Simultaneous detection of dengue virus serotypes in a dual-serotype-detection nucleic acid based lateral flow assay

Diagnostic Microbiology and Infectious Disease, Год журнала: 2025, Номер 111(3), С. 116679 - 116679

Опубликована: Янв. 6, 2025

Язык: Английский

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Long-term thermal stress induces hepatic injury and alters the thermotolerance response in Hong Kong catfish (Clarias fuscus)

Aquaculture, Год журнала: 2024, Номер 590, С. 741041 - 741041

Опубликована: Май 6, 2024

Global climate change has a significant impact on fish survival and the aquaculture industry. Even highly adaptable organisms like Siluriformes are susceptible to these effects. This study investigates consequences of extreme high temperature Hong Kong catfish (Clarias fuscus), species known for its long-term habitation in tropical subtropical regions. In this study, C. fuscus were cultivated 90 days at (HT, 34 °C) normal (NT, 26 °C), followed by subjecting two groups stress (34 recovery (26 °C). The hepatic histology, biochemical, transcriptomic characteristics examined before acute (NT-C, HT-C), after (NT-T, HT-T), (NT-R, HT-R). histological analysis revealed that heat damaged liver tissue fuscus. Furthermore, damage was observed tissues NT group under stress, while no further HT group. biochemical showed altered body's immune oxidative balance, resulting an increase alkaline phosphatase (ALP), alanine aminotransferase (ALT), catalase (CAT), superoxide dismutase (SOD) glutathione peroxidase (GPX) activities, decrease malondialdehyde (MDA) content. Upon function disordered unable produce enough total-bilirubin (T-BIL), aspartate (AST) activity more being affected. Long-term led changes gene expression, with showing three times differentially expressed genes (DEGs) identified compared conditions. Analysis DEG enrichment during same treatment period both differences amino acid metabolism lipid pathways. These results suggest caused fuscus, altering enzyme sensitivity, as well response pathways intensity. lays groundwork future investigations into adaptability sudden strategies mitigate facing temperatures.

Язык: Английский

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The endoplasmic reticulum (ER): a crucial cellular hub in flavivirus infection and potential target site for antiviral interventions

npj Viruses, Год журнала: 2024, Номер 2(1)

Опубликована: Июнь 21, 2024

Abstract Dengue virus (DENV) is the most prevalent arthropod-borne flavivirus and imposes a significant healthcare threat worldwide. At present no FDA-approved specific antiviral treatment available, safety of vaccine against DENV still on debate. Following its entry into host cell, takes advantage cellular secretory pathway to produce new infectious particles. The key organelle cell in infections endoplasmic reticulum (ER) which supports various stages throughout entire life cycle flaviviruses. This review delves intricate interplay between flaviviruses ER during their with focus molecular mechanisms underlying viral replication, protein processing virion assembly. Emphasizing significance cycle, we highlight potential targets ER-related steps replication summarize current drugs that are (pre)clinical developmental stage. Insights exploitation by offer promising avenues for development targeted strategies, providing foundation future research therapeutic interventions

Язык: Английский

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IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A

Cell Communication and Signaling, Год журнала: 2023, Номер 21(1)

Опубликована: Ноя. 9, 2023

Abstract The unfolded protein response is a survival signaling pathway that induced during various types of ER stress. Here, we determine IRE1’s role in miRNA regulation During induction stress human bronchial epithelial cells, utilized next generation sequencing to demonstrate pre-miR-301a and pre-miR-106b were significantly increased the presence an IRE1 inhibitor. Conversely, using nuclear-cytosolic fractionation on stressed found these pre-miRNAs decreased nuclear fractions without We also miR-301a-3p targets proapoptotic UPR factor growth arrest DNA-damage-inducible alpha ( GADD45A ). Inhibiting levels or blocking its predicted binding site ’s 3’ UTR with target protector mRNA expression. Furthermore, elevation XBP1s expression had no effect introduction for promoted cell death airway cells. In summary, results indicate endonuclease activity two-edged sword can splice XBP1 stabilize degrade elevate lead apoptosis.

Язык: Английский

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AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 6, 2024

Flaviviruses strategically utilize the endoplasmic reticulum (ER) in their replication cycles. However, role of ER autophagy (ER-phagy) viral process remains poorly understood. Here, we reveal that prolonged Zika virus (ZIKV) infection results from degradation ER-phagy receptor FAM134B, facilitated by NS2A protein. Mechanistically, ER-localized undergoes K48-linked polyubiquitination at lysine (K) 56 E3 ligase AMFR. Ubiquitinated binds to FAM134B and AMFR orchestrates NS2A-FAM134B complexes. AMFR-catalyzed ubiquitination not only targets but also hinders FAM134B-AMFR axis. Notably, a recombinant ZIKV mutant (ZIKV-NS2AK56R), lacking inhibition, exhibits attenuation ZIKV-induced microcephalic phenotypes human brain organoids replicates less efficiently, resulting weakened pathogenesis mouse models. In this work, our mechanistic insights propose flaviviruses manipulate modulate turnover, driving infection. Furthermore, AMFR-mediated flavivirus emerges as potential determinant pathogenecity. cycles, authors show key inhibit physiologically elucidate basic mechanisms pathogenicity regulating ER-phagy.

Язык: Английский

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Dengue Virus and Lipid Metabolism: Unraveling the Interplay for Future Therapeutic Approaches

Emerging Microbes & Infections, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

In recent years, Dengue virus (DENV) has continued to pose significant health risks in tropical and subtropical areas worldwide, raising alerts worldwide. It can cause hyperviremia humans even lead fatal clinical diseases. The life cycle of DENV is intricately linked cellular lipids, the selectively utilizes relevant enzymes involved lipid metabolism modulate existing metabolic system host cells during entry, replication, assembly, other stages, thereby creating an environment conducive its complete replication cycle. At present, there a lack effective specific anti-DENV treatment measures. This review summarizes recently identified molecules related diseases that affect infection, explores dependence on provides potential targets for dengue fever (DF).

Язык: Английский

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Advances in Nanobiosensors for Rapid and Sensitive Detection of Dengue Virus Biomarkers by Using Clinical Laboratory

Journal of Clinical Laboratory Analysis, Год журнала: 2025, Номер unknown

Опубликована: Март 13, 2025

ABSTRACT Background The recent rise in dengue virus (DENV) cases poses a significant threat to human health, with infections ranging from mild severe and potentially leading premature death. Objective To highlight the importance of early detection DENV review advancements technologies, particularly focusing on nanobiosensors. Methods This examines traditional methods for DENV, including molecular, serological, direct culture techniques, while discussing their limitations. It also explores innovative technologies that enhance accuracy, speed, efficiency. Results Nonstructural protein 1 (NS1) serves as key biomarker present high concentrations during stages infection, underscoring need timely detection. Traditional methods, effective, have limitations new aim address. Biosensors, nanobiosensors, emerged promising tools rapid, sensitive, cost‐effective Conclusion adoption advanced especially is crucial improving management reducing suffering. provides comprehensive overview nanobiosensors applications, presented an accessible manner readers.

Язык: Английский

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Endoplasmic reticulum stress triggers unfolded protein response as an antiviral strategy of teleost erythrocytes

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 26, 2024

Fish nucleated red blood cells (RBCs), also known as erythrocytes, play a crucial role in maintaining immune system balance by modulating protein expression response to various stimuli, including viral attack. This study explores the intriguing behavior of rainbow trout RBCs when faced with hemorrhagic septicemia virus (VHSV), focusing on endoplasmic reticulum (ER) stress and unfolded (UPR).

Язык: Английский

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Long-term thermal acclimation enhances heat resistance of Hong Kong catfish (Clarias fuscus) by modulating gill tissue structure, antioxidant capacity and immune metabolic pathways

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 284, С. 116930 - 116930

Опубликована: Авг. 27, 2024

The rapid temperature changes caused by global warming significantly challenge fish survival affecting various biological processes. Fish generally mitigate stress through physiological plasticity, but when exceed their tolerance limits, even adaptable species like Siluriformes can experience internal disruptions. This study investigates the effects of extreme thermal climate on Hong Kong catfish (Clarias fuscus), native to tropical and subtropical regions. C. fuscus were exposed normal (NT, 26 ℃) or high (HT, 34 condition for 90 days. Subsequently, histological, biochemical, transcriptomic in gill tissue observed after exposure acute temperatures (34 subsequent recovery (26 ℃). Histological analysis revealed that HT group exhibited less impact from sudden shifts compared NT group, as they adapted reducing interlamellar cell mass (ILCM) lamellae thickness (LT) tissue, thereby mitigating aftermath heat shock. Biochemical showed catalase (CAT) activity continued increase, while malondialdehyde (MDA) levels decreased, suggesting establishment a new oxidative balance enhanced environmental adaptability. Transcriptome identified 520 463 differentially expressed genes groups, respectively, response changes. Enrichment highlighted changes, inhibited apoptosis ferroptosis regulating alox12, gclc, hmox1a, attenuating adverse stress. Conversely, increased pfkma pkma provide sufficient energy repair. higher degree shock protein (Hsp) also indicated more severe injury. These findings demonstrate alterations structure, regulation balance, immune metabolic pathways fluctuations following exposure, potential avenues further exploration into plasticity adapting warming.

Язык: Английский

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Immunomodulation in dengue: towards deciphering dengue severity markers

Cell Communication and Signaling, Год журнала: 2024, Номер 22(1)

Опубликована: Сен. 26, 2024

Язык: Английский

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