Microorganisms,
Год журнала:
2025,
Номер
13(1), С. 160 - 160
Опубликована: Янв. 14, 2025
Lactobacillus
is
a
key
genus
of
probiotics
commonly
utilized
for
the
treatment
oral
infections
The
primary
aim
our
research
was
to
investigate
probiotic
potential
newly
isolated
Levilactobacillus
brevis
DPL5
strain
from
human
breast
milk,
focusing
on
its
ability
combat
biofilm-forming
pathogens
such
as
Staphylococcus
aureus.
Employing
in
vitro
approaches,
we
demonstrate
L.
DPL5′s
endure
at
pH
3
with
survival
rates
above
30%,
and
withstand
osmotic
stress
often
found
during
industrial
processes
like
fermentation
freeze
drying,
retaining
over
90%
viability.
lyophilized
cell-free
supernatant
had
significant
antagonistic
effect
against
biofilm-producing
nasal
strains
aureus,
it
completely
eradicated
biofilms
subinhibitory
concentrations
20
mg·mL−1.
Higher
69
mg·mL−1
were
have
99%
bactericidal
effect,
based
conducted
probability
analysis,
indicating
production
bioactive
extracellular
compounds
capable
disrupting
biofilm
formation
S.
Furthermore,
genome-wide
sequencing
analysis
cutting-edge
Nanopore
technology
has
uncovered
50
genes
linked
activity,
supporting
adapt
thrive
harsh
gut
environment.
genome
also
contains
multiple
biosynthetic
gene
clusters
lanthipeptide
class
IV,
Type
III
polyketide
synthase
(T3PKS),
ribosomally
synthesized,
post-translationally
modified
peptides
(RiPP-like
compounds),
all
which
are
associated
antibacterial
properties.
Our
study
paves
way
further
exploration
DPL5,
setting
stage
innovative,
nature-inspired
solutions
stubborn
bacterial
infections.
Microorganisms,
Год журнала:
2025,
Номер
13(5), С. 970 - 970
Опубликована: Апрель 24, 2025
Probiotic
species
have
garnered
significant
attention
for
their
health
benefits
extending
beyond
gastrointestinal
health.
This
study
investigated
the
biological
and
enzymatic
functions
of
selected
probiotic
species,
specifically
Lacticaseibacillus
rhamnosus
(formerly
Lactobacillus
rhamnosus),
Lactiplantibacillus
plantarum
plantarum),
acidophilus,
delbrueckii,
among
others,
through
in
vitro
experiments.
Enzymatic
activities,
including
hemolytic,
lipase,
esterase,
protease
functions,
were
evaluated.
Antioxidant
capacity
was
assessed
using
DPPH
radical
scavenging
assays,
while
antimicrobial
efficacy
tested
against
common
pathogenic
bacteria.
Antibiotic-resistance
patterns
analyzed
to
ascertain
safety
human
consumption.
Furthermore,
simulated
digestive
fluid
tolerance
experiments
conducted
evaluate
survival
tract.
The
findings
indicate
that
these
strains
exhibit
diverse
functionalities
intestinal
health,
with
potential
roles
digestion,
oxidative
stress
reduction,
immune
support.
provides
valuable
insights
into
functional
diversity
probiotics,
suggesting
broader
applications
nutrition.
Future
research
should
focus
on
vivo
validation,
mechanism
elucidation,
clinical
studies
determine
optimal
dosages
strain-specific
benefits.
