Spheroid‐on‐a‐Chip Platforms for Tumor Microenvironment and Drug Development

Advanced Materials Technologies, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

Abstract Despite significant advancements in oncology research and therapeutic interventions, cancer continues to be the leading cause of mortality worldwide. The key challenge addressing this pressing issue lies lack precision diagnosis a limited understanding nature how tumor microenvironment responds interventions. Research focusing on impact (TME) heterogeneity response drugs is crucial ensure efficient therapy. Conventional models exhibit constraints, including inability accurately imitate tumors’ complex 3D architecture dynamic microenvironment. Recent developments Spheroid‐On‐a‐Chip (SoC) technology provide alternative, offering closer that human tissue. This review explores recent SoC modeling, highlighting spheroid formation mechanisms techniques. applications platform mimic essential features their potential role anticancer drug development are summarized. advantages challenges technologies behind devices compared traditional vitro further discussed. Lastly, future directions for transforming improving suggested.

Язык: Английский

A Benzodiazepine-Derived Molecule That Interferes with the Bio-Mechanical Properties of Glioblastoma-Astrocytoma Cells Altering Their Proliferation and Migration

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2767 - 2767

Опубликована: Март 19, 2025

Glioblastoma multiforme (grade IV glioma) is characterized by a high invasive potential, making surgical intervention extremely challenging and patient survival very limited. Current pharmacological approaches show, at best, slight improvements in the therapy against this type of tumor. Microtubules are often target antitumoral drugs, specific drugs affecting their dynamics acting on microtubule-associated proteins (MAPs) without producing depolymerization could affect both glioma cell migration/invasion proliferation. Here, we analyzed cellular model glioblastoma multiforme, effect molecule (1-(4-amino-3,5-dimethylphenyl)-3,5-dihydro-7,8-ethylenedioxy-4h2,3-benzodiazepin-4-one, hereafter named 1g) which was shown to act as cytostatic drug other types microtubule dynamics. We found that acts also migration suppressor inducing loss polarity. mechanics U87MG aggregates exposed 1g different biophysical techniques. considered 3D 2D cultures, testing substrates stiffness. established produces decrease spheroid contractility it impairs invasion. At same time, case isolated cells, selectively an almost instantaneous polarity blocking disorganization mitotic spindle when cells reach mitosis, leading frequent slippage events followed death. can state studied similar effects molecules known microtubules, but probably indirectly via following biochemical pathways. Consistently, report evidence that, regarding its morphology, shows specificity for some such cells. Interestingly, being derived from benzodiazepine, chemical structure allow easily cross blood–brain barrier. Thanks chemical/physical properties, be promising new treatment GBM.

Язык: Английский

Evaluation of the Cancer-Preventive Effect of Resveratrol-Loaded Nanoparticles on the Formation and Growth of In Vitro Lung Tumor Spheroids

Pharmaceutics, Год журнала: 2024, Номер 16(12), С. 1588 - 1588

Опубликована: Дек. 12, 2024

Background: Resveratrol (RSV) is a natural polyphenol that offers antioxidant, anti-inflammatory, and chemopreventive benefits. This project determined the ability of RSV-loaded nanoparticles (NP) to inhibit growth lung tumor spheroids in vitro. Methods: RSV was encapsulated NP comprised biodegradable polymer, acetalated dextran. A549 cancer cells two-dimensional three-dimensional cell culture models were exposed free evaluate their effect on proliferation spheroid formation growth. For prevention studies, day 0, for treatment dosed with same formulations 5 after had fully formed. Results: The resulting 200 nm diameter neutral surface charge exhibited control release vitro based environmental pH. In comparison RSV, exerted greater inhibitory spheroids. Conclusions: have potential be used as agent cancer.

Язык: Английский