Network Pharmacology Insights into Broccoli Microgreens for Prostate Cancer

Heca Journal of Applied Sciences, Год журнала: 2025, Номер 3(1), С. 1 - 16

Опубликована: Фев. 26, 2025

Prostate cancer is a leading malignancy in men, ranking fourth globally and fifth Indonesia (GLOBOCAN 2020). Conventional therapies, though available, are limited by high costs, side effects, resistance, highlighting the need for accessible alternatives. Broccoli microgreens, rich bioactive compounds, have shown potential preventing treating various cancers. This study hypothesized that compounds broccoli microgreens interact with molecular targets involved prostate progression. To test this hypothesis, we employed network pharmacology-based silico approach to systematically explore these interactions identify therapeutic mechanisms. Bioactive were identified using liquid chromatography-mass spectrometry (LC-MS) analyzed via PubChem database. The biological activities of predicted PASS Online, focusing on their capacity modulate TP53 gene expression. Pharmacokinetic toxicity evaluations performed ADMETLab 3.0 Protox assess safety drug-like properties. Target proteins through SwissTargetPrediction GeneCards, while protein-protein interaction networks constructed STRING. pharmacological was visualized Cytoscape elucidate mechanisms action. analysis 528 relevant target proteins, key roles attributed SRC EGFR, both critical resistance EGFR tyrosine kinase inhibitors regulating processes such as cell proliferation, apoptosis metastatic potential. Through pharmacology, kaempferol polydatin targets, demonstrating ability pathways essential In conclusion, contain relevance cancer, particularly pathways, warranting further experimental validation.

Язык: Английский

Appraisal of Antioxidant Potential in Broccoli Microgreens under Different Drying Techniques Utilizing In Vitro and in Silico Methods

Malacca Pharmaceutics, Год журнала: 2025, Номер 3(1), С. 20 - 31

Опубликована: Янв. 17, 2025

Broccoli microgreens, rich in bioactive compounds, offer health benefits aligned with SDG 3: “Good Health and Well-Being.” Their antioxidants combat oxidative stress tied to chronic diseases, but drying can affect their activity. This study assessed the antioxidant capacities of fresh, microwave-dried, air-fryer-dried broccoli microgreens using vitro (DPPH assay) silico (molecular docking dynamics) methods. The were cultivated under controlled conditions dried microwave air-fryer techniques. Antioxidant activity was evaluated DPPH assay ethanolic extracts. compounds fresh detected through GC-MS, analyzed evaluate interactions target proteins CYP2C9 NOX2. findings revealed that demonstrated highest activity, followed by while microwave-dried samples exhibited lowest GC-MS analysis presence various including acids, ketones, sulfides, heterocycles, alcohols, esters, aromatic phthalate ester, aldehydes. Molecular strong certain NOX2, suggesting therapeutic potential against stress. dynamics simulations (MDS) showed stable binding for CYP2C9-Methyl myristate complex, NOX-(Z)-1,2-Diphenylethene complex displayed weaker stability. In conclusion, show mitigating stress, slightly enhancing capacity is comparable microgreens. analyses demonstrate between key implicated

Язык: Английский

The therapeutic potential of red radish microgreens in modulating inflammation and cancer pathways

CyTA - Journal of Food, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 20, 2025

Язык: Английский