Erythropoietin decreases apoptosis and promotes Schwann cell repair and phagocytosis following nerve crush injury in mice DOI Creative Commons
Prem Kumar Govindappa, Govindraj Ellur, John P. Hegarty

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 23, 2025

After peripheral nerve trauma, insufficient clearance of phagocytic debris significantly hinders regeneration. Without sufficient myelin clearance, Schwann cells (SCs) undergo increased apoptosis, impairing functional recovery. There is no treatment for crush injury (PNCI). Erythropoietin (EPO) an FDA-approved drug anemia, which may help in the PNCI by transdifferentiating resident SCs into repair (rSCs) and enhancing phagocytosis to facilitate removal cellular debris. For first time, we conducted bulk RNA sequencing on mice with calibrated sciatic injuries (SNCIs) days 3, 5, 7 post-SNCI uncover transcriptomic changes without EPO treatment. We found altered several biological pathways associated genes, particularly those involved cell differentiation, proliferation, phagocytosis, myelination, neurogenesis. validated effects SNCI early (days 3/5) intermediate (day 7) post-SNCI, reduced apoptosis (TUNEL), enhanced SC (c-Jun p75-NTR), proliferation (Ki67), rSCs at sites. This improvement corresponded index (SFI). also confirmed these findings in-vitro . during de-differentiation, marked high c-Jun p75-NTR protein levels, later re-differentiation EGR2 low levels. These occurred under lipopolysaccharide (LPS) stress 24 72h, respectively, compared LPS alone. Under stress, or dead SCs. In conclusion, our support enhance function as a basis its possible use treating trauma.

Язык: Английский

Erythropoietin decreases apoptosis and promotes Schwann cell repair and phagocytosis following nerve crush injury in mice DOI Creative Commons
Prem Kumar Govindappa, Govindraj Ellur, John P. Hegarty

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 23, 2025

After peripheral nerve trauma, insufficient clearance of phagocytic debris significantly hinders regeneration. Without sufficient myelin clearance, Schwann cells (SCs) undergo increased apoptosis, impairing functional recovery. There is no treatment for crush injury (PNCI). Erythropoietin (EPO) an FDA-approved drug anemia, which may help in the PNCI by transdifferentiating resident SCs into repair (rSCs) and enhancing phagocytosis to facilitate removal cellular debris. For first time, we conducted bulk RNA sequencing on mice with calibrated sciatic injuries (SNCIs) days 3, 5, 7 post-SNCI uncover transcriptomic changes without EPO treatment. We found altered several biological pathways associated genes, particularly those involved cell differentiation, proliferation, phagocytosis, myelination, neurogenesis. validated effects SNCI early (days 3/5) intermediate (day 7) post-SNCI, reduced apoptosis (TUNEL), enhanced SC (c-Jun p75-NTR), proliferation (Ki67), rSCs at sites. This improvement corresponded index (SFI). also confirmed these findings in-vitro . during de-differentiation, marked high c-Jun p75-NTR protein levels, later re-differentiation EGR2 low levels. These occurred under lipopolysaccharide (LPS) stress 24 72h, respectively, compared LPS alone. Under stress, or dead SCs. In conclusion, our support enhance function as a basis its possible use treating trauma.

Язык: Английский

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