Proteomic profiling of mesenchymal stem cell‐derived extracellular vesicles: Impact of isolation methods on protein cargo

Journal of Extracellular Biology, Год журнала: 2024, Номер 3(6)

Опубликована: Июнь 1, 2024

Abstract Extracellular vesicles (EVs) are nanosized with a lipid bilayer that secreted by cells and play critical role in cell‐to‐cell communication. Despite the promising reports regarding their diagnostic therapeutic potential, utilization of EVs clinical setting is limited due to insufficient information about cargo lack standardization isolation analysis methods. Considering protein cargos as key contributors potency, we conducted tandem mass tag (TMT) quantitative proteomics three subpopulations mesenchymal stem cell (MSC)‐derived obtained through different techniques: ultracentrifugation (UC), high‐speed centrifugation (HS), on sucrose cushion (SU). Subsequently, checked EV marker expression, size distribution, morphological characterization, followed bioinformatic analysis. The proteome results revealed these exhibit distinct molecular functional characteristics. choice method impacts isolated isolating EVs. Specifically, (HS) exhibited higher abundance ribosomal mitochondrial proteins. Functional apoptosis assays comparing mitochondria methods HS‐EVs, but not other EVs, induced early cancer cells. On hand, using (SU) (UC) demonstrated proteins primarily involved immune response, cell‐cell interactions extracellular matrix interactions. Our analyses unveil notable disparities associated biological functions among subpopulations, underscoring importance meticulously selecting resultant based intended application.

Язык: Английский

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Comparative effects of various extracellular vesicle subpopulations derived from clonal mesenchymal stromal cells on cultured fibroblasts in wound healing-related process

The International Journal of Biochemistry & Cell Biology, Год журнала: 2025, Номер unknown, С. 106737 - 106737

Опубликована: Янв. 1, 2025

Язык: Английский

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Immunomodulatory effects of mesenchymal stem cell therapy in chronic kidney disease: a literature review

BMC Nephrology, Год журнала: 2025, Номер 26(1)

Опубликована: Март 3, 2025

Chronic kidney disease (CKD) has been a growing public medical concern in recent years which calls for effective interventions. Mesenchymal stem cells (MSCs) have garnered increased interest past decades due to their potential repair and regenerate damaged tissues. Many clinical trials highlighted the safety effectiveness of with this novel cell therapy. MSC infusion can improve renal function indices such as glomerular filtration rate, urine protein, serum creatinine, blood urea nitrogen, while inhibiting immune response by increasing regulatory T cells. The therapeutic mechanisms may be primarily attributed combined immunomodulation, anti-inflammation, anti-fibrosis, promoting angiogenesis, anti-oxidation, anti-apoptosis, or tissue healing produced secretsome. However, CKD is broad concept many pathological etiologies including diabetes, hypertension, heart disease, immunological damage, family history failure, so on. Furthermore, efficacy MSCs influenced different sources, injection methods, medication dosage, homing proportion. As result, it timely essential access advancements application on CKD.

Язык: Английский

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Proteomic Profiling of Mesenchymal Stem Cell-Derived Extracellular Vesicles: Impact of Isolation Methods on Protein Cargo

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 7, 2024

Abstract Extracellular vesicles (EVs) are nanosized with a lipid bilayer that secreted by cells and play critical role in cell-to-cell communication. Despite the promising reports regarding their diagnostic therapeutic potential, utilization of EVs clinical setting is limited due to insufficient information about cargo lack standardization isolation analysis methods. Considering protein cargos as key contributors potency, we conducted tandem mass tag (TMT) quantitative proteomics three subpopulations mesenchymal stem cell (MSC)-derived obtained through different techniques: ultracentrifugation (UC), high-speed centrifugation (HS), on sucrose cushion (SU). Subsequently, checked EV marker expression, size distribution, morphological characterization, followed bioinformatic analysis. The proteome results revealed these exhibit distinct molecular functional characteristics. choice method impacts isolated isolating EVs. Specifically, (HS) exhibited higher abundance ribosomal mitochondrial proteins. Functional apoptosis assays comparing mitochondria methods HS-EVs, but not other EVs, induced early cancer cells. On hand, using (SU) (UC) demonstrated proteins primarily involved immune response, cellLJcell interactions, extracellular matrix interactions. Our analyses unveil notable disparities associated biological functions among subpopulations, underscoring importance meticulously selecting resultant based intended application.

Язык: Английский

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Olig2+ single-colony-derived cranial bone-marrow mesenchymal stem cells achieve improved regeneration in a cuprizone-induced demyelination mouse model

Journal of Zhejiang University SCIENCE B, Год журнала: 2024, Номер unknown

Опубликована: Сен. 25, 2024

Язык: Английский

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Allogeneic bone marrow derived clonal mesenchymal stromal cells in refractory rheumatoid arthritis: a pilot study

Regenerative Medicine, Год журнала: 2024, Номер 19(12), С. 599 - 609

Опубликована: Дек. 1, 2024

This phase I trial assessed the safety and potential efficacy of monthly 3 dose intravenous infusion allogeneic bone marrow-derived clonal mesenchymal stromal cells (BM-cMSCs) in refractory rheumatoid arthritis (RA) patients over 24 weeks.

Язык: Английский

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