Oyster Shell Powder/PCL Composite Scaffolds Loaded with Psoralen Nanospheres Promote Large Bone Defect Repair

ACS Omega, Год журнала: 2025, Номер 10(2), С. 2231 - 2242

Опубликована: Янв. 9, 2025

Research on bone substitutes for repairing defects has drawn increasing attention, and the efficacy of three-dimensional (3D) printed bioactive porous scaffolds defect repair been well documented. Our previous studies have shown that psoralen can promote osteogenesis by activating Wnt/β-catenin BMP/Smad signaling pathways their crosstalk effects, nanospheres a good osteogenesis-promoting effect in vitro with low cytotoxicity. The Chinese medicine oyster shell powder, characterized its structure, strong adsorption, unique bioactivity, potential fracture-promoting materials. However, loading onto powder/polycaprolactone (OSP/PCL) to is unclear. In this study, composite consisting OSP PCL were prepared 3D printing, adhered scaffolds. characterization features scaffold system investigated concerning biocompatibility mesenchymal stem cells (BMSCs). was explored vivo through rat cranial model. results showed homogeneous structures high mechanical strength could be psoralen-loaded nanospheres. had BMSCs positively affected expression osteogenic differentiation-related proteins. more effectively formation new area (Φ5 mm) compared pure At same time, containing significant stimulating healing OSP/PCL without psoralen.

Язык: Английский

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Autonomous living materials and bone-inspired scaffolds motivated by human osteogenic microenvironment mechanisms

Materials & Design, Год журнала: 2024, Номер 244, С. 113145 - 113145

Опубликована: Июль 8, 2024

The repair of secondary critical bone defects is an international medical challenge. Bone tissue engineering provides methods and technology for repair. regeneration mechanism serves as inspiration the material structural design scaffolds. In terms materials, this review draws from biological characteristics host cells in osteogenic microenvironment (including osteoblast lineage, vascular cell inflammatory cells, etc.), reviewing regulatory mechanisms self-healing proposing autonomous living materials scaffolds which prepared by in-situ manufacturing. Autonomous regulate migration, proliferation differentiation real time releasing steadily long-term. Regarding structure, we functional role natural structures homeostasis, providing insights into bone-inspired Due to conflict between mechanical properties ability, proposes assembled They can prolong half-life provide support attachment points new growth, autonomously microenvironment. have potential advance research progress field pave way novel clinical treatments.

Язык: Английский

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Psychological stress disturbs bone metabolism via miR-335-3p/Fos signaling in osteoclast

eLife, Год журнала: 2025, Номер 13

Опубликована: Янв. 8, 2025

It has been well validated that chronic psychological stress leads to bone loss, but the underlying mechanism remains unclarified. In this study, we established and analyzed unpredictable mild (CUMS) mice investigate miRNA-related pathogenic involved in stress-induced osteoporosis. Our result found these CUMS exhibited osteoporosis phenotype is mainly attributed abnormal activities of osteoclasts. Subsequently, miRNA sequencing other analysis showed miR-335-3p, which normally highly expressed brain, was significantly downregulated nucleus ambiguous, serum, mice. Additionally, vitro studies detected miR-335-3p important for osteoclast differentiation, with its direct targeting site Fos . Further demonstrated FOS upregulated osteoclast, inhibition suppressed accelerated osteoclastic as expression genes, such Nfatc1, Acp5, Mmp9 , miR-335-3p-restrained conclusion, work indicated may downregulate expression, resulted accumulation upregulation NFACT1 signaling pathway osteoclasts, leading differentiation activity. These results decipher a previously unrecognized paradigm can act link between metabolism.

Язык: Английский

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A novel strategy for bone defect repair: Stromal cell-derived factor 1α sustained-release acellular fish scale scaffolds combined with injection of bone marrow mesenchymal stem cells promote bone regeneration

Materials Today Bio, Год журнала: 2025, Номер unknown, С. 101759 - 101759

Опубликована: Апрель 1, 2025

Patients with bone defects often have weak cell vitality and differentiation ability of endogenous marrow mesenchymal stem cells (BMSCs), which makes regeneration face challenges. At present, the tissue engineering strategies are mainly to build grafts by loading on scaffolds in vitro. These many difficulties that limit their clinical application. To this end, we developed a new strategy for defect repair, namely chemotactic cell-free combined BMSCs injection. We first prepared polydopamine-functionalized acellular fish scale scaffold can continuously release stromal cell-derived factor 1α (SDF-1α) (termed as SDF-1α/PAFS) vivo at least 10 days. The study results showed not only has excellent mechanical properties good biocompatibility but also reactive oxygen scavenging activity, immunomodulation, angiogenesis, osteogenesis. More importantly, SDF-1α/PAFS recruit postoperatively injected into repair. constructed mouse cranial model, experimental confirmed combining injection effectively promote Overall, provides promising better convenience operability.

Язык: Английский

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Diabetes Mellitus Impairs the Bone Regeneration Capacity of Mesenchymal Stromal Cell-Based Therapy

Archives of Medical Research, Год журнала: 2025, Номер 56(6), С. 103234 - 103234

Опубликована: Май 20, 2025

Язык: Английский

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Psychological stress disturbs bone metabolism via miR-335-3p/Fos signaling in osteoclast

eLife, Год журнала: 2024, Номер 13

Опубликована: Апрель 29, 2024

It has been well validated that chronic psychological stress leads to bone loss, but the underlying mechanism remains unclarified. In this study, we established and analyzed unpredictable mild (CUMS) mice investigate miRNA-related pathogenic involved in stress-induced osteoporosis. Our result found these CUMS exhibited osteoporosis phenotype is mainly attributed abnormal activities of osteoclasts. Subsequently, miRNA sequencing other analysis showed miR-335-3p, which normally highly expressed brain, was significantly downregulated nucleus ambiguous, serum, mice. Additionally, vitro studies detected miR-335-3p important for osteoclast differentiation, with its direct targeting site Fos . Further demonstrated FOS upregulated osteoclast, inhibition suppressed accelerated osteoclastic as expression genes, such Nfatc1, Acp5, Mmp9 , miR-335-3p-restrained conclusion, work indicated may downregulate expression, resulted accumulation upregulation NFACT1 signaling pathway osteoclasts, leading differentiation activity. These results decipher a previously unrecognized paradigm can act link between metabolism.

Язык: Английский

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Efficacy of mesenchymal stem cell‐based therapy on the bone repair of hypertensive rats

Oral Diseases, Год журнала: 2024, Номер 30(8), С. 5118 - 5128

Опубликована: Май 19, 2024

Abstract Objective Hypertension disrupts the bone integrity and its repair ability. This study explores efficiency of a therapy based on application mesenchymal stem cells (MSCs) to defects spontaneously hypertensive rats (SHR). Methods First, we evaluated SHR in terms morphometry differentiation MSCs into osteoblasts. Then, effects interactions between from normotensive (NTR‐MSCs) cocultured with (SHR‐MSCs) osteoblast both cell populations were evaluated. Also, formation calvarial treated NTR‐MSCs was analyzed. Results induced loss evidenced by reduced morphometric parameters femurs compared NTR as well decreased SHR‐MSCs NTR‐MSCs. partially restored capacity differentiate osteoblasts, while exhibited slight negative effect An enhanced observed control, stressing this efficacy even bones damaged hypertension. Conclusion The use derived heathy environment can be near future smart approach treat context regenerative dentistry for oral rehabilitation patients.

Язык: Английский

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Psychological stress disturbs bone metabolism via miR-335-3p/Fos signaling in osteoclast

Опубликована: Дек. 19, 2024

It has been well-validated that chronic psychological stress leads to bone loss, but the underlying mechanism remains unclarified. In this study, we established and analyzed unpredictable mild (CUMS) mice investigate miRNA-related pathogenic involved in induced osteoporosis. Our result found these CUMS exhibited osteoporosis phenotype mainly attributed abnormal activities of osteoclasts. Subsequently, miRNA sequencing other analysis showed miR-335-3p, which is normally highly expressed brain, was significantly down-regulated nucleus ambiguous (NAC), serum, mice. Additionally, vitro studies detected miR-335-3p important for osteoclast differentiation, with its direct targeting site FOS. Further demonstrated FOS upregulated osteoclast, inhibition suppressed accelerated osteoclastic as well expression genes, such Nfatc1, Acp5, Mmp9 , restrained conclusion, work indicated may down-regulate expression, resulted accumulation up-regulation NFACT1 signaling pathway osteoclasts, leading differentiation activity. These results decipher a previously unrecognized paradigm can act link between metabolism.

Язык: Английский

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Psychological stress disturbs bone metabolism via miR-335-3p/Fos signaling in osteoclast

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 23, 2024

Abstract It has been well-validated that chronic psychological stress leads to bone loss, but the underlying mechanism remains unclarified. In this study, we established and analyzed unpredictable mild (CUMS) mice investigate miRNA-related pathogenic involved in induced osteoporosis. Our result found these CUMS exhibited osteoporosis phenotype mainly attributed abnormal activities of osteoclasts. Subsequently, miRNA sequencing other analysis showed miR-335-3p, which is normally highly expressed brain, was significantly down-regulated nucleus ambiguous (NAC), serum, mice. Additionally, vitro studies detected miR-335-3p important for osteoclast differentiation, with its direct targeting site FOS. Further demonstrated FOS upregulated osteoclast, inhibition suppressed accelerated osteoclastic as well expression genes, such Nfatc1, Acp5, Mmp9 , restrained conclusion, work indicated may down-regulate expression, resulted accumulation up-regulation NFACT1 signaling pathway osteoclasts, leading differentiation activity. These results decipher a previously unrecognized paradigm can act link between metabolism. Impact statement targets inhibits activation NFATC1 signaling, an regulator function responsible

Язык: Английский

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Diabetes mellitus impairs the bone regeneration capacity of mesenchymal stem cell-based therapy

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 27, 2024

BACKGROUND Diabetes mellitus (DM) negatively impacts bone tissue, leading to loss and increased fracture risk with many in need of additional treatments, therapy based on mesenchymal stem cells (MSCs) represents a promising treatment for defects patients diabetes. The present investigation explored the interactions between MSCs from normoglycemic (NG-MSCs) diabetic (DM-MSCs) donors osteoblast differentiation effects NG-MSCs regeneration created rats. METHODS After DM induction streptozotocin, we evaluated morphometric parameters femurs MSC differentiation, as well DM-MSCs differentiation. efficacy cell was measured by evaluating calvarial rats treated local injection either or vehicle. RESULTS induced impaired MSCs, which partially restored NG-MSCs, formation observed vehicle similar. CONCLUSION These findings indicate that beneficial effect did not translate enhanced repair, mostly due hostile environment hyperglycemia, compromises capacity induce formation.

Язык: Английский

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