Data-independent acquisition-based blood proteomics unveils predictive biomarkers for neonatal necrotizing enterocolitis DOI Creative Commons
Feng Chen,

Kezhe Tan,

Zhibao Lv

и другие.

Analytical and Bioanalytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 19, 2024

Abstract Necrotizing enterocolitis (NEC) is a life-threatening condition affecting preterm infants, sometimes necessitating surgical treatment. This study aimed to analyze differentially expressed proteins (DEPs) and access their biological clinical significance in the plasma of neonates with NEC. Peripheral blood samples were collected from NEC infants at various time points, was separated. Data-independent acquisition (DIA) technology utilized identify DEPs among patients different stages. Bioinformatic analyses, including Gene Ontology Kyoto Encyclopedia Genes Genomes, protein-to-protein interaction analyses performed on DEPs. External datasets, along receiver operating characteristic curves gene set enrichment analysis, used clinically biologically validate findings. between pre-NEC groups indicated reduced protein, heme, nitrogen, purine nucleotide biosynthesis during formation. In addition, enriched points suggested reconstructed extracellular matrix, aberrant B-lymphocyte immune responses, decreased glycosaminoglycan levels progression. These findings both validated using external datasets. Our highlights relevance proteomics patients. demonstrates key pathways involved pathogenesis establishes DIA mass spectrometry as powerful noninvasive tool for evaluating predicting formation Graphical

Язык: Английский

Iron-Mediated Regulation in Adipose Tissue: A Comprehensive Review of Metabolism and Physiological Effects DOI
Xinyu Yang, Xianghong Wang, Zhe Yang

и другие.

Current Obesity Reports, Год журнала: 2025, Номер 14(1)

Опубликована: Янв. 3, 2025

Язык: Английский

Процитировано

1

Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway DOI Creative Commons
Shubin Wang, Xiangjun Liu,

Lu Xu

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Янв. 30, 2025

Introduction Ulcerative colitis (UC) is a global gastrointestinal disease, which mainly caused by both dysfunctional epithelial barrier and inflammation response. Iron critical fundamental element for the maintenance of homeostasis mediation in many tissues. However, role mechanism iron phase enteritis subsequent repairing intestinal stem cells has not been elucidated. In this study, we aimed to explore whether how depletion would affect occurrence outcome experimental colitis. Methods was realized deferoxamine (DFO) at either early stage or late dextran sulfate sodium (DSS) induced mice. The gross images colons, general health, histology, integrity, qRT-PCR were performed. Meanwhile, cell culture colonic organoids used examine influence vitro . Signaling pathway inflammatory infiltration investigated immunostaining. Results within DSS treatment significantly inhibited onset response, maintained integrity epithelium, preserved activity (ISCs) vivo continuous DFO aggravated injury postponed recovery from Early DFO-induced able maintain p-STAT3 p-ERK1/2 signaling pathways epithelium colitis, but these two pathways. Discussion Our study demonstrated that manipulation might greatly outcomes phase-dependent manner, suggests balance metabolism be an effective therapeutic target clinical IBD patients.

Язык: Английский

Процитировано

0

Rocaglamide regulates iron homeostasis by suppressing hepcidin expression DOI
Xinyue Zhu,

Qi-Le Zuo,

Xueting Xie

и другие.

Free Radical Biology and Medicine, Год журнала: 2024, Номер 219, С. 153 - 162

Опубликована: Апрель 23, 2024

Язык: Английский

Процитировано

3

High-throughput point-of-care serum iron testing utilizing machine learning-assisted deep eutectic solvent fluorescence detection platform DOI
Hui Li, Han Yue, Haixiang Li

и другие.

Journal of Colloid and Interface Science, Год журнала: 2024, Номер 680, С. 389 - 404

Опубликована: Ноя. 17, 2024

Язык: Английский

Процитировано

2

Identification of iron metabolism-related genes in coronary heart disease and construction of a diagnostic model DOI Creative Commons
Lin Zhu,

Jianxin Zhang,

Wenhui Fan

и другие.

Frontiers in Cardiovascular Medicine, Год журнала: 2024, Номер 11

Опубликована: Ноя. 14, 2024

Background Coronary heart disease is a common cardiovascular disease, yferroptosiset its relationship with iron metabolism remains unclear. Methods Gene expression data from peripheral blood samples of patients coronary and healthy control group were utilized for comprehensive analysis that included differential analysis, weighted gene co-expression network enrichment the development logistic regression model to investigate associations differences between groups. Additionally, CIBERSORT algorithm was employed examine composition immune cell types within samples. Results Eight central genes identified as being both differentially expressed related metabolism. These are mainly involved in cellular stress response. A based on achieved an AUC 0.64–0.65 diagnosis disease. higher proportion M0 macrophages found while CD8T cells observed normal group. Conclusion The study important pathogenesis constructed robust diagnostic model. results suggest may play significant role providing basis further research.

Язык: Английский

Процитировано

1

Association between inflammatory cytokines and disease severity in restless legs syndrome DOI

Chaofan Geng,

Chen Chen

Neurological Sciences, Год журнала: 2024, Номер 45(12), С. 5765 - 5770

Опубликована: Авг. 29, 2024

Язык: Английский

Процитировано

0

DHA and EPA alleviate depressive-like behaviors in chronic sleep-deprived mice: Involvement of iron metabolism, oligodendrocyte-lipids peroxidation and the LCN2-NLRP3 signaling axis DOI
Lu Chen,

Yueqi Yang,

Ning Zhang

и другие.

Free Radical Biology and Medicine, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

0

Data-independent acquisition-based blood proteomics unveils predictive biomarkers for neonatal necrotizing enterocolitis DOI Creative Commons
Feng Chen,

Kezhe Tan,

Zhibao Lv

и другие.

Analytical and Bioanalytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 19, 2024

Abstract Necrotizing enterocolitis (NEC) is a life-threatening condition affecting preterm infants, sometimes necessitating surgical treatment. This study aimed to analyze differentially expressed proteins (DEPs) and access their biological clinical significance in the plasma of neonates with NEC. Peripheral blood samples were collected from NEC infants at various time points, was separated. Data-independent acquisition (DIA) technology utilized identify DEPs among patients different stages. Bioinformatic analyses, including Gene Ontology Kyoto Encyclopedia Genes Genomes, protein-to-protein interaction analyses performed on DEPs. External datasets, along receiver operating characteristic curves gene set enrichment analysis, used clinically biologically validate findings. between pre-NEC groups indicated reduced protein, heme, nitrogen, purine nucleotide biosynthesis during formation. In addition, enriched points suggested reconstructed extracellular matrix, aberrant B-lymphocyte immune responses, decreased glycosaminoglycan levels progression. These findings both validated using external datasets. Our highlights relevance proteomics patients. demonstrates key pathways involved pathogenesis establishes DIA mass spectrometry as powerful noninvasive tool for evaluating predicting formation Graphical

Язык: Английский

Процитировано

0