Bridging the gap: Phage manufacturing processes from laboratory to agri-food industry
Virus Research,
Год журнала:
2025,
Номер
353, С. 199537 - 199537
Опубликована: Янв. 31, 2025
Язык: Английский
Isolation and Characterization of a Broad-Spectrum bacteriophage Against Multi-drug Resistant Escherichia coli from waterfowl field
Shaqiu Zhang,
Qiang Ye,
Mingshu Wang
и другие.
Poultry Science,
Год журнала:
2025,
Номер
104(2), С. 104787 - 104787
Опубликована: Янв. 10, 2025
Escherichia
coli
(E.
coli)
is
a
significant
pathogen
responsible
for
intestinal
infections
and
foodborne
diseases.
The
rise
of
antibiotic
resistance
poses
challenge
to
global
public
health.
Traditional
therapy
becoming
increasingly
ineffective,
highlighting
the
urgent
need
innovative
control
strategies.
This
study
explores
potential
bacteriophages
as
sustainable
alternative
traditional
antibiotics.
From
2021
2022,
total
183
non-repetitive
duck
source
fecal
samples
were
collected
from
Mianyang
City,
Sichuan
Province,
126
strains
E.
isolated.
minimum
inhibitory
concentration
(MIC)
test
showed
that
these
exhibited
high
piperacillin
(96.8%),
tetracycline
(88.9%),
chloramphenicol
(86.5%).
It
concerning
93.7%
isolates
are
classified
multidrug-resistant
(MDR),
posing
threat
existing
treatment
options.
20
isolated
soil
samples,
among
which
5
selected
further
analysis.
Bacteriophage
YP6
excellent
lytic
effects
on
MDR
strains,
especially
strain
MY104,
well
representative
serotypes
O1
MY51)
O18
MY106).
identification
member
Myoviridae
family
was
conducted
using
transmission
electron
microscopy,
it
found
have
an
optimal
infection
factor
0.1.
Bacteriophages
exhibit
thermal
pH
stability,
maintaining
survival
at
temperatures
up
60
°C
ranges
4
10.
Whole
genome
sequencing
confirmed
has
double
stranded
DNA
139,323
base
pairs
(bp),
no
or
virulence
genes
found,
indicating
low
possibility
horizontal
gene
transfer.
In
addition,
effectively
inhibits
formation
biofilm,
key
in
chronic
infections.
vivo
experiments
Galleria
mellonella
(G.
mellonella)
larvae
shown
protective
effect
against
infection.
summary,
bacteriophage
expected
become
therapeutic
agent
due
its
broad
host
range,
environmental
biofilm
inhibition
properties.
Future
research
should
optimize
preparations,
evaluate
safety
efficacy
animal
models,
establish
clinical
application
plans
field
food
safety.
Язык: Английский
Prevalence, trends, and molecular insights into colistin resistance among gram-negative bacteria in Egypt: a systematic review and meta-analysis
Annals of Clinical Microbiology and Antimicrobials,
Год журнала:
2025,
Номер
24(1)
Опубликована: Май 10, 2025
This
study
examines
colistin
resistance
in
Gram-negative
bacteria
Egypt,
analyzing
prevalence,
trends,
geographic
variations,
colistin-carbapenem
correlation,
and
mcr-mediated
plasmid
resistance.
We
conducted
a
systematic
search
of
articles
published
between
2014
2024
that
reported
on
or
isolated
from
human
infections
with
clearly
defined
susceptibility
testing
methods.
A
random-effects
meta-analysis
was
to
estimate
prevalence
based
broth
microdilution
(BMD)
findings,
the
gold
standard
method.
To
explore
influence
study-level
factors-including
alternative
methods-a
multivariate
meta-regression
analysis
performed.
The
results
are
as
regression
coefficients
(β),
representing
difference
resistance,
expressed
percentage
points.
All
statistical
analyses
were
using
R
software.
included
55
studies.
Based
BMD
testing,
observed
9%
all
recovered
isolates
(95%
CI:
6-14%)
significantly
higher
among
carbapenem-resistant
(31%,
95%
25-38%),
p
<
0.001.
Multivariate
further
confirmed
compared
total
(β
=
9.8%
points,
0.001).
Additionally,
has
increased
over
time,
β
1.8%
points
per
year
(p
use
VITEK
2
system
associated
lower
detected
-7.0,
0.02).
Geographically,
rates
Upper
Egypt
9.3,
0.04).
Regarding
mcr
plasmid-mediated
mcr-1
most
prevalent
gene,
particularly
E.
coli.
In
contrast,
mcr-2
rare,
sporadically
K.
pneumoniae
P.
aeruginosa.
identified
bacteria,
increasing
31%
isolates.
strains
suggests
stronger
selective
pressure
frequent
use.
shown
rising
trend
likely
driven
by
usage
spread
These
findings
underscore
urgent
need
for
strict
antimicrobial
stewardship
therapies
curb
evolution.
Язык: Английский
Exploring the Efficacy of Bacteriophage Cocktails for Mitigating Vibrio Contamination within the Seafood Production Chain: A Feasible Approach to Microbial Risk Management
Food Control,
Год журнала:
2025,
Номер
unknown, С. 111424 - 111424
Опубликована: Май 1, 2025
Язык: Английский
Bacteriophage cocktail LEC2-LEC10 for broad-spectrum control of pathogenic and uncharacterized Escherichia coli in fresh produce
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Май 19, 2025
Escherichia
coli
is
a
major
foodborne
pathogen
that
causes
intestinal
diseases
leading
to
severe
illness.
In
particular,
E.
contamination
in
fresh
produce
presents
significant
risk,
because
there
are
no
additional
sterilization
processes
before
consumption.
this
study,
we
characterized
two
novel
bacteriophages,
vB_EcoS_LEC2
and
vB_EcoS_LEC10,
explored
their
use
as
phage
cocktail
control
naturally
occurring
foods.
Two
phages
were
isolated,
antimicrobial
activity
target
bacterial
spectrum
analyzed.
The
efficacy
of
two-phage
was
evaluated
against
O157:H7
strain
mixtures
occurring,
unidentified
present
on
commercially
available
vegetables.
receptors
recognized
by
the
identified
using
receptor-deficient
mutants.
genome
sequences
compared,
focusing
receptor-binding
protein
genes.
Characterizations
revealed
both
belonged
Straboviridae
family
stable
under
various
temperatures
pH
conditions.
confirmed
be
strictly
lytic,
exhibiting
short
latent
periods
15
10
min
burst
sizes
22
189
particles
per
infected
cell
for
LEC2
LEC10,
respectively.
LEC10
exhibited
distinct
spectra,
each
with
broad
but
complementary
host
range
among
strains.
Combining
into
leveraged
specificities,
broadening
overall
enhancing
lysis
pathogenic
compared
individual
phages.
remarkably
reduced
viability
contaminated,
vegetables,
demonstrating
its
effectiveness
targeting
diverse
populations.
recognize
different
receptors,
specifically
lipopolysaccharide
(LPS)
(via
WaaC)
OmpC,
respectively,
supporting
compatibility
optimization.
Furthermore,
analysis
absence
lysogeny-related
genes,
toxins,
antibiotic
resistance
reinforcing
suitability
safe
biocontrol
agents
food
applications.
These
results
demonstrate
especially
when
used
cocktail,
promising
antibacterial
controlling
Their
ranges
strong
lytic
support
application
safety
strategies
aimed
at
reducing
contamination.
Язык: Английский
Current Knowledge on CRISPR Strategies Against Antimicrobial-Resistant Bacteria
Antibiotics,
Год журнала:
2024,
Номер
13(12), С. 1141 - 1141
Опубликована: Ноя. 27, 2024
CRISPR/Cas
systems
have
emerged
as
valuable
tools
to
approach
the
problem
of
antimicrobial
resistance
by
either
sensitizing
or
lysing
resistant
bacteria
aiding
in
antibiotic
development,
with
successful
applications
across
diverse
organisms,
including
and
fungi.
can
target
plasmids
bacterial
chromosome
AMR-bacteria,
it
is
especially
necessary
an
efficient
entry
into
cells,
which
be
achieved
through
nanoparticles
bacteriophages.
Regarding
development
production,
though
use
this
field
still
modest,
there
untapped
reservoir
fungal
natural
products,
over
95%
yet
characterized.
In
Streptomyces,
a
key
antibiotic-producing
genus,
has
been
successfully
used
activate
silent
biosynthetic
gene
clusters,
leading
discovery
new
antibiotics.
also
applicable
non-model
different
species
fungi,
making
versatile
tool
for
products
discovery.
Moreover,
CRISPR/Cas-based
studies
offer
insights
metabolic
regulation
pathways
both
highlighting
its
utility
understanding
genetic
improving
industrial
strains.
work,
we
review
ongoing
innovations
on
ways
treat
resistances
using
platforms,
role
fungi
these
processes.
Язык: Английский