Tracking the Evolution of Cutaneous Melanoma by Multiparameter Flow Sorting and Genomic Profiling DOI Open Access
Luca Roma, Thomas Lorber,

Sabrina Rau

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1758 - 1758

Опубликована: Фев. 19, 2025

Intratumoral heterogeneity and clonal evolution are pivotal in the progression metastasis of melanoma. However, when combined with variable tumor cellularity, intratumoral limits sensitivity accuracy uncovering a cancer's evolution. In this study, we fluorescence-activated cell sorting (FACS) whole-exome sequencing (WES) to investigate composition evolutionary patterns seven melanoma biopsies obtained from three patients, each having both primary site metastatic samples. We employed multiparameter ploidy approach isolate populations based on DNA biomarkers (SOX10 or S100), enabling us high resolution. Our increased mean purity 70% (range 19-88%) unsorted material 91% 87-96%) post-sorting. findings revealed significant inter- intratumor heterogeneity, one patient exhibiting two genomically distinct within single biopsy, giving rise different metastases. highlight critical role melanoma, especially analyzing trajectories. The unique combination FACS WES provides powerful method for identifying reconstructing This study valuable insights into architecture lays groundwork future research larger groups.

Язык: Английский

Tracking the Evolution of Cutaneous Melanoma by Multiparameter Flow Sorting and Genomic Profiling DOI Open Access
Luca Roma, Thomas Lorber,

Sabrina Rau

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1758 - 1758

Опубликована: Фев. 19, 2025

Intratumoral heterogeneity and clonal evolution are pivotal in the progression metastasis of melanoma. However, when combined with variable tumor cellularity, intratumoral limits sensitivity accuracy uncovering a cancer's evolution. In this study, we fluorescence-activated cell sorting (FACS) whole-exome sequencing (WES) to investigate composition evolutionary patterns seven melanoma biopsies obtained from three patients, each having both primary site metastatic samples. We employed multiparameter ploidy approach isolate populations based on DNA biomarkers (SOX10 or S100), enabling us high resolution. Our increased mean purity 70% (range 19-88%) unsorted material 91% 87-96%) post-sorting. findings revealed significant inter- intratumor heterogeneity, one patient exhibiting two genomically distinct within single biopsy, giving rise different metastases. highlight critical role melanoma, especially analyzing trajectories. The unique combination FACS WES provides powerful method for identifying reconstructing This study valuable insights into architecture lays groundwork future research larger groups.

Язык: Английский

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