May
2021.
Based
evidence,
miR-424-5p,
miR-138-5p,
miR-570-3p,
miR-200c-3p,
miR-383-5p,
miR-34a-5p,
miR-3609,
miR-195-5p,
miR-497-5p
expression,
transform
immunosuppressive
microenvironment
into
pro-inflammatory
microenvironment,
proliferation,
suppress
migration,
enhance
chemosensitivity
cells,
stimulate
apoptosis,
arrest
cycle,
repress
clonogenicity
Concerning
biocompatibility
biomimetic
carriers
valuable
insights
provided
by
single-cell
sequencing
technologies,
sequencing-guided
delivery
these
decrease
toxicity
traditional
approaches,
increase
specificity
miR-delivery,
efficacy
miR
delivery,
affected
patients
with
personalized
therapy.
Bioengineered,
Год журнала:
2021,
Номер
12(1), С. 803 - 814
Опубликована: Янв. 1, 2021
The
present
study
aimed
to
investigate
the
role
and
underlying
mechanisms
of
long
non-coding
RNA
(lncRNA)
muscleblind-like
1
antisense
(MBNL1-AS1)
in
progression
Prostate
cancer
(PCa).
MBNL1-AS1
microRNA
(miR)-181a-5p
expression
PCa
tissues
several
human
cell
lines
were
analyzed,
respectively,
using
StarBasev3.0
project
RT-qPCR
assay.
After
overexpression,
proliferation,
invasion
migration
were,
evaluated
CCK-8,
colony
formation,
transwell
wound
healing
assays.
Dual
luciferase
assay
used
for
analysis
interactions
among
MBNL1-AS1,
miR-181a-5p,
phosphatase
tensin
homolog
(PTEN).
Subsequently,
PTEN
proteins
PI3K/AKT/mTOR
signaling
was
examined
western
blot
after
transfection
with
miR-181a-5p
mimic.
rescue
assays
performed
effects
on
functions
cells
PTEN/PI3K/AKT/mTOR
by
co-transfection
plasmid
Results
indicated
that
conspicuously
downregulated
while
upregulating
lines.
overexpression
decreased
abilities
invasion,
migration.
Further
revealed
acted
as
a
sponge
positively
regulated
effect.
Additionally,
proved
effect
MBNL1-AS1-upregulation
dependent
miR-181a-5p.
Furthermore,
counteracted
exerted
cells.
This
suggests
inhibits
via
sponging
regulating
pathway.
